Searched for: subject%3A%22Fluorescence%255C+imaging%22
(1 - 17 of 17)
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Spengler, Daniel (author)
The study of tumor microenvironments (TMEs) and immune cell composition in cancer, a disease characterized by uncontrolled growth and spread of tumor cells, has become increasingly important for understanding tumor progression and patient outcomes. Tools such as the TME-Analyzer enable this kind of research, but their manual workflows highlight...
master thesis 2023
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Baumgartner, L.M. (author), Kahn, A.P. (author), Hoogland, Maxime (author), Bleeker, J.J. (author), Jager, W.F. (author), Vermaas, D.A. (author)
Electrochemical CO<sub>2</sub> reduction poses a promising pathway to produce hydrocarbon chemicals and fuels without relying on fossil fuels. Gas diffusion electrodes allow high selectivity for desired carbon products at high current density by ensuring a sufficient CO<sub>2</sub> mass transfer rate to the catalyst layer. In addition to CO...
journal article 2023
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Lok, Pieter (author)
The performance of hydrostatic bearings is in part decided by their lubrication-film height shape. Imaging the shape can be done by dissolving fluorescent dyes in the layer. However, current imaging techniques are rooted in heuristics, and as such, their physics, design parameters, and error sources are not quantitively understood. To fully...
master thesis 2022
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Filius, M. (author)
Proteins are the workhorses of the cell, as such, they form the basis of all living systems. In order to fully understand biological processes, the ability to identify and quantify the proteins in cells is crucial. Identification can be achieved by determining the amino acid sequence of proteins, since this sequence is unique for each protein....
doctoral thesis 2022
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Holub, M. (author), Birnie, A.T.F. (author), Japaridze, A. (author), van der Torre, J. (author), Ridder, Maxime den (author), de Ram, C. (author), Pabst, Martin (author), Dekker, C. (author)
Chromosome structure and function is studied using various cell-based methods as well as with a range of in vitro single-molecule techniques on short DNA substrates. Here, we present a method to obtain megabase-pair-length deproteinated DNA for in vitro studies. We isolated chromosomes from bacterial cells and enzymatically digested the...
journal article 2022
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Dijkhuis, Tom (author)
Introduction Near-infrared fluorescence (NIRF) imaging is a relatively novel development in colorectal liver metastases (CRLM) surgery which has proven to be an added value for the detection of new lesions and surgical guidance. However, new possibilities of NIRF imaging in CRLM surgery are yet to be explored. The fluorescent rim of indocyanine...
master thesis 2021
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van Loon, A. (author), Noble, P. (author), de Man, D. (author), Alfeld, M.W.E.M. (author), Dore-Callewaert, T.W.J. (author), van der Snickt, G.R. (author), Janssens, K.E.J. (author), Dik, J. (author)
As part of the NWO Science4Arts REVISRembrandt project (2012–2018), novel chemical imaging techniques were developed and applied to the study of Rembrandt’s late experimental painting technique (1651–1669). One of the unique features in his late paintings is his abundant use of smalt: a blue cobalt glass pigment that he often combined with...
journal article 2020
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van Beek, Gregory (author)
In budding yeast, a certain concentration of the GTPase Cdc42 is optimal for cell division. This optimal concentration depends on the phenotype and genetic background of the cells. Due to bet hedging, the Cdc42 concentration is different between cells in a population and it is expected that the concentration, measured for many cells, is gamma...
master thesis 2019
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Vandivere, Abbie (author), van Loon, A. (author), Dore-Callewaert, T.W.J. (author), Haswell, Ralph (author), Proaño Gaibor, Art Ness (author), van Keulen, Henk (author), Leonhardt, Emilien (author), Dik, J. (author)
The background of Vermeer’s Girl with a Pearl Earring (c. 1665, Mauritshuis) has, until recently, been interpreted as a flat dark space. The painting was examined in 2018 as part of the research project The Girl in the Spotlight using a combination of micro- and macro-scale analytical techniques. The stratigraphy of the background was...
