Searched for: faculty:"Applied%5C%2BSciences"
(1 - 20 of 42)

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Righolt, C.H. (author), Guffei, A. (author), Knecht, H. (author), Young, I.T. (author), Stallinga, S. (author), Van Vliet, L.J. (author), Mai, S. (author)
Advances in light microscopy have enabled the visualization of DNA in the interphase nucleus with more detail than is visible with conventional light microscopy. The nuclear architecture is assumed to be different in cancer cells compared to normal cells. In this paper we have studied, for the first time, the organization of nuclear DNA and that...
journal article 2014
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Righolt, C.H. (author), Slotman, J.A. (author), Young, I.T. (author), Mai, S. (author), Van Vliet, L.J. (author), Stallinga, S. (author)
Various aspects of image filtering affect the final image quality in Structured Illumination Microscopy, in particular the regularization parameter and type of regularization function, the relative height of the side bands, and the shape of the apodization function. We propose an apodization filter without adjustable parameters based on the...
journal article 2013
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Lindner, M. (author), Nir, G. (author), Vivante, A. (author), Young, I.T. (author), Garini, Y. (author)
The dynamics of a diffusing particle in a potential field is ubiquitous in physics, and it plays a pivotal role in single-molecule studies. We present a formalism for analyzing the dynamics of diffusing particles in harmonic potentials at low Reynolds numbers using the time evolution of the particle probability distribution function. We...
journal article 2013
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Zhao, Q. (author), Young, I.T. (author), De Jong, J.G.S. (author)
We have constructed a mathematical model to analyze the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy (FLIM). The power of the light source needed for illumination in a FLIM system and the signal-to-noise ratio of the detector have led us to a photon “budget.” These measures are relevant to many fluorescence...
journal article 2011
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Righolt, C.H. (author), Raz, V. (author), Vermolen, B.J. (author), Dirks, R.W. (author), Tanke, H.J. (author), Young, I.T. (author)
The nuclear lamina is an intermediate filament network that provides a structural framework for the cell nucleus. Changes in lamina structure are found during changes in cell fate such as cell division or cell death and are associated with human diseases. An unbiased method that quantifies changes in lamina shape can provide information on cells...
journal article 2011
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Dietrich, H.R.C. (author), Rieger, B. (author), Wiertz, F.G.M. (author), De Groote, F.H. (author), Heering, H.A. (author), Young, I.T. (author), Garini, Y. (author)
journal article 2009
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Brinkers, S. (author), Dietrich, H.R.C. (author), De Groote, F.H. (author), Young, I.T. (author), Rieger, B. (author)
The wormlike chain model describes the micromechanics of semiflexible polymers by introducing the persistence length. We propose a method of measuring the persistence length of DNA in a controllable near-native environment. Using a dark field microscope, the projected positions of a gold nanoparticle undergoing constrained Brownian motion are...
journal article 2009
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Young, T. (author)
public lecture 2009
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Young, I.T. (author), El Morabit, M. (author), Liqui Lung, G. (author), Vermolen, B.J. (author)
Based upon a collection of compact LEDs (light-emitting diodes) and a compact photodiode, we have developed a calibration tool for fluorescence microscopes that are used as digital imaging devices. The entire device (excluding a USB connector) measures 25 mm x 80 mm x 12 mm. Virtually all commonly-used fluorophores can be simulated with one of...
conference paper 2008
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Docter, M.W. (author), Van den Berg, P.M. (author), Alkemade, P.F.A. (author), Kutchoukov, V.G. (author), Piciu, O.M. (author), Bossche, A. (author), Young, I.T. (author), Garini, Y. (author)
journal article 2007
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Dietrich, H.R.C. (author), Vermolen, B.J. (author), Rieger, B. (author), Young, I.T. (author), Garini, Y. (author)
Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been...
conference paper 2007
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Docter, M.W. (author), Young, I.T. (author), Piciu, O.M. (author), Bossche, A. (author), Alkemade, P.F.A. (author), Van den Berg, P.M. (author), Garini, Y. (author)
We present a study on the far-field patterns of light transmitted through sub-wavelength metallic hole-arrays. Spectral imaging measurements are used here on hole arrays for the first time. It provides both spatial and spectral information of the transmission in far-field. The visibility of the images, measured in two illumination modes: Köhler...
journal article 2006
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Docter, M.W. (author), Young, I.T. (author), Piciu, O. (author), Bossche, A. (author), Alkemade, P.F.A. (author), Van de Berg, P.M. (author), Garini, Y. (author)
The knowledge of the near-field of extra-ordinary transmission through hole-arrays is mostly theoretical; there is less experimental validation of the theory. We study the near-field properties by measuring fluorescent molecules that are immersed in a solution and their Brownian motion. The measurements are performed by filling the space above...
conference paper 2006
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Dietrich, H.R.C. (author), Young, I.T. (author), Garini, Y. (author)
The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact...
conference paper 2006
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Young, I.T. (author), Garini, Y. (author), Vermolen, B. (author), Liqui Lund, G. (author), Brouwer, G. (author), Hendrichs, S. (author), El Morabit, M. (author), Spoelstra, J. (author), Wilhelm, E. (author), Zaal, M. (author)
While fluorescence microscope systems remains an essential tool in modern biology and medical work, no compact instrumentation has been developed for the rapid calibration of such systems. Almost invariably results are presented in terms of the [AU], "arbitrary units". To remedy this situation we have developed a small, portable instrument - the...
conference paper 2006
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Garini, Y. (author), Young, I.T. (author)
The invention relates to an optical microscope, comprising, at least a light source, a carrier for an object to be examined, a detector for registering the illuminated object, and a light path that during operation runs substantially from the light source to the object and form the object to the detector, wherein a metallic film having a...
patent 2005
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Dietrich, H.R.C. (author), Van den Doel, R.L. (author), Hoyer, W. (author), Van Oel, W. (author), Liqui Lung, G. (author), Garini, Y. (author), Jovin, T. (author), Young, I.T. (author)
In previous publications we have shown that we can perform enzymatic reactions in nanoarrays by means of a microarray-reader based on a conventional microscope. In this publication we report on a modification of this system in order to monitor the aggregation kinetics of the natively unfolded protein ?-synuclein. We describe the motivation for...
conference paper 2005
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Vermolen, B.J. (author), Young, I.T. (author), Chuang, A. (author), Wark, L. (author), Chuang, T. (author), Mai, S. (author), Garini, Y. (author)
Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we...
conference paper 2005
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Dietrich, H.R.C. (author), Young, I.T. (author), Garini, Y. (author)
The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a...
conference paper 2005
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Docter, M.W. (author), Young, I.T. (author), Kutchoukov, V.G. (author), Bossche, A. (author), Alkemade, P.F.A. (author), Garini, Y. (author)
When light passes through a hole smaller than the wavelength of the light, the transmission is very low and the light is diffracted. This however changes if holes are arranged in a periodic array on metal. In that case the light couples to surface plasmons; this results in enhanced transmission, spectral selection and a small angular diffraction...
conference paper 2005
Searched for: faculty:"Applied%5C%2BSciences"
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