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Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Hung, S. (author), Llobet Rosell, Arnau (author), Jurriens, Daphne (author), Soloviev, O.A. (author), Kapitein, Lukas C. (author), Grußmayer, K.S. (author), Neukomm, Lukas J. (author), Verhaegen, M.H.G. (author), Smith, C.S. (author)

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high...

journal article 2022

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Challenges in simulating advanced control methods for AO

Piscaer, P.J. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author)

This paper discusses various practical problems arising in the design and simulation of predictive control methods for adaptive optics. Although there has been increased attention towards optimal prediction and control methods for AO systems, they are often tested in simplified simulation environments. The use of advanced AO simulators...

conference paper 2020

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Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

Quintavalla, M. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author), Bijlsma, H.K. (author), Verstraete, H.R.G.W. (author), Bonora, S. (author)

Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL)...

conference paper 2018

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Practical guidelines for implementing adaptive optics in fluorescence microscopy

Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)

In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...

conference paper 2018

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Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy

Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)

In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...

conference paper 2018

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Hybrid adaptive and computational light-sheet fluorescence microscopy

Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...

conference paper 2018

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Wavefront control with a multi-actuator adaptive Lens in imaging applications

Mocci, J. (author), Cua, M. (author), Lee, S. (author), Jian, Y (author), Pozzi, P. (author), Quintavalla, M. (author), Trestino, C. (author), Verstraete, H.R.G.W. (author), Wahl, D. (author), Muradore, R. (author), Zawadzki, R.J. (author), Verhaegen, M.H.G. (author), Sarunic, M.V. (author), Bonora, S. (author)

The use of adaptive lenses instead of deformable mirrors can simplify the implementation of an adaptive optics system. The recently introduced Multi-actuator Adaptive Lens (MAL) can be used in closed loop with a wavefront sensor to correct for time-variant wavefront aberrations. The MAL can guarantee a level of correction and a response time...

conference paper 2017

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Phase retrieval from multiple binary masks generated speckle patterns

Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)

We present a reference-less and time-multiplexing phase retrieval method by making use of the digital micromirror device (DMD). In this method, the DMD functions not only as a flexible binary mask which modulates the optical field, but also as a sampling mask for measuring corresponding phases, which makes the whole setup simple and robust. The...

conference paper 2016

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Adaptive optics for confocal laser scanning microscopy with adjustable pinhole

Yoo, H.W. (author), van Royen, M.E. (author), van Cappellen, WA (author), Houtsmuller, AB (author), Verhaegen, M.H.G. (author), Schitter, G (author)

The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with...

conference paper 2016

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Feedforward operation of a lens setup for large defocus and astigmatism correction

Verstraete, H.R.G.W. (author), Almasian, M. (author), Pozzi, P. (author), Bilderbeek, R (author), Kalkman, J. (author), Faber, DJ (author), Verhaegen, M.H.G. (author)

In this manuscript, we present a lens setup for large defocus and astigmatism correction. A deformable defocus lens and two rotational cylindrical lenses are used to control the defocus and astigmatism. The setup is calibrated using a simple model that allows the calculation of the lens inputs so that a desired defocus and astigmatism are...

conference paper 2016

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Light-sheet optimization for microscopy

Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)

Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...

conference paper 2016

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Sensorless adaptive optics system based on image second moment measurements

Agbana, T.E. (author), Yang, H. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)

This paper presents experimental results of a static aberration control algorithm based on the linear relation be- tween mean square of the aberration gradient and the second moment of point spread function for the generation of control signal input for a deformable mirror (DM). Results presented in the work of Yang et al.1 suggested a good...

conference paper 2016

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Wavefront coding with adaptive optics

Agbana, T.E. (author), Soloviev, O. (author), Bezzubik, V. (author), Patlan, V. (author), Verhaegen, M. (author), Vdovin, G.V. (author)

We have implemented an extended depth of field optical system by wavefront coding with a micromachined membrane deformable mirror. This approach provides a versatile extension to standard wavefront coding based on fixed phase mask. First experimental results validate the feasibility of the use of adaptive optics for variable depth wavefront...

conference paper 2015

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Curvature sensing with a Shack-Hartmann sensor

Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)

Shack-Hartmann (SH) sensor, based on sampling of wavefront tilts in subapertures, is a simple, reliable, and widely used in adaptive optics wavefront sensor. A wavefront curvature sensor has the advantage of providing the results suitable for direct control of membrane and bimorph deformable mirrors [1], but requires linear registration of...

conference paper 2015

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Lensless coherent imaging by sampling of the optical field with digital micromirror device

Vdovin, Gleb (author), Gong, H. (author), Soloviev, O.A. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author)

We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude in the pupil of the imaging system, without any reference beam,...

journal article 2015

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Fast & Furious focal-plane wavefront sensing

Korkiakoski, V.A. (author), Keller, C.U. (author), Doelman, N. (author), Kenworthy, M. (author), Otten, G. (author), Verhaegen, M.H.G. (author)

We present two complementary algorithms suitable for using focal-plane measurements to control a wavefront corrector with an extremely high-spatial resolution. The algorithms use linear approximations to iteratively minimize the aberrations seen by the focal-plane camera. The first algorithm, Fast & Furious (FF), uses a weak-aberration...

journal article 2014

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Optimization-based wavefront sensorless adaptive optics for multiphoton microscopy

Antonello, J. (author), Van Werkhoven, T. (author), Verhaegen, M. (author), Truong, H.H. (author), Keller, C.U. (author), Gerritsen, H.C. (author)

Optical aberrations have detrimental effects in multiphoton microscopy. These effects can be curtailed by implementing model-based wavefront sensorless adaptive optics, which only requires the addition of a wavefront shaping device, such as a deformable mirror (DM) to an existing microscope. The aberration correction is achieved by maximizing a...

journal article 2014

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Snapshot coherence-gated direct wavefront sensing for multi-photon microscopy

Van Werkhoven, T.I.M. (author), Antonello, J. (author), Truong, H.H. (author), Verhaegen, M. (author), Gerritsen, H.C. (author), Keller, C.U. (author)

Deep imaging in turbid media such as biological tissue is challenging due to scattering and optical aberrations. Adaptive optics has the potential to compensate the tissue aberrations. We present a wavefront sensing scheme for multi-photon scanning microscopes using the pulsed, near-infrared light reflected back from the sample utilising...

journal article 2014

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Receding-horizon adaptive contyrol of aero-optical wavefronts

Tesch, J. (author), Gibson, S. (author), Verhaegen, M. (author)

A new method for adaptive prediction and correction of wavefront errors in adaptive optics (AO) is introduced. The new method is based on receding-horizon control design and an adaptive lattice filter. Experimental results presented illustrate the capability of the new adaptive controller to predict and correct aero-optical wavefronts derived...

journal article 2013

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Joint optimization of phase diversity and adaptive optics: Demonstration of potential

Korkiakoski, V. (author), Keller, C.U. (author), Doelman, N. (author), Fraanje, P.R. (author), Verhaegen, M.H.G. (author)

We study different possibilities to use adaptive optics (AO) and phase diversity (PD) together in a jointly optimized system. The potential of the joint system is demonstrated through numerical simulations. We find that the most significant benefits are obtained from the improved deconvolution of AO-corrected wavefronts and the additional...

journal article 2011