journal article 2019
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MacLennan, Douglas (author), Trentelman, Karen (author), Szafran, Yvonne (author), Woollett, Anne T. (author), Delaney, John K. (author), Janssens, Koen (author), Dik, J. (author)
Over the past several decades the painting An Old Man in Military Costume by Rembrandt Harmensz van Rijn (ca. 1630–31; J. Paul Getty Museum, 78.PB.246) has been the subject of a number of investigations carried out in order to better visualize a second painting beneath the surface figure. The underlying image–the head and shoulders of a man...
journal article 2019
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Warman, J.M. (author), de Haas, M.P. (author), Luthjens, L.H. (author), Yao, T. (author), Navarro-Campos, Julia (author), Yuksel, S. (author), Aarts, J.M. (author), Thiele, Simon (author), Houter, Jacco (author), in het Zandt, Wilco (author)
Radio-fluorogenic (RFG) gels become permanently fluorescent when exposed to high-energy radiation with the intensity of the emission proportional to the local dose of radiation absorbed. An apparatus is described, FluoroTome 1, that is capable of taking a series of tomographic images (thin slices) of the fluorescence of such an irradiated RFG...
journal article 2019
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Antolović, I.M. (author)
The aim of this research is to explore the potential advantages of SPAD imagers used in microscopy. An ideal microscopy detector requires high sensitivity (high quantum efficiency QE or photon detection probability PDP), photon counting operation, low noise (dark current or dark count rate), timing resolution in the order of 100 ps, frame rate...
doctoral thesis 2018
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van der Graaff, L. (author), Boyaval, Fanny (author), van Vliet, L.J. (author), Stallinga, S. (author)
In the field of pathology there is an ongoing transition to the use of Whole Slide Imaging (WSI) systems which scan tissue slides at intermediate resolution (0∼.25 μm) and high throughput (15mm<sup>2</sup>=min) to digital image files. Most scanners currently on the market are line-sensor based push-broom scanners for three-color (RGB)...
conference paper 2018
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Dooley, Kathryn A. (author), Gifford, E. Melanie (author), van Loon, A. (author), Noble, Petria (author), Zeibel, Jason G. (author), Conover, Damon M. (author), Alfeld, Matthias (author), van der Snickt, G.R. (author), Legrand, Stijn (author), Janssens, Koen (author), Dik, J. (author), Delaney, John K. (author)
Late paintings of Rembrandt van Rijn (1606–1669) offer intriguing problems for both art historians and conservation scientists. In the research presented here, the key question addressed is whether observed stylistic differences in paint handling can be correlated with material differences. In Saul and David, in the collection of the Royal...
journal article 2018
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Haring, M.T. (author), Liv Hamarat, N. (author), Zonnevylle, A.C. (author), Narvaez Gonzalez, A.C. (author), Voortman, L.M. (author), Kruit, P. (author), Hoogenboom, J.P. (author)
In the biological sciences, data from fluorescence and electron microscopy is correlated to allow fluorescence biomolecule identification within the cellular ultrastructure and/or ultrastructural analysis following live-cell imaging. High-accuracy (sub-100 nm) image overlay requires the addition of fiducial markers, which makes overlay accuracy...
journal article 2017
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average number of localizations per fluorophore, or...
journal article 2015
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Van Oosterom, M.N. (author), Kreuger, R. (author), Buckle, T. (author), Mahn, W.A. (author), Bunschoten, A. (author), Josephson, L. (author), Van Leeuwen, F.W.B. (author), Beekman, F.J. (author)
Background: In vivo bioluminescence, fluorescence, and single-photon emission computed tomography (SPECT) imaging provide complementary information about biological processes. However, to date these signatures are evaluated separately on individual preclinical systems. In this paper, we introduce a fully integrated bioluminescence-fluorescence...
journal article 2014
Searched for: subject%3A%22Fluorescence%255C+imaging%22
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