Cell Tracking for Organoids: Lessons From Developmental Biology

Organoids have emerged as powerful model systems to study organ development and regeneration at the cellular level. Recently developed microscopy techniques that track individual cells through space and time hold great promise to elucidate the organizational principles of organs and organoids. Applied extensively in the past decade to embryo...

An integrated measurement approach for the determination of the aerodynamic loads and structural motion for unsteady airfoils

The structural motion and unsteady aerodynamic loads of a pitching airfoil model that features an actuated trailing edge flap are determined experimentally using a single measurement and data processing system. This integrated approach provides an alternative to the coordinated use of multiple measurement systems for simultaneous position and...

The Human Body Model versus conventional gait models for kinematic gait analysis in children with cerebral palsy

With the rise of biofeedback in gait training in cerebral palsy there is a need for real-time measurements of gait kinematics. The Human Body Model (HBM) is a recently developed model, optimized for the real-time computing of kinematics. This study evaluated differences between HBM and two commonly used models for clinical gait analysis: the...

Machinic Magic

This project is an attempt to bring back play, from the mere simulation it is now, to a creative force that it once was. It questions whether the entertainment park is still relevant in contemporary society and whether it can be reimagined and transformed from a scripted to a more unscripted arena of action. The aim of this project is to create...

Novel method of alignment to buried cavities in cavity-SOI wafers for advanced MEMS devices

Accurate alignment between the cavities in cavity-SOI (c-SOI) wafers and lithography on the wafer surface is essential to advanced MEMS production. Existing alignment methods are well defined, but often require specialized equipment or costly software packages available only in professional manufacturing environments. It would be beneficial...

Phenotype-independent isolation of interspecies Saccharomyces hybrids by dual-dye fluorescent staining and fluorescence-activated cell sorting

Interspecies hybrids of Saccharomyces species are found in a variety of industrial environments and often outperform their parental strains in industrial fermentation processes. Interspecies hybridization is therefore increasingly considered as an approach for improvement and diversification of yeast strains for industrial application....

Precision of determining bone pose and marker position in the foot and lower leg from computed tomography scans: How low can we go in radiation dose?

Computed tomography (CT) imaging can be used to determine bone pose, sometimes combined with skin-mounted markers. For this specific application, a lower radiation dose than the conventional clinical dose might suffice. This study aims to determine how lowering the radiation dose of a CT-scan of the ankle and foot affects the precision of...

Effects of surgical flow disruptions on surgeons’ resources: a pilot study

Background: Minimally invasive surgery requires surgeons to allocate more attention and efforts than open surgery. A surgeon’s pool of resource is affected by the multiple occurrences of interruptions and distractions in the operating room. Surgical flow disruption has been addressed from a quantitative perspective. However, little is known...

Desiccation, Crust Formation & Consolidation of Soft, Cohesive Soil due to Atmospheric Conditions: A case study of the Marker Wadden

Deterioration of the ecological quality within the Markermeer, due to the elimination of natural flushing and a resulting increase in turbidity, has created a need to revitalize the region for the benefit of the local wildlife. One major way in which this is being achieved is through the Marker Wadden, designed as a wetland area featuring a...

Characterization of pathogenic SORL1 genetic variants for association with Alzheimer's disease: A clinical interpretation strategy

Accumulating evidence suggests that genetic variants in the SORL1 gene are associated with Alzheimer disease (AD), but a strategy to identify which variants are pathogenic is lacking. In a discovery sample of 115 SORL1 variants detected in 1908 Dutch AD cases and controls, we identified the variant characteristics associated with SORL1...

The effect of initial concentration on the consolidation behaviour of mud: A study on lake Markermeer sediment

Land reclamations in deltaric areas provide space to meet the demand for a safe place to live and work. Sand, a traditional filling material in some locations is becoming scarcer. An alternative filling material is mud. Mud consists of clay, silt, sand, organic material, water and gas. Therefore, it is a versatile material and difficult to...

Visitor center Marker Wadden

Het Bezoekerscentrum Marker Wadden is het verbindende element tussen de beschermde en gedefinieerde karaktereigenschappen van de gebouwde omgeving, en de grilligheid en onvoorspelbaarheid van de nieuwe natuur. Het centrum huisvest een tentoonstellingsruimte, restaurant, uitkijktoren, lodges en hun ondersteunende functies. De bezoeker arriveert...

Metagenomic analysis of nitrogen and methane cycling in the Arabian Sea oxygen minimum zone

Oxygen minimum zones (OMZ) are areas in the global ocean where oxygen concentrations drop to below one percent. Low oxygen concentrations allow alternative respiration with nitrate and nitrite as electron acceptor to become prevalent in these areas, making them main contributors to oceanic nitrogen loss. The contribution of anammox and...

Enhancing re-detection efficacy of defects on blank wafers using stealth fiducial markers

To qualify tools of semiconductor manufacturing, particles unintentionally deposited in these tools are character-ized using blank wafers. With fast optical inspection tools one can quickly localize these particle defects. An ex-ample is TNO's Rapid Nano, which operates in optical darkfield. The next step is defect review for further...

Stealth fiducial markers: Enhancing re-detection efficacy of defects on blank wafers

In this thesis a stealth fiducial marker system for blank wafers is designed, fabricated and validated. The goal of this marker system it to map the coordinates of TNO’s Rapid Nano (a fast optical inspection tool) to the coordinates of scanning electron microscopes and atomic force microscopes. This way defects, that have been detected in the...

Reducing the genetic complexity of glycolysis in Saccharomyces cerevisiae

Glycolysis, a biochemical pathway that oxidizes glucose to pyruvate, is at the core of sugar metabolism in Saccharomyces cerevisiae (bakers’ yeast). Glycolysis is not only a catabolic route involved in energy conservation, but also provides building blocks for anabolism. From an applied perspective, several glycolytic intermediates are key...

An integrated approach of gene expression and DNA-methylation profiles of WNT signaling genes uncovers novel prognostic markers in Acute Myeloid Leukemia

Background The wingless-Int (WNT) pathway has an essential role in cell regulation of hematopoietic stem cells (HSC). For Acute Myeloid Leukemia (AML), the malignant counterpart of HSC, currently only a selective number of genes of the WNT pathway are analyzed by using either gene expression or DNA-methylation profiles for the identification of...

amdSYM, a new dominant recyclable marker cassette for Saccharomyces cerevisiae

Despite the large collection of selectable marker genes available for Saccharomyces cerevisiae, marker availability can still present a hurdle when dozens of genetic manipulations are required. Recyclable markers, counterselectable cassettes that can be removed from the targeted genome after use, are therefore valuable assets in ambitious...