Searched for: faculty:"Applied%5C%2BSciences"
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Laan, A.C. (author), Santini, C. (author), Jennings, L. (author), De Jong, M. (author), Bernsen, M.R. (author), Denkova, A.G. (author)
Background Single photon emission computed tomography (SPECT) is an indispensable tool in the determination of the in vivo fate of polymeric micelles. However, for this purpose, the micelles need to be radiolabeled, and almost all radiolabeling procedures published to date involve the conjugation of a chelating agent to the constituting polymer,...
journal article 2016
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Prest, E.I.E.D. (author), Hammes, F. (author), Van Loosdrecht, M.C.M. (author), Vrouwenvelder, J.S. (author)
Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of...
journal article 2016
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Moerland, R.J. (author), Hoogenboom, J.P. (author)
Available data: Complex refractive index of Indium Tin Oxide, http://dx.doi.org/10.4121/uuid:59febf27-a532-4ac9-8ec0-29d4195b2c8c Transparent conductive oxides (TCOs), such as the well-known indium-tin oxide, find widespread use in modern (nano)technological applications because of their unique combination of negligible optical absorption and...
journal article 2016
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Schumacher, R. (author), Wahl, S.A. (author)
The design of microbial production processes relies on rational choices for metabolic engineering of the production host and the process conditions. These require a systematic and quantitative understanding of cellular regulation. Therefore, a novel method for dynamic flux identification using quantitative metabolomics and 13C labeling to...
journal article 2015
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Milne, N. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background: Decarboxylation of ?-ketoisovalerate to isobutyraldehyde is a key reaction in metabolic engineering of Saccharomyces cerevisiae for isobutanol production with published studies relying on overexpression of either the native ARO10 gene or of the Lactococcus lactis kivD decarboxylase gene resulting in low enzymatic activities. Here, we...
journal article 2015
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Vlijm, R. (author), Torre, J. (author), Dekker, C. (author)
The metabolism of DNA in cells relies on the balance between hybridized double-stranded DNA (dsDNA) and local de-hybridized regions of ssDNA that provide access to binding proteins. Traditional melting experiments, in which short pieces of dsDNA are heated up until the point of melting into ssDNA, have determined that AT-rich sequences have a...
journal article 2015
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Vlijm, R. (author), Lee, M. (author), Ordu, O. (author), Boltengagen, A. (author), Lusser, A. (author), Dekker, N.H. (author), Dekker, C. (author)
Eukaryotic nucleosomes consists of an (H3-H4)2 tetramer and two H2A-H2B dimers, around which 147 bp of DNA are wrapped in 1.7 left-handed helical turns. During chromatin assembly, the (H3-H4)2 tetramer binds first, forming a tetrasome that likely constitutes an important intermediate during ongoing transcription. We recently showed that (H3-H4)2...
journal article 2015
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Nemati, M. (author), Loozen, G.B. (author), Van der Wekken, N. (author), Van de Belt, G. (author), Urbach, H.P. (author), Bhattacharya, N. (author), Kenjeres, S. (author)
A preliminary comparative measurement between particle imaging velocimetry (PIV) and laser speckle contrast analysis (LASCA) to study pulsatile flow using ventricular assist device in a patient-specific carotid artery phantom is reported. These full-field optical techniques have both been used to study flow and extract complementary parameters....
journal article 2015
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Sorokin, D.Y. (author), Toshchakov, S.V. (author), Kolganova, T.V. (author), Kublanov, I.V. (author)
Until recently, extremely halophilic euryarchaeota were considered mostly as aerobic heterotrophs utilizing simple organic compounds as growth substrates. Almost nothing is known on the ability of these prokaryotes to utilize complex polysaccharides, such as cellulose, xylan, and chitin. Although few haloarchaeal cellulases and chitinases were...
journal article 2015
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Frisenda, R. (author), Parlato, L. (author), Barra, M. (author), Van der Zant, H.S.J. (author), Cassinese, A. (author)
In this letter, we report the single-molecule conductance properties of a cyano-functionalized perylene-diimide derivative (PDI8-CN 2 ) investigated with gold nano-electrodes. This molecule is of large interest for the fabrication of high-performance and air-stable n-type organic field-effect transistors. Low-bias experiments performed on...
journal article 2015
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Heeres, A.S. (author), Schroën, K. (author), Heijnen, J.J. (author), Van der Wielen, L.A.M. (author), Cuellar, M.C. (author)
Developments in synthetic biology enabled the microbial production of long chain hydrocarbons, which can be used as advanced biofuels in aviation or transportation. Currently, these fuels are not economically competitive due to their production costs. The current process offers room for improvement: by utilizing lignocellulosic feedstock,...
journal article 2015
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Lin, Y.M. (author), Nierop, K.G.J. (author), Girbal-Neuhauser, E. (author), Adriaanse, M. (author), Van Loosdrecht, M.C.M. (author)
To evaluate the possibility of utilizing polysaccharide-based biomaterial recovered from aerobic granular sludge as a coating material, the morphology, molecular weight distribution and chemical composition of the recovered biomaterial were investigated by atomic force microscopy, size exclusion chromatography and pyrolysis–GC–MS to have a...
journal article 2015
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Nieuwenhuizen, R.P.J. (author), Nahidiazar, L. (author), Manders, E.M.M. (author), Jalink, K. (author), Stallinga, S. (author), Rieger, B. (author)
Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of...
journal article 2015
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Krishnan, S.T. (author), Moolman, M.C. (author), Van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)
Background Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date, ?...
journal article 2015
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Hol, F.J.H. (author), Galajda, P. (author), Woolthuis, R.G. (author), Dekker, C. (author), Keymer, J.E. (author)
Background The spatial structure of a habitat can have a strong impact on community dynamics. Different experimental approaches exist to explore the effect of spatial structure on bacterial communities. To investigate the effect of ‘space’, a single implementation of spatial structure is often contrasted to bacterial community dynamics in well...
journal article 2015
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De Kruijff, R.M. (author), Wolterbeek, H.T. (author), Denkova, A.G. (author)
This review presents an overview of the successes and challenges currently faced in alpha radionuclide therapy. Alpha particles have an advantage in killing tumour cells as compared to beta or gamma radiation due to their short penetration depth and high linear energy transfer (LET). Touching briefly on the clinical successes of radionuclides...
journal article 2015
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Pronk, M. (author), Abbas, B. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)
The influence of sludge age on granular sludge formation and microbial population dynamics in a methanol- and acetate-fed aerobic granular sludge system operated at 35°C was investigated. During anaerobic feeding of the reactor, methanol was initially converted to methane by methylotrophic methanogens. These methanogens were able to withstand...
journal article 2015
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average number of localizations per fluorophore, or...
journal article 2015
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Pérez Garza, H. (author), Ghatkesar, M.K. (author), Basak, S. (author), Löthman, P. (author), Staufer, U. (author)
To manipulate liquid matter at the nanometer scale, we have developed a robotic assembly equipped with a hollow atomic force microscope (AFM) cantilever that can handle femtolitre volumes of liquid. The assembly consists of four independent robots, each sugar cube sized with four degrees of freedom. All robots are placed on a single platform...
journal article 2015
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Janulevicius, A. (author), Van Loosdrecht, M.C.M. (author), Picioreanu, C. (author)
Myxobacteria are social bacteria that upon starvation form multicellular fruiting bodies whose shape in different species can range from simple mounds to elaborate tree-like structures. The formation of fruiting bodies is a result of collective cell movement on a solid surface. In the course of development, groups of flexible rod-shaped cells...
journal article 2015
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