Correlative light and electron microscopy reveals fork-shaped structures at actin entry sites of focal adhesions

Focal adhesions (FAs) are the main cellular structures to link the intracellular cytoskeleton to the extracellular matrix. FAs mediate cell adhesion, are important for cell migration and are involved in many (patho)-physiological processes. Here we examined FAs and their associated actin fibres using correlative fluorescence and scanning...

The 2018 correlative microscopy techniques roadmap

Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x...

Correlating 3D light to 3D electron microscopy for systems biology

Whilst a ‘resolution revolution’ has taken place at the macromolecular scale in both electron microscopy and light microscopy, a ‘volume revolution’ has taken place at the tissue and organism level in both imaging modalities. At both ends of the scale – resolution and volume – there are concerted efforts to link the information from light and...