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Turnover of the extracellular polymeric matrix of granules performing biological phosphate removal

Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)

Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...

journal article 2023

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Review of the action of organic matter on mineral sediment flocculation

Deng, Zhirui (author), Huang, Dong (author), He, Qing (author), Chassagne, C. (author)

Sediment is found throughout the world’s alluvial plain rivers, estuarine coasts and adjacent seas and is thereby a key factor in major ecosystems. Suspended mineral sediment can affect the biological activity of microorganisms and plants, by reducing light penetration in the water column or by binding to organic matter. Biological processes...

review 2022

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Contribution of extracellular polymeric substances fractions to the adsorption of silver nanoparticles by activated sludge

Xu, Yanghui (author), Ou, Qin (author), He, Qiang (author), Wu, Zhengsong (author), Ma, Jun (author), Huangfu, Xiaoliu (author)

The extracellular polymeric substances (EPS) from activated sludge played significant roles in the removal of nanoparticles from wastewater. A series of batch experiments were carried out to determine the adsorption mechanism of three nano-Ag by activated sludge, as well as the contributions of EPS fractions including dissolved EPS (DEPS),...

journal article 2022

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Catabolism of sialic acids in an environmental microbial community

Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...

journal article 2022

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Insight on how biopolymers recovered from aerobic granular wastewater sludge can reduce the flammability of synthetic polymers

Kim, Nam Kyeun (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Eco-friendly flame retardants are greatly required to meet the expectations of low-toxicity, environmental compatibility and sustainability. Extracellular polymeric substances (EPS), the biopolymers recovered from excess granular wastewater sludge, have been successfully incorporated into poly(vinyl alcohol) (PVA) by a solution casting method...

journal article 2022

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Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”

Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...

journal article 2021

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Formation and ripening of alginate-like exopolymer gel layers during and after membrane filtration

Pfaff, N.M. (author), Kleijn, J. Mieke (author), van Loosdrecht, Mark C.M. (author), Kemperman, Antoine J.B. (author)

The properties of biofilm EPS are determined by the multiple interactions between its constituents and the surrounding environment. Because of the high complexity of biofilm EPS, its constituents' characterisation is still far from thorough, and identification of these interactions cannot be done yet. Therefore, we use gels of bacterial...

journal article 2021

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Use of near-infrared spectroscopy on predicting wastewater constituents to facilitate the operation of a membrane bioreactor

Kim, E. (author), Ćurko, Josip (author), Gajdoš Kljusurić, Jasenka (author), Matošić, Marin (author), Crnek, Vlado (author), López-Vázquez, Carlos M. (author), Garcia, H. (author), Brdjanovic, Damir (author), Valinger, Davor (author)

The use of near-infrared (NIR) spectroscopy in wastewater treatment has continuously expanded. As an alternative to conventional analytical methods for monitoring constituents in wastewater treatment processes, the use of NIR spectroscopy is considered to be cost-effective and less time-consuming. NIR spectroscopy does not distort the...

journal article 2021

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Assessment of the impact of temperature on biofilm composition with a laboratory heat exchanger module

Pinel, I.S.M. (author), Biskauskaite, R. (author), Palova, E. (author), Vrouwenvelder, J.S. (author), van Loosdrecht, Mark C.M. (author)

Temperature change over the length of heat exchangers might be an important factor affect-ing biofouling. This research aimed at assessing the impact of temperature on biofilm accumulation and composition with respect to bacterial community and extracellular polymeric substances. Two identical laboratory-scale plate heat exchanger modules...

journal article 2021

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Aerobic granular sludge contains Hyaluronic acid-like and sulfated glycosaminoglycans-like polymers

Felz, S. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...

journal article 2020

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Flame retardant property of flax fabrics coated by extracellular polymeric substances recovered from both activated sludge and aerobic granular sludge

Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

In this research, extracellular polymeric substances (EPS), such as EPS_flocs and EPS_granules, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...

journal article 2020

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Impact of metal ions on structural EPS hydrogels from aerobic granular sludge

