Searched for: faculty:"Applied%5C%2BSciences"
(1 - 17 of 17)
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Vos, T. (author), De la Torre Cortes, P. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Introduction: Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a net input of energy. To maximize the ATP yield on sugar in S. cerevisiae, industrial cultivation is typically performed in aerobic, sugar-limited fed-batch reactors which, due to constraints...
journal article 2015
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Kozak, B.U. (author)
Metabolic engineering – the improvement and addition, by genetic modification, of industrially relevant properties of microorganisms with respect to catalysis, transport and regulatory functions – is a well-established method for development of more cost-effective and ‘green’ industrial processes. Rapid depletion of oil reserves and a growing...
doctoral thesis 2015
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Oud, B. (author)
Reverse engineering is the study of discovering the structure, function and operation of a device or system with the express aim to reconstruct its key functionalities. This principle is applied to many disciplines, from military, through computer engineering, to health, but also in metabolic engineering. In this context, reverse metabolic...
doctoral thesis 2013
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Guadalupe Medina, V.G. (author)
In its search to decrease the environmental impact of the production of materials and food, and for other socio-economic reasons, mankind has recently taken the first steps into a paradigm shift from a petrochemical-based society to a new, sustainable and to a significant extent bio-based society. In this new scenario, biomass derived from...
doctoral thesis 2013
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Huisjes, E.H. (author)
The ambition to reduce our current dependence on fossil transportation fuels has driven renewed interest in bioethanol. Pectin-rich feedstocks like sugar beet pulp and citrus peel, which are currently sold as cattle feed, are promising raw materials for the production of bioethanol. This thesis explores the challenges related to the fermentation...
doctoral thesis 2013
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Guadalupe-Medina, V. (author), Wisselink, H.W. (author), Luttik, M.A.H. (author), De Hulster, E. (author), Daran, J.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Background Redox-cofactor balancing constrains product yields in anaerobic fermentation processes. This challenge is exemplified by the formation of glycerol as major by-product in yeast-based bioethanol production, which is a direct consequence of the need to reoxidize excess NADH and causes a loss of conversion efficiency. Enabling the use of...
journal article 2013
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Koopman, F.W. (author), Beekwilder, J. (author), Crimi, B. (author), Van Houwelingen, A. (author), Hall, R.D. (author), Bosch, D. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background Flavonoids comprise a large family of secondary plant metabolic intermediates that exhibit a wide variety of antioxidant and human health-related properties. Plant production of flavonoids is limited by the low productivity and the complexity of the recovered flavonoids. Thus to overcome these limitations, metabolic engineering of...
journal article 2012
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De Kok, S. (author)
Metabolic engineering – the improvement of cellular activities by manipulation of enzymatic, transport and regulatory functions of the cell – has enabled the industrial production of a wide variety of biological molecules from renewable resources. Microbial production of fuels and chemicals thereby provides an alternative to oil-based production...
doctoral thesis 2012
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Veiga, T. (author), Gombert, A.K. (author), Landes, N. (author), Verhoeven, M.D. (author), Kiel, J.A.K.W. (author), Krikken, A. (author), Nijland, J.G. (author), Touw, H. (author), Luttik, M.A.H. (author), Van der Toorn, J.C. (author), Driessen, A.J.M. (author), Bovenberg, R.A.L. (author), Van den Berg, M.A. (author), Van der Klei, I.J. (author), Pronk, J.T. (author), Daran, J.M. (author)
Industrial production of semi-synthetic cephalosporins by Penicillium chrysogenum requires supplementation of the growth media with the side-chain precursor adipic acid. In glucose-limited chemostat cultures of P. chrysogenum, up to 88% of the consumed adipic acid was not recovered in cephalosporin-related products, but used as an additional...
journal article 2012
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Daran, J.M. (author), Pronk, J.T. (author), Driessen, A.J.M. (author), Nijland, J.G. (author), Lamboo, F. (author), Puig-Martinez, M. (author), Veiga, T. (author), Gombert, A.K. (author)
Penicillium chrysogenum is widely used as an industrial antibiotic producer, in particular in the synthesis of ß-lactam antibiotics such as penicillins and cephalosporins. In industrial processes, oxalic acid formation leads to reduced product yields. Moreover, precipitation of calcium oxalate complicates product recovery. We observed oxalate...
journal article 2011
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Zelle, R.M. (author)
Biotechnological production of chemicals from renewable feedstocks offers a sustainable alternative to petrochemistry. Understanding of the biology of microorganisms and plants is increasing at an unprecedented rate and tools with which these organisms can be engineered for industrial application are becoming ever more powerful. However,...
doctoral thesis 2011
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Zhao, Z. (author), Kuijvenhoven, K. (author), Van Gulik, W.M. (author), Heijnen, J.J. (author), Van Winden, W.A. (author), Verheijen, P.J.T. (author)
The in vivo flux through the oxidative branch of the pentose phosphate pathway (oxPPP) in Penicillium chrysogenum was determined during growth in glucose/ethanol carbon-limited chemostat cultures, at the same growth rate. Non-stationary 13C flux analysis was used to measure the oxPPP flux. A nearly constant oxPPP flux was found for all glucose...
journal article 2010
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Verhoef, A. (author), Ballerstedt, H. (author), Volkers, R.J.M. (author), De Winde, J.H. (author), Ruijssenaars, H.J. (author)
A transcriptomics and proteomics approach was employed to study the expression changes associated with p-hydroxybenzoate production by the engineered Pseudomonas putida strain S12pa1B1. To establish p-hydroxybenzoate production, phenylalanine-tyrosine ammonia lyase (pal/tal) was introduced to connect the tyrosine biosynthetic and p-coumarate...
journal article 2010
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Harris, D.M. (author)
With an over 1000-fold improvement in specific productivity since its discovery, penicillin is one of the very successful examples of industrial biotechnology. Although classical strain improvement programmes have been a major contributor to this success, the wish for a more rational approach towards improvements has driven the work described in...
doctoral thesis 2008
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Kleijn, R.J. (author)
The 13C-labeling technique is a powerful characterization tool within the field of metabolic engineering aimed at determining intracellular steady state fluxes. The provided metabolic snapshots enable researchers to better understand and predict the phenotypic behavior of a micro-organism as a result of genetic alterations and/or different...
doctoral thesis 2007
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Geertman, J.M.A. (author)
Abstract not available
doctoral thesis 2006
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Kuijper, S.M. (author)
For various reasons mankind is looking for alternatives for fossil fuels. One of these alternatives is ethanol made from plant biomass. However, the plant material when broken down by hydrolysis into its sugar monomers contains a significant amount of xylose, a 5-carbon-sugar or pentose. Contrary to the sugars with 6 carbon atoms (hexoses) wild...
doctoral thesis 2006
Searched for: faculty:"Applied%5C%2BSciences"
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