Searched for: subject%3A%22ene%255C-reductase%22
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document
Heckmann, C.M. (author), Bürgler, Moritz (author), Paul, C.E. (author)
The unmatched chemo-, regio-, and stereoselectivity of enzymes renders them powerful catalysts in the synthesis of chiral active pharmaceutical ingredients (APIs). Inspired by the discovery route toward the LPA<sub>1</sub>-antagonist BMS-986278, access to the API building block (1S,3R)-3-hydroxycyclohexanecarbonitrile was envisaged using an...
journal article 2024
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Joseph Srinivasan, Shiny (author), Cleary, Sarah E. (author), Ramirez, Miguel A. (author), Reeve, Holly A. (author), Paul, C.E. (author), Vincent, Kylie A. (author)
A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H<sub>2</sub> as a simple, completely atom-economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25–70 °C)...
journal article 2021
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Yoon, Jaeho (author), Lee, S.H. (author), Tieves, F. (author), Rauch, M.C.R. (author), Hollmann, F. (author), Park, Chan Beum (author)
We report visible light-driven, asymmetric hydrogenation of C=C bonds using an ene-reductase from Thermus scotoductus SA-01 (TsOYE) and a light-harvesting dye (rose bengal, RB) co-immobilized in an alginate hydrogel. Highly efficient encapsulation of RB in alginate hydrogel was achieved using the intrinsic affinity between TsOYE and RB,...
journal article 2019
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Scholtissek, Anika (author), Gädke, Eric (author), Paul, C.E. (author), Westphal, Adrie H. (author), Van Berkel, Willem J.H. (author), Tischler, Dirk (author)
Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from Rhodococcus opacus 1CP (WT) as well as its engineered variants C25A, C25S and C25G. OYERo2a in its redox resting state ...
journal article 2018
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Chen, B. (author), Medici, R. (author), van der Helm, M. (author), van Zwet, Y.A.A. (author), Gjonaj, L. (author), van der Geest, R.C.S. (author), Otten, L.G. (author), Hanefeld, U. (author)
Rhodococcus strains are ubiquitous in nature and known to metabolise a wide variety of compounds. At the same time, asymmetric reduction of C=C bonds is important in the production of high-valued chiral building blocks. In order to evaluate if Rhodococci can be used for this task, we have probed several Rhodococcus rhodochrous and R....
journal article 2018
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Chen, B. (author)
?-Hydroxy carbonyl compounds are an important class of compounds often found as a common structural motif in natural products. Although the molecules themselves look rather simple, their synthesis can be challenging. Water addition to conjugated C = C bonds opens up a straightforward route for the preparation of ?-hydroxy carbonyl compounds....
doctoral thesis 2015
document
Gargiulo, S. (author)
Oxidation of alcohols is a reaction of major interest for organic chemistry. However, the most common chemical routes developed so far involve the use of toxic or hazardous reagents or catalysts that often lack good chemoselectivity. In this respect, alcohol dehydrogenases (ADHs) represent a very valuable biocatalytic alternative, as they can...
doctoral thesis 2015
Searched for: subject%3A%22ene%255C-reductase%22
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