Searched for: subject%3A%22Engineering%22
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Jaroensuk, Juthamas (author), Sutthaphirom, Chalermroj (author), Phonbuppha, Jittima (author), Chinantuya, Wachirawit (author), Kesornpun, Chatchai (author), Akeratchatapan, Nattanon (author), Kittipanukul, Narongyot (author), Phatinuwat, Kamonwan (author), Hollmann, F. (author)
Cofactor imbalance obstructs the productivities of metabolically engineered cells. Herein, we employed a minimally perturbing system, xylose reductase and lactose (XR/lactose), to increase the levels of a pool of sugar phosphates which are connected to the biosynthesis of NAD(P)H, FAD, FMN, and ATP in Escherichia coli. The XR/lactose system...
journal article 2024
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van den Broek, M.A. (author), Ortiz Merino, R.A. (author), Bennis, N.X. (author), Wronska, A.K. (author), Else-Hassing, J. (author), Daran-Lapujade, P.A.S. (author), Daran, J.G. (author)
The biobased-economy aims to create a circular biotechnology ecosystem to transition from a fossil fuel-based to a sustainable industry based on biomass. For this, new microbial cell-factories are essential. We present the draft genome of the CEN.PK-derived Saccharomyces cerevisiae SpyCas9 expressing strain (IMX2600), that serve as chassis of...
journal article 2024
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van Lent, P.H. (author), Schmitz, Joep (author), Abeel, T.E.P.M.F. (author)
Combinatorial pathway optimization is an important tool in metabolic flux optimization. Simultaneous optimization of a large number of pathway genes often leads to combinatorial explosions. Strain optimization is therefore often performed using iterative design-build-test-learn (DBTL) cycles. The aim of these cycles is to develop a product...
journal article 2023
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Perli, T. (author)
The increase in world population together with an unsustainable and fossil fuel-based economy are at the root of the significant increase in the average global temperature since the pre-industrial era. Switching to a more sustainable and circular bio-based economy is one of the main pillars included in the Paris agreement aimed at reducing green...
doctoral thesis 2021
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Wronska, A.K. (author), Haak, Meinske P. (author), Geraats, Ellen (author), Bruins Slot, Eva (author), van den Broek, M.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Biotin, an important cofactor for carboxylases, is essential for all kingdoms of life. Since native biotin synthesis does not always suffice for fast growth and product formation, microbial cultivation in research and industry often requires supplementation of biotin. De novo biotin biosynthesis in yeasts is not fully understood, which...
journal article 2020
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Xiberras, Joeline (author), Klein, Mathias (author), de Hulster, A.F. (author), Mans, R. (author), Nevoigt, Elke (author)
Previously, our lab replaced the endogenous FAD-dependent pathway for glycerol catabolism in S. cerevisiae by the synthetic NAD-dependent dihydroxyacetone (DHA) pathway. The respective modifications allow the full exploitation of glycerol’s higher reducing power (compared to sugars) for the production of the platform chemical succinic acid ...
journal article 2020
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Shomar Monges, H. (author)
For millennia, humans have used microbes to produce industrial products of social and economical value through fermentation processes. In recent years, the application of engineering principles to microbiology have dramatically expanded our ability to modify and optimize microbes for the production of a wide variety of commercial products from...
doctoral thesis 2019
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Levisson, Mark (author), Araya-Cloutier, Carla (author), De Bruijn, Wouter J.C. (author), Van Der Heide, Menno (author), Salvador López, José Manuel (author), Daran, J.G. (author), Vincken, Jean Paul (author), Beekwilder, Jules (author)
Prenylated flavonoids possess a wide variety of biological activities, including estrogenic, antioxidant, antimicrobial, and anticancer activities. Hence, they have potential applications in food products, medicines, or supplements with health-promoting activities. However, the low abundance of prenylated flavonoids in nature is limiting...
journal article 2019
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Jansen, Mickel L.A. (author), Bracher, J.M. (author), Papapetridis, I. (author), Verhoeven, M.D. (author), de Bruijn, J.A. (author), de Waal, P. (author), van Maris, A.J.A. (author), Klaassen, P (author), Pronk, J.T. (author)
The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains,...
journal article 2017
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Vos, T. (author), De la Torre Cortes, P. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Introduction: Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a net input of energy. To maximize the ATP yield on sugar in S. cerevisiae, industrial cultivation is typically performed in aerobic, sugar-limited fed-batch reactors which, due to constraints...
