CENP-B-mediated DNA loops regulate activity and stability of human centromeres

Chromosome inheritance depends on centromeres, epigenetically specified regions of chromosomes. While conventional human centromeres are known to be built of long tandem DNA repeats, much of their architecture remains unknown. Using single-molecule techniques such as AFM, nanopores, and optical tweezers, we find that human centromeric DNA...

CENP-A and H3 Nucleosomes Display a Similar Stability to Force-Mediated Disassembly

Centromere-specific nucleosomes are a central feature of the kinetochore complex during mitosis, in which microtubules exert pulling and pushing forces upon the centromere. CENP-A nucleosomes have been assumed to be structurally unique, thereby providing resilience under tension relative to their H3 canonical counterparts. Here, we directly test...

Nuclear Architecture: Image Processing and Analyses

Cancer is one of the most well-known groups of diseases that finds its cause in cells having chromosomal aberrations. How and why these aberrations can occur is one of the most important questions asked in modern molecular biology. In the last decades it has become clear that gene regulation in the nucleus, where the chromosomes reside, is...