Exploring Giant Unilamellar Vesicle Production for Artificial Cells – Current Challenges and Future Directions

Creating an artificial cell from the bottom up is a long-standing challenge and, while significant progress has been made, the full realization of this goal remains elusive. Arguably, one of the biggest hurdles that researchers are facing now is the assembly of different modules of cell function inside a single container. Giant unilamellar...

Synthetic Cell Aspirations: A Toolbox for Building a Membrane Container from the Bottom Up

Everything that we consider alive, be it plants, dogs, bacteria or humans, is composed of the same microscopic building blocks: cells. While cells between and even within these organisms can look and behave very differently, they all share the same key functionalities: they can grow, they can divide to proliferate, they can eat and metabolise to...

DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles

Giant unilamellar vesicles (GUVs) are cell-sized aqueous compartments enclosed by a phospholipid bilayer. Due to their cell-mimicking properties, GUVs have become a widespread experimental tool in synthetic biology to study membrane properties and cellular processes. In stark contrast to the experimental progress, quantitative analysis of GUV...

Actomyosin-Driven Division of a Synthetic Cell

One of the major challenges of bottom-up synthetic biology is rebuilding a minimal cell division machinery. From a reconstitution perspective, the animal cell division apparatus is mechanically the simplest and therefore attractive to rebuild. An actin-based ring produces contractile force to constrict the membrane. By contrast, microbes and...