Felz, S. (author), Kleikamp, H.B.C. (author), Zlopasa, J. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Structural extracellular polymeric substances (structural EPS) can form stable hydrogels and are considered to be responsible for the stability of biofilms and aerobic granular sludge. Structural EPS were extracted from aerobic granular sludge and characterized for their gel-forming capacity with different alkaline earth and transition metal...

journal article 2020

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A comparison between chemical cleaning efficiency in lab-scale and full-scale reverse osmosis membranes: Role of extracellular polymeric substances (EPS)

Jafari Eshlaghi, M. (author), D'haese, A. (author), Zlopasa, J. (author), Cornelissen, E. R. (author), Vrouwenvelder, J.S. (author), Verbeken, K. (author), Verliefde, A. (author), van Loosdrecht, Mark C.M. (author), Picioreanu, C. (author)

Chemical cleaning is vital for the optimal operation of membrane systems. Membrane chemical cleaning protocols are often developed in the laboratory flow cells (e.g., Membrane Fouling Simulator (MFS)) using synthetic feed water (nutrient excess) and short experimental time of typically days. However, full-scale Reverse Osmosis (RO) membranes...

journal article 2020

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Structural extracellular polymeric substances determine the difference in digestibility between waste activated sludge and aerobic granules

Guo, H. (author), Felz, S. (author), Lin, Y. (author), van Lier, J.B. (author), de Kreuk, M.K. (author)

Aerobic granular sludge (AGS) technology is an alternative to conventional activated sludge to reduce the process footprint and energy consumption. Strategies for the efficient management of its produced biomass, that is grown in a granular morphology as well, need further development. Anaerobic digestion (AD) is commonly applied in waste...

journal article 2020

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Towards enhanced second-generation n-butanol production from sugarcane

Zetty Arenas, A.M. (author)

Nowadays, the biotechnology industry is facing the challenge of producing suitable equivalents for petroleum-based products from renewable resources in a sustainable and economically feasible way. Finding cleaner alternatives for gasoline, fuels, and chemicals, has been the subject of research worldwide, whether for economic, geopolitical, or...

doctoral thesis 2019

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The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803

Pereira, Sara B. (author), Santos, Marina (author), Leite, José P. (author), Flores, Carlos (author), Eisfeld, C. (author), Büttel, Zsófia (author), Mota, Rita (author), Rossi, Federico (author), De Philippis, Roberto (author)

Many cyanobacteria produce extracellular polymeric substances (EPS) mainly composed of heteropolysaccharides with unique characteristics that make them suitable for biotechnological applications. However, manipulation/optimization of EPS biosynthesis/characteristics is hindered by a poor understanding of the production pathways and the...

journal article 2019

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Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)

Felz, S. (author), Vermeulen, Pascalle (author), van Loosdrecht, Mark C.M. (author), Lin, Yue Mei (author)

Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on...

journal article 2019

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Modelling the influence of physical properties of extracellular polymeric substances in aerobic granule morphology

de Bruin, Stefan (author)

This thesis examines the influence of physical properties of structural EPS on granule morphology. Two types of structural EPS are found to be important. These types of structural EPS are: surface bound EPS and hydrogel forming EPS matrix. VirtualLeaf is used to model granules which contain only binding surface bound EPS. The TST model is used...

master thesis 2018

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Variation of Chemical and Structural properties of Waste Aerobic Granular Sludge (WAGS) and Flocculent Sludge in Anaerobic Digestion

Fang, Jiaxing (author)

A comparative batch experiment as well as a biochemical methane potential(BMP) test were conducted over 44d for aerobic granular sludge (WAGS) and waste flocculent activated sludge(WAS) on sludge anaerobic digestion (AD), to give an insight into the chemical and mechanical properties change of the EPS in different sludge source during AD process...

student report 2018

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Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria

Lin, Y. (author), Reino, Clara (author), Carrera, Julián (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)

A new type of structural extracellular polymers (EPS) was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and FTIR spectroscopy to characterize specific amino acids and protein secondary structure, and by SDS-PAGE with different stains to identify different glycoconjugates. Its...

journal article 2018