Searched for: subject%3A%22Single%255C-molecule%22
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Filius, M. (author), van Wee, R.G. (author), Joo, C. (author)
Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short...
book chapter 2023
document
Kalafut, B.S. (author), Skinner, G.M. (author), Visscher, K. (author)
To access the genetic code to be transcribed to RNA, RNA polymerases must first open a “transcription bubble” in the DNA. Structural studies suggest that the minimal model of initiation by T7 bacterophage RNA polymerase (T7 RNAP) consists of two distinct steps: initial binding, in which the T7 RNAP binds to and bends the DNA, and opening,...
conference paper 2009
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Dietrich, H.R.C. (author), Young, I.T. (author), Garini, Y. (author)
The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact...
conference paper 2006
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Stallinga, S. (author)
Localization microscopy for imaging at the nano-scale relies on the quality of fitting the emitter positions from the measured light spots. The type and magnitude of the noise in the detection process, the background light level and the Point Spread Function model that is used in the fit are of paramount importance for the precision and accuracy...
conference paper 2015
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Parikesit, G.O.F. (author), Kutchoukov, V.G. (author), Bossche, A. (author), Young, I.T. (author), Garini, Y. (author)
We report on the progress of a novel nanofluidic device for detecting and manipulating single molecules in solution. This paper discusses the development of an earlier proposed molecule separation method, where electrokinetic forces separate different molecules based on their masses and charges. Optical imaging using confocal microscopy is...
conference paper 2005
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Dietrich, H.R.C. (author), Vermolen, B.J. (author), Rieger, B. (author), Young, I.T. (author), Garini, Y. (author)
Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been...
conference paper 2007
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Dietrich, H.R.C. (author), Young, I.T. (author), Garini, Y. (author)
The intense research in proteomics is demanding for fast, reliable and easy-to-use methods in order to study the proteome. In this proceeding we report the development of such a novel research tool based on spectral imaging and Resonance Light Scattering gold particles. This method will allow the study of DNA-protein interactions. We suggest a...
conference paper 2005
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Parikesit, G.O.F. (author), Kutchoukov, V.G. (author), Van Oel, W. (author), Lung, G.L. (author), Bossche, A. (author), Young, I.T. (author), Garini, Y. (author)
We present the latest progress on a novel technology for detecting and manipulating solution of single molecules in nanofluidic channels. This paper explains the design and fabrication of nanofluidic chip and its interface, molecule manipulation technique being used, and the optical detection method employed. Single molecule detections are...
conference paper 2004
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Martin, C.A. (author)
During the past decades the downscaling of integrated circuits has been governed by Moore's law, which predicts device dimensions on the order of 10 nm in 2020. Fundamental research in molecular electronics explores the possibility of fabricating such nanoscale devices from single molecules, which offer the prospects of chemical tunability and...
doctoral thesis 2010
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Vlijm, R. (author)
The main focus of this thesis is a better understanding of the basic compaction mechanism of our DNA using multiple single-molecule techniques. The stretched-out length of our DNA is enormous compared with the dimensions of a cell. To make DNA fit within a cell it is systematically wrapped around proteins (histone octamers), forming nucleosomes....
doctoral thesis 2014
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Chandradoss, S.D. (author)
doctoral thesis 2016
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Osorio Oliveros, E.A. (author)
This thesis describes three-terminal transport measurements through single molecules. The interest in this field stems from the dream that single molecules will form the building blocks for future nanoscale electronic devices. The advantages are their small size -nanometers-, and their synthetic tailorability which allows the molecules to be...
doctoral thesis 2009
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van der Heijden, A.H. (author)
What happens when your DNA breaks? This thesis describes experimental work on the single-molecule level focusing on the interaction between DNA and DNA-repair proteins, in particular bacterial RecA and human Rad51, involved in homologous recombination. Homologous recombination and its central event of DNA strand exchange are essential processes...
doctoral thesis 2007
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Zyazin, A.S. (author)
doctoral thesis 2012
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Van Oene, M.M. (author)
This thesis describes developments in the characterization of torque-spectroscopy techniques, in particular magnetic and optical tweezers, with the goal of employing these techniques in studies on the bacterial flagellar motor of Escherichia coli.
doctoral thesis 2016
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Bezrukavnikov, S.V. (author)
Since the first protein structure reported, the understanding of how proteins fold so fast and efficiently into such peculiar shapes has quickly become one of the major problems of biology. Protein folding in the living cell features a new level of complexity due to highly crowded cellular environment. In order to overcome this problem, cells...
doctoral thesis 2015
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Seldenthuis, J.S. (author)
In single-molecule junctions, the behavior of a device is determined by the properties of an individual molecule. In this thesis we develop several models to describe both electrical and mechanical effects in such devices, which can be used to design molecules with a specific functionality. We show how the resistance of a molecule varies with...
doctoral thesis 2011
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Perrin, M.L. (author)
In the pursuit of down-sizing electronic components, the ultimate limit is the use of single molecules as functional devices. Molecules can also provide additional functionalities compared to semiconductors. Using synthetic chemistry, an almost endless choice of molecular structures and compositions is available. With the proper knowledge, one...
doctoral thesis 2015
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Huang, Z. (author)
Heterogeneity is a general feature in biological system. In order to avoid possible misleading effects of ensemble averaging, and to ensure a correct understanding of the biological system, it is very important to look into individuals, such as a single bio-molecule or a single cell, for details. The size of a single bio-molecule/cell typically...
doctoral thesis 2012
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Van Loenhout, M.T.J. (author)
This thesis describes a series of single-molecule experiments aimed at understanding the physical properties of DNA itself and the proteins that interact with it. We developed and applied sensitive techniques that allowed us to directly probe the conformation and interactions of individual DNA molecules and proteins. Magnetic and optical...
doctoral thesis 2012
Searched for: subject%3A%22Single%255C-molecule%22
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