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Neves Sao Pedro, M. (author)Continuous biomanufacturing is considered the future phase for the optimization of production processes in the biopharmaceutical industry. Productivity, product quality and consistency are greatly improved while production costs and environmental footprint are drastically reduced. The manufacturing of monoclonal antibodies (mAbs), an important...doctoral thesis 2023
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Tjokro, Kristie (author)In microfluidics, typical laboratory processes can be condensed to a miniature device. This reduces analysis time and required volumes of samples and reagents, increases mobility and flexibility, and is cost effective. In this work, the environmental impacts of three microfluidic devices for glucose detection are assessed using a comparative...master thesis 2023
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Dubbelman, Thomas (author)Crystallization is one of the most common separation techniques in chemical industry. Nonetheless, the nucleation step in the crystallization process is little understood. This makes it difficult to gain control over the crystallization process and more specifically crystal properties. Nucleation mechanics is therefore studied thoroughly. Non...master thesis 2023
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Motreuil-Ragot, P.A. (author)An organ-on-chip (OoC) is a microelectromechanical (MEMS) device that aims to recapitulate in-vitro the physiology of the smallest functional unit of an organ in order to perform drug analysis or study disease models. OoCs are very complex systems that require actuation and sensing capabilities within controlled and delicate environment. The...doctoral thesis 2023
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Struijk, Victor (author)Life at low-Reynolds numbers regime is intriguing. Single motile bacteria are known to exhibit erratic behaviour; they swim in what is known as a random walk, alternating between periods of swimming straight and abrupt moments of re-orientation. Yet in dense populations, cells of E.coli have been reported to exhibit weak synchronization and self...master thesis 2023
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Braem, Sarah (author)Drug efficacy and side effects are significantly impacted by the first-pass metabolism, a sequence of events that occurs in the stomach and the liver after oral ingestion. Accurate prediction of this mechanism is crucial for a faster and more cost-efficient drug development process. Replicating the first-pass metabolism in vitro using standard...master thesis 2023
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Tsan, Vi hung (author), Fan, D. (author), Caneva, S. (author), Smith, C.S. (author), Verbiest, G.J. (author)A low-cost glass-based microfluidic flow cell with a piezo actuator is built using off-the-shelf parts (total cost €9 per device) to apply acoustophoretic force on polystyrene micro-beads. The main challenge in the fabrication of these devices was to ensure their leak tightness, which we solved using double-sided tape and nail polish. Beads...journal article 2023
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Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregatesNeves Sao Pedro, M. (author), Eppink, Michel H.M. (author), Ottens, M. (author)The lack of process analytical technologies able to provide real-time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is a major critical quality attribute (CQA) with several known process...journal article 2023
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Trapp, S. (author), Lammers, Tom (author), Engudar, Gokce (author), Hoehr, Cornelia (author), Denkova, A.G. (author), Paulssen, E. (author), de Kruijff, R.M. (author)Background: The radionuclide Ga-68 is commonly used in nuclear medicine, specifically in positron emission tomography (PET). Recently, the interest in producing Ga-68 by cyclotron irradiation of [<sup>68</sup>Zn]Zn nitrate liquid targets is increasing. However, current purification methods of Ga-68 from the target solution consist of multi...journal article 2023
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Blöbaum, Luisa (author), Haringa, C. (author), Grünberger, Alexander (author)Bioprocesses are scaled up for the production of large product quantities. With larger fermenter volumes, mixing becomes increasingly inefficient and environmental gradients get more prominent than in smaller scales. Environmental gradients have an impact on the microorganism's metabolism, which makes the prediction of large-scale performance...review 2023
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Bazyar, H. (author)Microfluidic technology is a powerful tool to enable the rapid, accurate, and on-site analysis of forensically relevant evidence on a crime scene. This review paper provides a summary on the application of this technology in various forensic investigation fields spanning from forensic serology and human identification to discriminating and...review 2023
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Neves Sao Pedro, M. (author), Isaksson, Madelène (author), Gomis-Fons, Joaquín (author), Eppink, Michel H.M. (author), Nilsson, Bernt (author), Ottens, M. (author)The implementation of continuous processing in the biopharmaceutical industry is hindered by the scarcity of process analytical technologies (PAT). To monitor and control a continuous process, PAT tools will be crucial to measure real-time product quality attributes such as protein aggregation. Miniaturizing these analytical techniques can...journal article 2023
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Nagalingam, Nagaraj (author), Raghunathan, Aswin (author), Korede, V.B. (author), Overmars, E.F.J. (author), Hung, S. (author), Hartkamp, Remco (author), Padding, J.T. (author), Smith, C.S. (author), Eral, H.B. (author)Optofluidic devices have revolutionized the manipulation and transportation of fluid at smaller length scales ranging from micrometers to millimeters. We describe a dedicated optical setup for studying laser-induced cavitation inside a microchannel. In a typical experiment, we use a tightly focused laser beam to locally evaporate the solution...journal article 2023
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Kurniawan, Tetuko (author), Sahebdivani, Mahsa (author), Zaremba, Damian (author), Blonski, Slawomir (author), Garstecki, Piotr (author), van Steijn, V. (author), Korczyk, Piotr M. (author)Two decades of research on droplet formation in microchannels have led to the widely accepted view that droplets form through the squeezing mechanism when interfacial forces dominate over viscous forces. The initially surprising finding that the volume of the droplets is insensitive to the relative importance of these two forces is nowadays...journal article 2023
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Muralidharan, A. (author)Delivery of biomolecules using pulsed electric fields or electrotransfer has applications such as biomedical engineering, bioprocess engineering and genomic engineering. When a cell is placed in an electric field, the induced transmembrane voltage catalyzes the formation of pores on the cell membrane. This enables the delivery of otherwise cell...doctoral thesis 2022
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Angenent, Maricke (author)Cardiorenal Syndrome (CRS) is a multi-organ disease characterized by a reciprocal pathological interaction between the heart and kidneys during acute or chronic injury. Yet, a complete understanding of the underlying bi-directional pathophysiological mechanisms is lacking, hence obscuring effective diagnostic strategies, patient stratification...master thesis 2022
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Tsan, Vi-hung (author)Acoustic Force Spectroscopy (AFS) is a versatile tool that uses sound waves to manipulate tiny particles such as cells, bacteria, and even zebrafish embryos in microfluidic systems. This kind of acoustic tweezer is gaining increasing attention due to its high throughput capability and non-invasiveness. In addition, this device allows for...master thesis 2022
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Bekkema, Bart (author)Radiometals re-emerge as a promising alternative in nuclear imaging. Cyclotron production of such radiometals using liquid targets solve critical impurity problems seen with current conventional production methods. Purification employing liquid-liquid extraction in microfluidic environments has recently been investigated. Fluid behaviour in...master thesis 2022
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Raghunathan, Aswin (author)Crystallization is one of the most sought after separation and purification technique in the process industries. However, the fundamental understanding on the formation of crystals poses quite a challenge due to the inherent complexity and stochasticity associated with the process. In the past, several attempts put forth to control crystal...master thesis 2022
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Kislaya, A. (author)The precise manipulation of particles and droplets is crucial to many microfluidic applications in engineering. The design of microfluidic devices is generally tailored to perform a specific task, with each specific application requiring a unique and fixed design. In this way, using a single device to perform multiple analyses of a wide range of...doctoral thesis 2022