How to evaluate a monitoring system for adaptive policies: criteria for signposts selection and their model-based evaluation

Adaptive policies have emerged as a valuable strategy for dealing with uncertainties by recognising the capacity of systems to adapt over time to new circumstances and surprises. The efficacy of adaptive policies hinges on detecting on-going change and ensuring that actions are indeed taken if and when necessary. This is operationalised by...

Stoichiometry and kinetics of single and mixed substrate uptake in Aspergillus niger

In its natural environment, the filamentous fungus Aspergillus niger grows on decaying fruits and plant material, thereby enzymatically degrading the lignocellulosic constituents (lignin, cellulose, hemicellulose, and pectin) into a mixture of mono- and oligosaccharides. To investigate the kinetics and stoichiometry of growth of this fungus...

Integrated isotope-assisted metabolomics and ¹³C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger

Background: Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. Results: In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-producing strain A. niger...

Sequence-based learning algorithms for understanding and improving protein characteristics

The development of high-throughput measurement techniques resulted in rapidlyincreasing amounts of biological data, which made computational methodsessential for biological research. Hence, the field of bioinformatics emergedthat since plays an important role in storing, making accessible, integrating,and analysing different types of biological...

Development of tools for quantitative intracellular metabolomics of Aspergillus niger chemostat cultures

In view of the high citric acid production capacity of Aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. Quantitative metabolomics is an important omics tool in a synthetic biology approach to develop A. niger for the production of these acids. Such...

Fine Sediment Infill Reduction in Offshore Dredged Approach Channel

An offshore terminal is proposed to be constructed adjacent to the entrance of an Estuary in the Niger Delta. A dredged approach channel of around 10 km long, 250 m wide and to a depth of -14 m LAT is required to allow ships to reach the terminal. From earlier research it is found that high transport rates of fine sediment, in the order of 10...

The transcriptomic fingerprint of glucoamylase over-expression in Aspergillus niger

Background: Filamentous fungi such as Aspergillus niger are well known for their exceptionally high capacity for secretion of proteins, organic acids, and secondary metabolites and they are therefore used in biotechnology as versatile microbial production platforms. However, system-wide insights into their metabolic and secretory capacities are...

Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress

Background: HacA/Xbp1 is a conserved bZIP transcription factor in eukaryotic cells which regulates gene expression in response to various forms of secretion stress and as part of secretory cell differentiation. In the present study, we replaced the endogenous hacA gene of an Aspergillus niger strain with a gene encoding a constitutively active...

Integrated water resources modelling of the Upper Niger River (Mali)

Meer dan zandkastelen: Architektonisch reisverhaal

This book is the account of a journey through the Sahara, from the Maghreb to the countries of the Sahel. The writer describes the history of the area and the technology, styles and backgrounds of its architecture. This is alternated with an account of the writer's experiences during the trip through a port of the continent whose roads are...

Overlaatconstructie te Onitsha, Nigeria

In de rivier de Niger in Nigeria zal ter hoogte van Onitsha een dam worden gebouwd. Deze dam met een lengte van ongeveer 3200 m moet een reservoir creeren door opstuwing van het water van de Niger. De door de gehele dam veroorzaakte waterstandsverhoging beoogt in de eerste plaats opwekking van energie middels een waterkrachtcentrale. Doordat het...

Schutsluis bij Onitsha (Nigeria)

In de toekomstige dam, die gepland is in de buurt van Onitsha in Nigeria, moet een mogelijkheid worden gecreëerd om de scheepvaart doorgang hoofdzakelijk te kunnen verschaffen. In de toekomst zal er gevaren worden met vierbaksduweenheden, dus de schutmogelijkheid moet deze combinatie kunnen doorschutten. In eerste instantie is er gedacht om de...

River studies and recommendations on improvement of Niger and Benué

Extensive study or the rivers Niger and Benué in order to describe the hydraulics and morphology with the aim to improve inland navigation in Nigeria. Part I gives a summary of the original NEDECO studies and their results as contained in the Report, without summarising the information obtained from other sources. This is followed by a...