- document
-
Tišma, M. (author)This thesis explores the mechanisms that underlie chromosome organization in bacteria. Bacteria are considered amongst the simplest living organisms on our planet. They lack the cellular organization found in other domains of life (Archaea or Eukaryotics) and often have simpler life cycles. Over the past decade, we gained increasing knowledge...doctoral thesis 2024
- document
-
Yang, X. (author), Shin, D. (author), Yu, Z. (author), Watanabe, Kenji (author), Taniguchi, Takashi (author), Babenko, Vitaliy (author), Hofmann, Stephan (author), Caneva, S. (author)Fluorescence imaging is an invaluable tool to investigate biomolecular dynamics, mechanics, and interactions in aqueous environments. Two-dimensional materials offer large-area, atomically smooth surfaces for wide-field biomolecule imaging. Despite the success of graphene for on-chip biosensing and biomolecule manipulation, its strong...journal article 2024
- document
-
Kaaks, B.J. (author), Couweleers, S.D. (author), Lathouwers, D. (author), Kloosterman, J.L. (author), Rohde, M. (author)This work presents two color LIF temperature measurements for the transient freezing in a square channel under laminar flow conditions. This is the first time non-intrusive temperature measurements were performed within the thermal boundary layer during the transient growth of an ice layer in internal flow. A combination of a local outlier...journal article 2024
- document
-
Kesapidou, Evi (author)Nanopores are narrow channels in cell membranes that control the passage of small molecules. In recent years, they have been repurposed for diverse applications, including single molecule sensing and drug delivery. Due to the small diameters of conventional protein-based, current research efforts are directed to designing nanopores from other...master thesis 2023
- document
-
Gomez Jaramillo, Laura Sofia (author)Recycled concrete aggregates (RCA) have been recognized as a sustainable solution to satisfy the demand and address the environmental impacts of the concrete industry. However, construction engineers and contractors still have reservations about RCA due to their heterogeneous origin and lower quality compared to natural aggregates. It is...master thesis 2023
- document
-
Klughammer, N. (author)In this thesis, we have introduced palladium zero-mode waveguides (Pd ZMWs) as a valuable tool for biophysics research and have showcased several applications thereof. We started by using them as single-molecule sensors for free translocations of fluorophores, proteins, and voltage-driven translocations of DNA. By studying salt-gradient driven...doctoral thesis 2023
- document
-
Neves Sao Pedro, M. (author)Continuous biomanufacturing is considered the future phase for the optimization of production processes in the biopharmaceutical industry. Productivity, product quality and consistency are greatly improved while production costs and environmental footprint are drastically reduced. The manufacturing of monoclonal antibodies (mAbs), an important...doctoral thesis 2023
- document
-
Krishnan, Aditya (author)The growing importance of rare earth elements (REEs) in sustainable technologies necessitates anefficient assessment of potential resource targets within the European Union. Traditional analyticaltechniques for REE determination have drawbacks like destructive and time-consuming samplepreparation, but hyperspectral imaging (HSI) and laser...master thesis 2023
- document
-
Spengler, Daniel (author)The study of tumor microenvironments (TMEs) and immune cell composition in cancer, a disease characterized by uncontrolled growth and spread of tumor cells, has become increasingly important for understanding tumor progression and patient outcomes. Tools such as the TME-Analyzer enable this kind of research, but their manual workflows highlight...master thesis 2023
- document
-
Hoogland, Maxime (author)CO2 electrolysis to form CO with the use of electricity from renewable sources is a promising technique in closing the carbon cycle. Via the electrochemical reduction CO2 can be converted into different hydrocarbon products. These value-added products are for example CO, HCOOH, CH3OH or C2H4. The direct electrochemical route of CO2 reduction is...master thesis 2023
