Searched for: subject%3A%22Protein%22
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Tišma, M. (author)
This thesis explores the mechanisms that underlie chromosome organization in bacteria. Bacteria are considered amongst the simplest living organisms on our planet. They lack the cellular organization found in other domains of life (Archaea or Eukaryotics) and often have simpler life cycles. Over the past decade, we gained increasing knowledge...
doctoral thesis 2024
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Imani, Amin (author), Rahimi, E. (author), Lekka, Maria (author), Andreatta, Francesco (author), Magnan, Michele (author), Gonzalez Garcia, Y. (author), Mol, J.M.C. (author), Raman, R. K. Singh (author), Fedrizzi, Lorenzo (author), Asselin, Edouard (author)
Mg and its alloys are promising biodegradable materials for orthopedic implants and cardiovascular stents. The first interactions of protein molecules with Mg alloy surfaces have a substantial impact on their biocompatibility and biodegradation. We investigate the early-stage electrochemical, chemical, morphological, and electrical surface...
journal article 2024
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Chen, L.M. (author), Beck, Paula (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)
Abstract: Anionic polymers, such as heparin, have been widely applied in the chemical and medical fields, particularly for binding proteins (e.g., fibroblast growth factor 2 (FGF-2) and histones). However, the current animal-based production of heparin brings great risks, including resource shortages and product contamination. Recently,...
journal article 2024
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Yin, Xinjian (author), Gong, Wenzhong (author), Zeng, Yujing (author), Qiu, Hulin (author), Liu, Lan (author), Hollmann, F. (author), Chen, Bishuang (author)
The biocatalytic oxidative deamination of β-amino alcohols holds significant practical potential in kinetic resolution and/or deracemization process to access (R)-β-amino alcohols. This study exemplifies a notable instance of acquisition and utilization of this valuable oxidative deamination activity. Initially, the mutation N261M (M0) was...
journal article 2024
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Pankratov, Dmitrii (author), Hidalgo Martinez, Silvia (author), Karman, Cheryl (author), Gerzhik, Anastasia (author), Gomila, Gabriel (author), Trashin, Stanislav (author), Boschker, H.T.S. (author), Geelhoed, Jeanine S. (author), Meysman, F.J.R. (author)
Cable bacteria are filamentous, multicellular microorganisms that display an exceptional form of biological electron transport across centimeter-scale distances. Currents are guided through a network of nickel-containing protein fibers within the cell envelope. Still, the mechanism of long-range conduction remains unresolved. Here, we...
journal article 2024
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Neijenhuis, T. (author), Le Bussy, Olivier (author), Geldhof, Geoffroy (author), Klijn, M.E. (author), Ottens, M. (author)
Protein-based biopharmaceuticals require high purity before final formulation to ensure product safety, making process development time consuming. Implementation of computational approaches at the initial stages of process development offers a significant reduction in development efforts. By preselecting process conditions, experimental...
journal article 2024
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Ingram, A.A. (author), Oike, K. (author)
Astonishing progress has been achieved in unlocking new-to-nature biocatalysis in the past decades. The progress in protein engineering enabled research to efficiently incorporate artificial structural elements into enzyme design. Recent trends include cofactor mimetics, artificial metalloenzymes and non-canonical amino acids. In this...
journal article 2024
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Overtoom-van Aartrijk, C.M.M. (author)
The protease FtsH is a membrane anchored protease that degrades misfolded (membrane) proteins. To study the mechanism behind unfolding and translocation with optical tweezers, the protease had to be purified. However, full-length FtsH has hardly been studied as solubilizing this membrane anchored protease is challenging. Our group (Dr. V....
doctoral thesis 2023
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Hoebus, Laura (author)
In cold regions, the formation and accumulation of ice can cause safety hazards and impede proper operation of equipment. By example, ice accumulation on aircraft wings can increase drag, increase weight, reduce upward force and decrease aircraft speed. <br/><br/>For these reasons, proper anti- or de-icing techniques need to be developed. These...
