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Verhoeven, M.D. (author), Bracher, J.M. (author), Nijland, Jeroen G. (author), Bouwknegt, J. (author), Daran, J.G. (author), Driessen, Arnold J.M. (author)Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. This strain, which showed a growth rate of 0.26 h−1 on l-arabinose in aerobic batch cultures, was subsequently...journal article 2018
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Oosting, R.M. (author), Dankelman, J. (author), Wauben, L.S.G.L. (author), Madete, J. (author), Groen, R. S. (author)Safe and affordable surgery is not accessible for five billion people when they need it. Multiple surgical capacity studies have shown that hospitals in low-And-middle income countries do not have complete coverage of basic surgical equipment such as, theatre lights, anesthesia machines and electro surgical units. Currently, almost all...conference paper 2018
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Xu, L. (author), Zhai, Wan Ming (author)To efficiently select track irregularity random samples for satisfying the ergodicity requirements of excitation sources in stochastic dynamics and reliability analysis in vehicle-track system, the weak-stationarity and similarity spectral of track irregularities were introduced to propose a track irregularity probabilistic model. Using the...journal article 2018
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Abbink, D.A. (author), Carlson, Tom (author), Mulder, M. (author), de Winter, J.C.F. (author), Aminravan, Farzad (author), Gibo, T.L. (author), Boer, E.R. (author)Shared control is an increasingly popular approach to facilitate control and communication between humans and intelligent machines. However, there is little consensus in guidelines for design and evaluation of shared control, or even in a definition of what constitutes shared control. This lack of consensus complicates cross fertilization of...journal article 2018
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Mans, R. (author), Else-Hassing, J. (author), Wijsman, M. (author), Giezekamp, Annabel (author), Pronk, J.T. (author), Daran, J.G. (author), van Maris, A.J.A. (author)CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25...journal article 2017
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Arifvianto, B. (author)Loss of function and impaired life quality as a result of large bone defects remain a serious problem in the society. Basically, the bone tissue has the capability of healing by itself when fractured. However, impaired healing may occur, leading to delayed union or even non-union when a bone segment is excised above a critical size. In recent...doctoral thesis 2017
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Shen, Y. (author), Lindenbergh, R.C. (author), Hofland, Bas (author), Kramer, R. (author)For better understanding how coastal structures with gentle slopes behave during high energy events, a wave attack experiment representing a storm of 3000 waves was performed in a flume facility. Two setups with different steepness of slope were compared under the same conditions. In order to quantify changes in the rock slopes after the wave...conference paper 2017
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Huijgens, H.K.M. (author), van Deursen, A. (author), van Solingen, D.M. (author)Context: In this paper we present a multiple case study on the insights of software organizations into stakeholder satisfaction and (perceived) value of their software projects. Our study is based on the notion that quantifying and qualifying project size, cost, duration, defects, and estimation accuracy needs to be done in relation with...journal article 2017
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Bracher, J.M. (author), de Hulster, A.F. (author), Koster, C.C. (author), van den Broek, M.A. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Biotin prototrophy is a rare, incompletely understood, and industrially relevant characteristic of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-free synthetic medium is extremely slow (specific growth rate [μ] ≈ 0.01...journal article 2017
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Voorendt, M.Z. (author), Vrijling, J.K. (author), Voortman, HG (author)A lack of appropriate guidelines for the design and assessment hampers the development of multifunctional flood defenses like parking garages in quays and houses in dikes. The aim of the present paper, therefore, is to gain insight in the structural performance of multifunctional flood defenses and to provide a tool to evaluate design...conference paper 2017
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Voorendt, M.Z. (author)Multifunctional flood defences are structures that primarily protect land from being covered by water and simultaneously serve other purposes. The present dissertation focuses on the combination of flood protection with functions that are fulfilled by means of buildings and objects, with a high degree of structural integration. This can...doctoral thesis 2017
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Abdel-Ghaffar, Khaled A.S. (author), Weber, J.H. (author)The Cadambe-Mazumdar bound gives a necessary condition for a code to have a certain locality in case of a single erasure in terms of length, dimension, and Hamming distance of the code and of certain shortened codes. The bound has been generalized by Rawat, Mazumdar, and Vishwanath to recover multiple erasures in a cooperative repair scenario....conference paper 2017
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Gorter de Vries, A.R. (author), Pronk, J.T. (author), Daran, J.G. (author)Chromosomal copy number variation (CCNV) plays a key role in evolution and health of eukaryotes. The unicellular yeast Saccharomyces cerevisiae is an important model for studying the generation, physiological impact, and evolutionary significance of CCNV. Fundamental studies of this yeast have contributed to an extensive set of methods for...journal article 2017
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Stive, M.J.F. (author)Uittree redepublic lecture 2017
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Papapetridis, I. (author), van Dijk, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Background: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD<sup>+</sup>-dependent acetate reduction has been shown to improve ethanol yields and...journal article 2017
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Loonstijn, M.A. (author)The Diesel A model has been developed with the idea of looking at overall engine cycle performance in a larger system. As such, the goal has always been to provide an acceptable prediction of the engine performance without sacrificing the computational performance of the model. The simplest form of the Diesel A model has only a single integrator...report 2017
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Ng, N.H.Y. (author)Thermodynamics is one of the main pillars of theoretical physics, and it has a special appeal of having wide applicability to a large variety of different physical systems. However, many assumptions in thermodynamics apply only to systems which are bulk material, i.e. consisting a large number of microscopic classical particles. Due to the...doctoral thesis 2017
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Horikx, M.P. (author)The subject of this academic research thesis is a methodical approach on the complex problem-solving process of structural conceptual design.<br/>For this relatively unexplored problem, an exploratory research is conducted by systematically zooming in from the whole to the part and from coarse to fine. The so explored methodical approach on...doctoral thesis 2017
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Roy, S. (author), Hermans, F.F.J. (author), van Deursen, A. (author)Despite being popular end-user tools, spreadsheets suffer from the vulnerability of error-proneness. In software engineering, testing has been proposed as a way to address errors. It is important therefore to know whether spreadsheet users also test, or how do they test and to what extent, especially since most spreadsheet users do not have...conference paper 2017
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Antolović, I.M. (author), Burri, S. (author), Bruschini, Claudio (author), Hoebe, Ron A. (author), Charbon-Iwasaki-Charbon, E. (author)sCMOS imagers are currently utilized (replacing EMCCD imagers) to increase the acquisition speed in super resolution localization microscopy. Single-photon avalanche diode (SPAD) imagers feature frame rates per bit depth comparable to or higher than sCMOS imagers, while generating microsecond 1-bit-frames without readout noise, thus paving...journal article 2017