Searched for: subject%3A%22correlative%255C+light%255C+and%255C+electron%255C+microscopy%22
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Lane, R. (author)
Multi-modal imaging techniques have become essential for better understanding fundamental questions in cell biology such as disease progression. While individual microscopy methods have rapidly advanced in recent years, the information content of any one imaging technique is limited to the type of contrast that particular technique is sensitive...
doctoral thesis 2022
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Lane, R. (author), Wolters, Anouk H.G. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of acquisition and the difficulty to provide targeted biological information. We developed a 3D...
journal article 2022
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Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...
journal article 2021
document
Srinivasa Raja, A. (author), de Boer, Pascal (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Electron microscopy is crucial for imaging biological ultrastructure at nanometer resolution. However, electron irradiation also causes specimen damage, reflected in structural and chemical changes that can give rise to alternative signals. Here, luminescence induced by electron-beam irradiation is reported across a range of materials widely...
journal article 2021
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Vos, Y. (author), Lane, R. (author), Peddie, Chris J. (author), Wolters, A.E. (author), Hoogenboom, J.P. (author)
The authors present the application of a retarding field between the electron objective lens and sample in an integrated fluorescence and electron microscope. The retarding field enhances signal collection and signal strength in the electron microscope. This is beneficial for samples prepared for integrated fluorescence and electron...
journal article 2020
Searched for: subject%3A%22correlative%255C+light%255C+and%255C+electron%255C+microscopy%22
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