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Luo, Zhiyuan (author), Qiao, Li (author), Chen, Haomin (author), Mao, Zhili (author), Wu, Shujiao (author), Ma, Bianqin (author), Xie, Tian (author), Wang, Anming (author), Pei, Xiaolin (author), Sheldon, R.A. (author)The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...journal article 2024
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Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine DehydrogenaseJongkind, E.P.J. (author), Fossey-Jouenne, Aurélie (author), Mayol, Ombeline (author), Zaparucha, Anne (author), Vergne-Vaxelaire, Carine (author), Paul, C.E. (author)Access to chiral amines with more than one stereocentre remains challenging, although an increasing number of methods are emerging. Here we developed a proof-of-concept bi-enzymatic cascade, consisting of an ene reductase and amine dehydrogenase (AmDH), to afford chiral diastereomerically enriched amines in one pot. The asymmetric reduction...journal article 2021
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Chen, B. (author), Medici, R. (author), van der Helm, M. (author), van Zwet, Y.A.A. (author), Gjonaj, L. (author), van der Geest, R.C.S. (author), Otten, L.G. (author), Hanefeld, U. (author)Rhodococcus strains are ubiquitous in nature and known to metabolise a wide variety of compounds. At the same time, asymmetric reduction of C=C bonds is important in the production of high-valued chiral building blocks. In order to evaluate if Rhodococci can be used for this task, we have probed several Rhodococcus rhodochrous and R....journal article 2018
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Cofactor-Free, Direct Photoactivation of Enoate Reductases for the Asymmetric Reduction of C=C BondsLee, Sahng Ha (author), Choi, Da Som (author), Pesic, M. (author), Lee, Yang Woo (author), Paul, C.E. (author), Hollmann, F. (author), Park, Chan Beum (author)Enoate reductases from the family of old yellow enzymes (OYEs) can catalyze stereoselective trans-hydrogenation of activated C=C bonds. Their application is limited by the necessity for a continuous supply of redox equivalents such as nicotinamide cofactors [NAD(P)H]. Visible light-driven activation of OYEs through NAD(P)H-free, direct...journal article 2017