Searched for: subject%3A%22Hydroxylation%22
(1 - 12 of 12)
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Gonzales, A. (author), van Lier, J.B. (author), de Kreuk, M.K. (author)
The pre-treatment of waste activated sludge (WAS) has become more common since it often results in improved bioconversion to methane, in both rate and extent. However, thorough insights on the possible effects and mechanisms of mild pre-treatment techniques, such as temperatures &lt;100 °C combined with the addition of H<sub>2</sub>O<sub>2<...
journal article 2022
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Suresh Babu, Diwakar (author), Mol, J.M.C. (author), Buijnsters, J.G. (author)
GenX is the trade name of the ammonium salt of hexafluoropropylene oxide dimer acid (HFPO-DA) and is used as a replacement for the banned perfluorooctanoic acid (PFOA). However, recent studies have found GenX to be more toxic than PFOA. This work deals with the electrochemical degradation of HFPO-DA using boron-doped diamond anodes. For the...
journal article 2022
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Zhang, Jie (author), Tan, Lirong (author), Hagedoorn, P.L. (author), Wang, Ruiqi (author), Wen, Li (author), Wu, Siwei (author), Tan, Xuemei (author), Xu, Hui (author), Zhou, Xing (author)
Bisphenols are important industrial materials for example for the production of plastics, but are also well known for their adverse health effects, in particular bisphenol A (BPA) is an endocrine disruptor. The widespread use of plastics has raised concerns. Therefore, the removal of bisphenols from wastewater has sparked the interest of the...
journal article 2022
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Suresh Babu, Diwakar (author)
The presence of harmful pollutants and toxic pathogens in water is a risk to both living beings and the environment. Water treatment plays a crucial role in the removal of these contaminants through different stages of filtration. Among the existing pollutants, a family of per-and polyfluoroalkyl substances (PFAS) escapes from all treatment...
master thesis 2021
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Tonin, F. (author), Tieves, F. (author), Willot, S.J. (author), Van Troost, Anouska (author), van Oosten, R.J.C. (author), Breestraat, Stefaan (author), Van Pelt, Sander (author), Alcalde, Miguel (author), Hollmann, F. (author)
The pilot-scale production of the peroxygenase from Agrocybe aegerita (rAaeUPO) is demonstrated. In a fed-batch fermentation of the recombinant Pichia pastoris, the enzyme was secreted into the culture medium to a final concentration of 0.29 g L-1 corresponding to 735 g of the peroxygenase in 2500 L of the fermentation broth after 6 days. Due...
journal article 2021
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Paul, C.E. (author), Eggerichs, Daniel (author), Westphal, Adrie H. (author), Tischler, Dirk (author), van Berkel, Willem J.H. (author)
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set of chemo-, regio- and enantioselective oxyfunctionalization reactions. In this review, we describe how FPMOs have evolved from model enzymes in mechanistic flavoprotein research to biotechnologically relevant catalysts that can be applied for...
review 2021
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Hobisch, Markus (author), van Schie, M.M.C.H. (author), Kim, Jinhyun (author), Røjkjær Andersen, Kasper (author), Alcalde, Miguel (author), Kourist, Robert (author), Park, Chan Beum (author), Hollmann, F. (author), Kara, S. (author)
The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H<sub>2</sub>O<sub>2</sub> was photocatalytically generated in...
journal article 2020
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Perz, Frederic (author), Bormann, Sebastian (author), Ulber, Roland (author), Alcalde, Miguel (author), Bubenheim, Paul (author), Hollmann, F. (author), Holtmann, Dirk (author), Liese, Andreas (author)
Unspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we...
journal article 2020
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Yuan, Bo (author), Mahor, Durga (author), Fei, Qiang (author), Wever, Ron (author), Alcalde, Miguel (author), Zhang, Wuyuan (author), Hollmann, F. (author)
Peroxyzymes simply use H2O2 as a cosubstrate to oxidize a broad range of inert C-H bonds. The lability of many peroxyzymes against H2O2 can be addressed by a controlled supply of H2O2, ideally in situ. Here, we report a simple, robust, and water-soluble anthraquinone sulfonate (SAS) as a promising organophotocatalyst to drive both...
journal article 2020
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Smit, Stefan (author)
Osteogenesis imperfecta (OI), also known as brittle bone disease, affects 1 in 10.000 births. This disease is characterized by skeletal dysplasias, bone fragility and many secondary health issues. It is a heritable disorder affecting collagen type I, which is an essential protein in bone tissue. Post-translational overmodifications in collagen...
master thesis 2018
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Fernandez Fueyo, E. (author), Ni, Y. (author), Gomez Baraibar, A. (author), Alcalde, Miguel (author), van Langen, L.M. (author), Hollmann, F. (author)
The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and <sup>tert</sup...
journal article 2016
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Cerezo Palacios, J.M. (author)
In this PhD work, a new surface treatment based on the application of Zr-based conversion coatings by immersion in a Cu containing Zr-based conversion solution was investigated as a replacement of the traditional phosphating process for the automotive industry. Nowadays most of the cars are made of a combination of different metals, therefore,...
doctoral thesis 2015
Searched for: subject%3A%22Hydroxylation%22
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