journal article 2015
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Kozak, B.U. (author)
Metabolic engineering – the improvement and addition, by genetic modification, of industrially relevant properties of microorganisms with respect to catalysis, transport and regulatory functions – is a well-established method for development of more cost-effective and ‘green’ industrial processes. Rapid depletion of oil reserves and a growing...
doctoral thesis 2015
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Oud, B. (author)
Reverse engineering is the study of discovering the structure, function and operation of a device or system with the express aim to reconstruct its key functionalities. This principle is applied to many disciplines, from military, through computer engineering, to health, but also in metabolic engineering. In this context, reverse metabolic...
doctoral thesis 2013
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Guadalupe Medina, V.G. (author)
In its search to decrease the environmental impact of the production of materials and food, and for other socio-economic reasons, mankind has recently taken the first steps into a paradigm shift from a petrochemical-based society to a new, sustainable and to a significant extent bio-based society. In this new scenario, biomass derived from...
doctoral thesis 2013
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Huisjes, E.H. (author)
The ambition to reduce our current dependence on fossil transportation fuels has driven renewed interest in bioethanol. Pectin-rich feedstocks like sugar beet pulp and citrus peel, which are currently sold as cattle feed, are promising raw materials for the production of bioethanol. This thesis explores the challenges related to the fermentation...
doctoral thesis 2013
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Guadalupe-Medina, V. (author), Wisselink, H.W. (author), Luttik, M.A.H. (author), De Hulster, E. (author), Daran, J.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Background Redox-cofactor balancing constrains product yields in anaerobic fermentation processes. This challenge is exemplified by the formation of glycerol as major by-product in yeast-based bioethanol production, which is a direct consequence of the need to reoxidize excess NADH and causes a loss of conversion efficiency. Enabling the use of...
journal article 2013
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Koopman, F.W. (author), Beekwilder, J. (author), Crimi, B. (author), Van Houwelingen, A. (author), Hall, R.D. (author), Bosch, D. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background Flavonoids comprise a large family of secondary plant metabolic intermediates that exhibit a wide variety of antioxidant and human health-related properties. Plant production of flavonoids is limited by the low productivity and the complexity of the recovered flavonoids. Thus to overcome these limitations, metabolic engineering of...
journal article 2012
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De Kok, S. (author)
Metabolic engineering – the improvement of cellular activities by manipulation of enzymatic, transport and regulatory functions of the cell – has enabled the industrial production of a wide variety of biological molecules from renewable resources. Microbial production of fuels and chemicals thereby provides an alternative to oil-based production...
doctoral thesis 2012
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Veiga, T. (author), Gombert, A.K. (author), Landes, N. (author), Verhoeven, M.D. (author), Kiel, J.A.K.W. (author), Krikken, A. (author), Nijland, J.G. (author), Touw, H. (author), Luttik, M.A.H. (author), Van der Toorn, J.C. (author), Driessen, A.J.M. (author), Bovenberg, R.A.L. (author), Van den Berg, M.A. (author), Van der Klei, I.J. (author), Pronk, J.T. (author), Daran, J.M. (author)
Industrial production of semi-synthetic cephalosporins by Penicillium chrysogenum requires supplementation of the growth media with the side-chain precursor adipic acid. In glucose-limited chemostat cultures of P. chrysogenum, up to 88% of the consumed adipic acid was not recovered in cephalosporin-related products, but used as an additional...
journal article 2012
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Daran, J.M. (author), Pronk, J.T. (author), Driessen, A.J.M. (author), Nijland, J.G. (author), Lamboo, F. (author), Puig-Martinez, M. (author), Veiga, T. (author), Gombert, A.K. (author)
Penicillium chrysogenum is widely used as an industrial antibiotic producer, in particular in the synthesis of ß-lactam antibiotics such as penicillins and cephalosporins. In industrial processes, oxalic acid formation leads to reduced product yields. Moreover, precipitation of calcium oxalate complicates product recovery. We observed oxalate...
journal article 2011
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Zelle, R.M. (author)
Biotechnological production of chemicals from renewable feedstocks offers a sustainable alternative to petrochemistry. Understanding of the biology of microorganisms and plants is increasing at an unprecedented rate and tools with which these organisms can be engineered for industrial application are becoming ever more powerful. However,...
doctoral thesis 2011
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