Searched for: subject%3A%22Escherichia%255C%2Bcoli%22
(1 - 16 of 16)
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Wehrens, Martijn (author), Krah, Laurens H.J. (author), Towbin, Benjamin D. (author), Hermsen, Rutger (author), Tans, S.J. (author)
The inherent stochasticity of metabolism raises a critical question for understanding homeostasis: are cellular processes regulated in response to internal fluctuations? Here, we show that, in E. coli cells under constant external conditions, catabolic enzyme expression continuously responds to metabolic fluctuations. The underlying...
journal article 2023
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Büke, F. (author), Grilli, Jacopo (author), Cosentino Lagomarsino, Marco (author), Bokinsky, G.E. (author), Tans, S.J. (author)
Growth and division are central to cell size. Bacteria achieve size homeostasis by dividing when growth has added a constant size since birth, termed the adder principle, by unknown mechanisms.<sup>1</sup><sup>,</sup><sup>2</sup> Growth is well known to be regulated by guanosine tetraphosphate (ppGpp), which controls diverse processes from...
journal article 2022
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D'Angelo, Francesca (author), Fernandez Fueyo, E. (author), Garcia, Pierre Simon (author), Shomar Monges, H. (author), Cebola Rebelo Manuel, R.S. (author), Büke, F. (author), van den Broek, N.J.F. (author), de Ram, C. (author), Pabst, Martin (author)
Iron-sulfur (Fe-S) clusters are ancient and ubiquitous protein cofactors and play irreplaceable roles in many metabolic and regulatory processes. Fe-S clusters are built and distributed to Fe-S enzymes by dedicated protein networks. The core components of these networks are widely conserved and highly versatile. However, Fe-S proteins and...
journal article 2022
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Barrios Hernandez, M.L. (author), Bettinelli, Carolina (author), Mora-Cabrera, Karen (author), Vanegas-Camero, Maria Clara (author), Garcia, H. (author), van de Vossenberg, Jack (author), Prats, Daniel (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author)
The aerobic granular sludge (AGS) process is an effective wastewater treatment technology for organic matter and nutrient removal that has been introduced in the market rapidly. Until now, limited information is available on AGS regarding the removal of bacterial and viral pathogenic organisms present in sewage. This study focussed on...
journal article 2021
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Vasilakou, E. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)
Background: Microbial metabolism is highly dependent on the environmental conditions. Especially, the substrate concentration, as well as oxygen availability, determine the metabolic rates. In large-scale bioreactors, microorganisms encounter dynamic conditions in substrate and oxygen availability (mixing limitations), which influence their...
journal article 2020
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Imholz, N.C.E. (author), Noga, M.J. (author), van den Broek, N.J.F. (author), Bokinsky, Gregory (author)
The molecule guanosine tetraphophosphate (ppGpp) is most commonly considered an alarmone produced during acute stress. However, ppGpp is also present at low concentrations during steady-state growth. Whether ppGpp controls the same cellular targets at both low and high concentrations remains an open question and is vital for understanding...
journal article 2020
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Lehner, B. (author), Schmieden, D.T. (author), Meyer, A.S. (author)
Sustainable and personally tailored materials production is an emerging challenge to society. Living organisms can produce and pattern an extraordinarily wide range of different molecules in a sustainable way. These natural systems offer an abundant source of inspiration for the development of new environmentally friendly materials production...
journal article 2017
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Velasco Alvarez, M.I. (author), ten Pierick, A. (author), van Dam, P.T.N. (author), Maleki Seifar, R. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)
Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale...
journal article 2017
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Anderson, C. (author), Malambo, D.H. (author), Gonzalez Perez, M.E. (author), Nobela, H.N. (author), De Pooter, L. (author), Spit, J. (author), Hooijmans, C.M. (author), Van de Vossenberg, J. (author), Greya, W. (author), Thole, B. (author), Van Lier, J.B. (author), Brdjanovic, D. (author)
In this research, three faecal sludge sanitizing methods—lactic acid fermentation, urea treatment and lime treatment—were studied for application in emergency situations. These methods were investigated by undertaking small scale field trials with pit latrine sludge in Blantyre, Malawi. Hydrated lime was able to reduce the E. coli count in the...
journal article 2015
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Krishnan, S.T. (author), Moolman, M.C. (author), Van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)
Background Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date, ?...
journal article 2015
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Tiruvadi Krishnan, S. (author), Moolman, M.C. (author), van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)
Background: Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date,...
journal article 2015
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Moolman, M.C. (author), Huang, Z. (author), Krishnan, S.T. (author), Kerssemakers, J.W.J. (author), Dekker, N.H. (author)
Background: Controlled restriction of cellular movement using microfluidics allows one to study individual cells to gain insight into aspects of their physiology and behaviour. For example, the use of micron-sized growth channels that confine individual Escherichia coli has yielded novel insights into cell growth and death. To extend this...
journal article 2013
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Lutterodt, G. (author)
The deviation of bacteria transport and deposition patterns on grains in porous media from theory has resulted in the inability to accurately predict transport distances in aquifers, with consequences of polluting drinking water sources (springs, boreholes and wells). Due to the importance of Escherichia coli (E. coli) as an indicator of faecal...
doctoral thesis 2012
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Taymaz Nikerel, H. (author)
Kinetic models, which predict the behaviour of metabolic reaction networks under different conditions, are indispensible to fully and quantitatively understand the relation between a product pathway and connected central metabolism. In this thesis the focus was to develop tools for future in vivo kinetic modeling in Escherichia coli. The...
doctoral thesis 2010
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Cuellar Soares, M.C. (author)
Industrial biotechnology can be defined as the utilization of living organisms or their enzymes for the industrial production of food, beverages, commodity chemicals and pharmaceuticals, among others. A biotechnological production process, so-called bioprocess, mostly comprises product formation (e.g. by fermentation) and product recovery and...
doctoral thesis 2008
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De Boer, A.P.N. (author), Reijnders, W.N.M. (author), Kuenen, J.G. (author), Stouthamer, A.H. (author), Van Spanning, R.J.M. (author)
journal article 1994
Searched for: subject%3A%22Escherichia%255C%2Bcoli%22
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