Fabrication methods of a novel 3D hybrid stiffness scaffold for neuron mechanosensing

Two-photon polymerization-based 3D-multi-electrode arrays for electrical monitoring of neuronal cells

3D-multi-electrode arrays (3D-MEAs) are needed to overcome the limitations of 2D-multi-electrode arrays (2D-MEAs) and enable the electrical characterization of 3D neuronal cultures in in vitro brain models, advancing the understanding of neurological disorders and paving the way to personalized medicine. The aim of this thesis was to overcome...

Liquid dosing on the micro-scale: A quest for increased resolution

The processes that take place within cells are complex. Single-cell analysis is a method that is employed to gain further understanding of the working mechanisms on a single-cellular level. The controlled transport of substances through the cell membrane is important for studying the behaviour and responses of single living cells. Femtopipettes...

Multiscale 3D printed polymer probes for single cell experiments: A rapid prototyping method to fabricate microfluidic atomic force microscopy cantilevers for single cell studies

Facioscapulohumeral muscle dystrophy (FSHD) is the third most common muscle disease in the world. No cure has been found for FSHD, and current treatments focus on alleviating the symptoms. The disease is caused by a genetic error in 1/1000 - 1/200 of the nuclei in a multinucleated skeletal muscle cell. Studying that specific nucleus, by removing...