master thesis 2023
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Dieben, Daniek (author)
Our current food system puts a lot of pressure on our planet, global health, animal welfare, and creates an unfair agricultural economy. One of the strategies to decrease this pressure while serving the growing population is the protein transition. As Weinricht (2018) states, “Shifting our collective diet from eating mainly animal proteins to...
master thesis 2023
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Dijkstra, Maaike (author)
The Protein Transition is a societal transition that resolves around shifting society towards consuming more plant-based proteins instead of animal proteins. There are numerous drivers for this transition, the most well-known and considerable ones being climate change, animal welfare, public health and unfair agricultural economics.<br/><br/>To...
master thesis 2023
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Hebbar, Akshatha (author), Dey, P. (author), Vatti, Anoop Kishore (author)
The ability of neat deep eutectic solvents (DESs) to influence protein structure and function has gained considerable interest due to the unstable nature of enzymes or therapeutic proteins, which are often exposed to thermal, chemical, or mechanical stresses when handled at an industrial scale. In this study, we simulated a model globular...
journal article 2023
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Xu, J. (author), Fahmy-Garcia, Shorouk (author), Wesdorp, Marinus A. (author), Forte, Lucia (author), De Luca, Claudio (author), Filardo, Giuseppe (author), Labberté, Margot (author), Kok, Joeri (author), Nickel, Joachim (author), van Osch, G.J.V.M. (author)
Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet...
journal article 2023
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Safdar, Luqman B. (author), Foulkes, M. John (author), Dr. Kleiner, F.K. (author), Searle, Iain R. (author), Bhosale, Rahul A. (author), Fisk, Ian D. (author), Boden, Scott A. (author)
Rising demands for protein worldwide are likely to drive increases in livestock production, as meat provides ∼40% of dietary protein. This will come at a significant environmental cost, and a shift toward plant-based protein sources would therefore provide major benefits. While legumes provide substantial amounts of plant-based protein,...
review 2023
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Deng, Z. (author), Ferreira, Ana Lucia Morgado (author), Spanjers, H. (author), van Lier, J.B. (author)
Bovine serum albumin (BSA) and casein (CAS) were used in batch tests to compare the protein degradation in the presence and absence of carbohydrates and volatile fatty acids (VFAs). The modified Gompertz model was applied to estimate reaction rates. The results showed that deamination was the rate-limiting step, with a rate ranging between 2...
journal article 2023
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Furukita Baptista, I.B. (author), Schifferstein, Hendrik N.J. (author)
The market growth of plant-based alternatives to animal food products pushes agencies around the world to discuss specific regulations regarding their communication, terminology, and packaging design. We created and tested 18 packages of plant-based milk and plant-based chicken meat varying the “animalness” of terminology, container, image,...
journal article 2023
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Neves Sao Pedro, M. (author), Eppink, Michel H.M. (author), Ottens, M. (author)
The lack of process analytical technologies able to provide real-time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is a major critical quality attribute (CQA) with several known process...
journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)
Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...
journal article 2023
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Gomez de Santos, Patricia (author), González-Benjumea, Alejandro (author), Fernandez-Garcia, Angela (author), Aranda, Carmen (author), Wu, Y. (author), But, A. (author), Molina-Espeja, Patricia (author), Zhang, W. (author), Hollmann, F. (author)
The hydroxylation of fatty acids is an appealing reaction in synthetic chemistry, although the lack of selective catalysts hampers its industrial implementation. In this study, we have engineered a highly regioselective fungal peroxygenase for the ω-1 hydroxylation of fatty acids with quenched stepwise over-oxidation. One single mutation near...
journal article 2023
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Meng, X. (author), Ganapathy, S. (author), van Roemburg, Lars (author), Post, M.G. (author), Brinks, D. (author)
Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a...
review 2023
Searched for: subject%3A%22Protein%22
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