Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain

This study explores the relation between biomass-specific succinic acid (SA) production rate and specific growth rate of an engineered industrial strain of Saccharomyces cerevisiae, with the aim to investigate the extent to which growth and product formation can be uncoupled. Ammonium-limited aerobic chemostat and retentostat cultures were...

Uncoupling yeast growth and product formation in chemostat and retentostat cultures

The progress of modern biotechnology has enabled the development of fermentation processes for the production of fuels and chemicals from renewable feedstocks. The current fermentation processes for bio-based production commonly start with a growth phase of the microorganisms followed by a production phase. This implies that biomass formation...

Quantitative Physiology of Non-Energy-Limited Retentostat Cultures of Saccharomyces cerevisiae at Near-Zero Specific Growth Rates

So far, the physiology of Saccharomyces cerevisiae at near-zero growth rates has been studied in retentostat cultures with a growth-limiting supply of the carbon and energy source. Despite its relevance in nature and industry, the near-zero growth physiology of S. cerevisiae under conditions where growth is limited by the supply of non-energy...

Molecular responses of Saccharomyces cerevisiae to near-zero growth rates

Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: Transcriptome analysis of anaerobic retentostat cultures

Extremely low specific growth rates (below 0.01 h?1) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is...

Physiology and robustness of Saccharomyces cerevisiae at near-zero specific growth rates