Searched for: subject:"evolutionary%5C+engineering"
(1 - 7 of 7)
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Verhoeven, M.D. (author), de Valk, S.C. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
D-Glucose, D-xylose and L-arabinose are major sugars in lignocellulosic hydrolysates. This study explores fermentation of glucose-xylose-arabinose mixtures by a consortium of three ‘specialist’ Saccharomyces cerevisiae strains. A D-glucose- and L-arabinose-tolerant xylose specialist was constructed by eliminating hexose phosphorylation in an...
journal article 2018
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Brickwedde, A. (author), van den Broek, M.A. (author), Geertman, Jan Maarten A. (author), Magalhães, Frederico (author), Kuijpers, Niels G.A. (author), Gibson, Brian (author), Pronk, J.T. (author), Daran, J.G. (author)
The lager brewing yeast Saccharomyces pastorianus, an interspecies hybrid of S. eubayanus and S. cerevisiae, ferments maltotriose, maltose, sucrose, glucose and fructose in wort to ethanol and carbon dioxide. Complete and timely conversion ("attenuation") of maltotriose by industrial S. pastorianus strains is a key requirement for process...
journal article 2017
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Gonzalez Ramos, D. (author), Gorter de Vries, A.R. (author), Grijseels, Sietske S. (author), Swinnen, Steve (author), van den Broek, M.A. (author), Nevoigt, Elke (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
Background: Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before...
journal article 2016
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Kozak, B.U. (author), van Rossum, Harmen M. (author), Niemeijer, M.S. (author), van Dijk, M. (author), Benjamin, Kirsten (author), Wu, Liang (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
In Saccharomyces cerevisiae ethanol dissimilation is initiated by its oxidation and activation to cytosolic acetyl-CoA. The associated consumption of ATP strongly limits yields of biomass and acetyl-CoA-derived products. Here, we explore the implementation of an ATP-independent pathway for acetyl-CoA synthesis from ethanol that, in theory,...
journal article 2016
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Oud, B. (author)
Reverse engineering is the study of discovering the structure, function and operation of a device or system with the express aim to reconstruct its key functionalities. This principle is applied to many disciplines, from military, through computer engineering, to health, but also in metabolic engineering. In this context, reverse metabolic...
doctoral thesis 2013
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Gonzalez-Ramos, D. (author), Van den Broek, M. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M.G. (author)
Background n-Butanol and isobutanol produced from biomass-derived sugars are promising renewable transport fuels and solvents. Saccharomyces cerevisiae has been engineered for butanol production, but its high butanol sensitivity poses an upper limit to product titers that can be reached by further pathway engineering. A better understanding of...
journal article 2013
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Kuijper, S.M. (author)
For various reasons mankind is looking for alternatives for fossil fuels. One of these alternatives is ethanol made from plant biomass. However, the plant material when broken down by hydrolysis into its sugar monomers contains a significant amount of xylose, a 5-carbon-sugar or pentose. Contrary to the sugars with 6 carbon atoms (hexoses) wild...
doctoral thesis 2006
Searched for: subject:"evolutionary%5C+engineering"
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