document

A secretomic view of woody and nonwoody lignocellulose degradation by Pleurotus ostreatus

Fernandez Fueyo, E. (author), Ruiz-Duenas, F.J. (author), Lopez-Lucendo, M.F. (author), Perez-Boada, M. (author), Rencorat, J. (author), Gutierrez, A. (author), Pisabarro, A.G. (author), Ramirez, L. (author), Martinez, A.T. (author)

Background: Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose...

journal article 2016

document

Improving health in cities through systems approaches for urban water management

Rietveld, L.C. (author), Siri, J.G. (author), Chakravarty, I. (author), Arsenio, A.M. (author), Biswas, R. (author), Chatterjee, A. (author)

Background: As human populations become more and more urban, decision-makers at all levels face new challenges related to both the scale of service provision and the increasing complexity of cities and the networks that connect them. These challenges may take on unique aspects in cities with different cultures, political and institutional...

conference paper 2016

document

Growth-rate dependency of de novo resveratrol production in chemostat cultures of an engineered Saccharomyces cerevisiae strain

Vos, T. (author), De la Torre Cortes, P. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)

Introduction: Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a net input of energy. To maximize the ATP yield on sugar in S. cerevisiae, industrial cultivation is typically performed in aerobic, sugar-limited fed-batch reactors which, due to constraints...

journal article 2015

document

Comparative assessment of native and heterologous 2-oxo acid decarboxylases for application in isobutanol production by Saccharomyces cerevisiae

Milne, N. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)

Background: Decarboxylation of ?-ketoisovalerate to isobutyraldehyde is a key reaction in metabolic engineering of Saccharomyces cerevisiae for isobutanol production with published studies relying on overexpression of either the native ARO10 gene or of the Lactococcus lactis kivD decarboxylase gene resulting in low enzymatic activities. Here, we...

journal article 2015

document

Microbial population dynamics during long-term sludge adaptation of thermophilic and mesophilic sequencing batch digesters treating sewage fine sieved fraction at varying organic loading rates

Tao, Y. (author), De Kreuk, M.K. (author), Zandvoort, M.H. (author), Van Lier, J.B. (author)

Background In this research, the feasibility of, and population dynamics in, one-step anaerobic sequencing batch reactor systems treating the fine sieved fraction (FSF) from raw municipal wastewater was studied under thermophilic (55 °C) and mesophilic (35 °C) conditions. FSF was sequestered from raw municipal wastewater, in the Netherlands,...

journal article 2015

document

Design and pilot validation of A-gear: A novel wearable dynamic arm support (+ Erratum)

Kooren, P.N. (author), Dunning, A.G. (author), Janssen, M.M.H.P. (author), Lobo-Prat, J. (author), Koopman, B.F.J.M. (author), Paalman, M.I. (author), de Groot, I.J.M. (author), Herder, J.L. (author)

Background Persons suffering from progressive muscular weakness, like those with Duchenne muscular dystrophy (DMD), gradually lose the ability to stand, walk and to use their arms. This hinders them from performing daily activities, social participation and being independent. Wheelchairs are used to overcome the loss of walking. However, there...

journal article 2015

document

Reflections on the role of energy network companies in the energy transition

Steenhuisen, B.M. (author), De Bruijne, M.L.C. (author)

Background Energy network companies are commonly attributed the public task to help society in becoming greener in the domain of energy. This extra public task has high costs and comes with high uncertainties. It may also compete with existing public tasks of network companies. When network companies are involved in local green initiatives, they...

journal article 2015

document

Single-Molecule Break Junctions Based on a Perylene-Diimide Cyano-Functionalized (PDI8-CN2) Derivative

Frisenda, R. (author), Parlato, L. (author), Barra, M. (author), Van der Zant, H.S.J. (author), Cassinese, A. (author)

In this letter, we report the single-molecule conductance properties of a cyano-functionalized perylene-diimide derivative (PDI8-CN 2 ) investigated with gold nano-electrodes. This molecule is of large interest for the fabrication of high-performance and air-stable n-type organic field-effect transistors. Low-bias experiments performed on...

journal article 2015

document

Extending bioenergy towards smart biomass use Issues of social acceptance at Park Cuijk, The Netherlands

Ganzevles, J. (author), Asveld, L. (author), Osseweijer, P. (author)

Background While the share of bioenergy in the overall energy supply has increased over the last decade, its social acceptance is fragile, mainly due to concerns about negative sustainability impacts. In this paper, we will investigate to what extent the extension of bioenergy towards ‘smart’ or ‘cascaded’ biomass use enhances a project’s social...

journal article 2015

document

Essential validation methods for E. coli strains created by chromosome engineering

Krishnan, S.T. (author), Moolman, M.C. (author), Van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)

Background Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date, ?...

journal article 2015

document

The idiosyncrasy of spatial structure in bacterial competition

Hol, F.J.H. (author), Galajda, P. (author), Woolthuis, R.G. (author), Dekker, C. (author), Keymer, J.E. (author)

Background The spatial structure of a habitat can have a strong impact on community dynamics. Different experimental approaches exist to explore the effect of spatial structure on bacterial communities. To investigate the effect of ‘space’, a single implementation of spatial structure is often contrasted to bacterial community dynamics in well...

journal article 2015

document

XNAT imaging platform for BioMedBridges and CTMM TraIT (meeting abstract)

Klein, S. (author), Vast, E. (author), Van Soest, J. (author), Dekker, A. (author), Koek, M. (author), Niessen, W.J. (author)

1st Clinical Research Informatics (CRI) Solutions Day Duesseldorf, Germany, 26-27 May 2014

journal article 2015

document

Geometric optimization of an ultralow-dose high-resolution pediatric PET scanner based on monolithic scintillators with dSiPM readout (meeting abstract)

Mikhaylova, E. (author), Tabacchini, V. (author), Borghi, G. (author), Mollet, P. (author), D'Hoe, E. (author), Schaart, D.R. (author), Vandenberghe, S. (author)

PSMR 2015: 4th Conference on PET/MR and SPECT/MR, La Biodola, Isola d’Elba, Italy, 17-21 May 2015

journal article 2015

document

Fully automatic evaluation of the corneal endothelium from in vivo confocal microscopy

Selig, B. (author), Vermeer, K.A. (author), Rieger, B. (author), Hillenaar, T. (author), Hendriks, C.L.L. (author)

Background Manual and semi-automatic analyses of images, acquired in vivo by confocal microscopy, are often used to determine the quality of corneal endothelium in the human eye. These procedures are highly time consuming. Here, we present two fully automatic methods to analyze and quantify corneal endothelium imaged by in vivo white light slit...

journal article 2015

document

An integrated approach for efficient biomethane production from solid bio-wastes in a compact system

Wang, H. (author), Tao, Y. (author), Temudo, M. (author), Schooneveld, M. (author), Bijl, H. (author), Ren, N. (author), Wolf, M. (author), Heine, C. (author), Foerster, A. (author), Pelenc, V. (author), Kloek, J. (author), Van Lier, J.B. (author), De Kreuk, M.K. (author)

Background Solid bio-wastes (or organic residues) are worldwide produced in high amount and increasingly considered bioenergy containers rather than waste products. A complete bioprocess from recalcitrant solid wastes to methane (SW2M) via anaerobic digestion (AD) is believed to be a sustainable way to utilize solid bio-wastes. However, the...

journal article 2015

document

Test method to assess interface adhesion in composite bonding

Teixeira de Freitas, S. (author), Sinke, J. (author)

This paper introduces a new type of peel tests dedicated to composite bonding: Composite Peel Tests. This test is inspired on the standard floating roller peel test widely used for metal bonding. The aim of this study is to investigate the potential of the Composite Peel Test to assess interface adhesion in composite bonded structures. To this...

journal article 2015

document

The effects of observation of walking in a living room environment, on physical, cognitive, and quality of life related outcomes in older adults with dementia: A study protocol of a randomized controlled trial

Douma, J.G. (author), Volkers, K.M. (author), Vuijk, J.P. (author), Sonneveld, M.H. (author), Goossens, R.H.M. (author), Scherder, E.J.A. (author)

Background The number of older adults with dementia is expected to increase. Dementia is not only characterized by a decline in cognition, also other functions, for example, physical functioning change. A possible means to decrease the decline in these functions, or even improve them, could be increasing the amount of physical activity. A...

journal article 2015

document

Comprehensive neuromechanical assessment in stroke patients: Reliability and responsiveness of a protocol to measure neural and non-neural wrist properties

Van der Krogt, H. (author), Klomp, A. (author), De Groot, J.H. (author), De Vlugt, E. (author), Van der Helm, F.C.T. (author), Meskers, C.G.M. (author), Arendzen, J.H. (author)

Background: Understanding movement disorder after stroke and providing targeted treatment for post stroke patients requires valid and reliable identification of biomechanical (passive) and neural (active and reflexive) contributors. Aim of this study was to assess test-retest reliability of passive, active and reflexive parameters and to...

journal article 2015

document

Lipid recovery from a vegetable oil emulsion using microbial enrichment cultures

Tamis, J. (author), Sorokin, D.Y. (author), Jiang, Y. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)

Background Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil...

journal article 2015

document

Early motor learning changes in upper-limb dynamics and shoulder complex loading during handrim wheelchair propulsion

Vegter, R.J.K. (author), Hartog, J. (author), De Groot, S. (author), Lamoth, C.J. (author), Bekker, M.J. (author), Van der Scheer, J.W. (author), Van der Woude, L.H.V. (author), Veeger, H.E.J. (author)

Background To propel in an energy-efficient manner, handrim wheelchair users must learn to control the bimanually applied forces onto the rims, preserving both speed and direction of locomotion. Previous studies have found an increase in mechanical efficiency due to motor learning associated with changes in propulsion technique, but it is...

journal article 2015