- document
-
Nagalingam, Nagaraj (author), Raghunathan, Aswin (author), Korede, V.B. (author), Overmars, E.F.J. (author), Hung, S. (author), Hartkamp, Remco (author), Padding, J.T. (author), Smith, C.S. (author), Eral, H.B. (author)Optofluidic devices have revolutionized the manipulation and transportation of fluid at smaller length scales ranging from micrometers to millimeters. We describe a dedicated optical setup for studying laser-induced cavitation inside a microchannel. In a typical experiment, we use a tightly focused laser beam to locally evaporate the solution...journal article 2023
- document
-
Sharaf, A.M.S.E. (author), Frimat, J. P. (author), Kremers, G. J. (author), Accardo, A. (author)Two-photon polymerization (2PP) has provided the field of cell biology with the opportunity to fabricate precisely designed microscaffolds for a wide range of studies, from mechanobiology to in vitro disease modelling. However, a multitude of commercial and in-house developed photosensitive materials employed in 2PP suffers from high auto...journal article 2023
- document
-
Mc Cluskey, K.A. (author), Dekker, N.H. (author)TIRF (Total Internal Reflection Fluorescence) microscopy is a powerful tool for measuring the intra- and intermolecular dynamics of fluorescently-labeled single molecules. As TIRF measurements move to more complex biological systems with more fluorescent probes, the multi-band-pass dichroic that separates excitation from emission becomes...journal article 2023
- document
-
Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...journal article 2023
- document
-
Bleeker, J.J. (author), Kahn, A.P. (author), Baumgartner, L.M. (author), Grozema, F.C. (author), Vermaas, D.A. (author), Jager, W.F. (author)Spatiotemporal pH imaging using fluorescence lifetime imaging microscopy (FLIM) is an excellent technique for investigating dynamic (electro)chemical processes. However, probes that are responsive at high pH values are not available. Here, we describe the development and application of dedicated pH probes based on the 1-methyl-7-amino...journal article 2023
- document
-
Baumgartner, L.M. (author), Kahn, A.P. (author), Hoogland, Maxime (author), Bleeker, J.J. (author), Jager, W.F. (author), Vermaas, D.A. (author)Electrochemical CO<sub>2</sub> reduction poses a promising pathway to produce hydrocarbon chemicals and fuels without relying on fossil fuels. Gas diffusion electrodes allow high selectivity for desired carbon products at high current density by ensuring a sufficient CO<sub>2</sub> mass transfer rate to the catalyst layer. In addition to CO...journal article 2023
- document
-
Neves Sao Pedro, M. (author), Isaksson, Madelène (author), Gomis-Fons, Joaquín (author), Eppink, Michel H.M. (author), Nilsson, Bernt (author), Ottens, M. (author)The implementation of continuous processing in the biopharmaceutical industry is hindered by the scarcity of process analytical technologies (PAT). To monitor and control a continuous process, PAT tools will be crucial to measure real-time product quality attributes such as protein aggregation. Miniaturizing these analytical techniques can...journal article 2023
- document
-
Fan, D. (author), Cnossen, J.P. (author), Hung, S. (author), Kromm, D. (author), Dekker, N.H. (author), Verbiest, G.J. (author), Smith, C.S. (author)To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial resolution of microscopes however, is a limiting factor in fluorescence imaging, correspondingly in fluorescence based single molecule localization microscopy (SMLM). Here, we present...journal article 2023
- document
-
de Almeida Nieto, L.M. (author), Gabrieli, Francesca (author), van Loon, A. (author), Gonzalez, Victor (author), Dik, J. (author), Van de Plas, Raf (author), Alfeld, M.W.E.M. (author)Macroscopic x-ray fluorescence imaging spectroscopy (MA-XRF) and reflectance imaging spectroscopy (RIS) are important tools in the analysis of cultural heritage objects, both for conservation and art historical research purposes. The elemental and molecular distributions provided by MA-XRF and RIS respectively, are particularly useful for the...journal article 2023
- document
-
Tummers, Fokkedien H.M.P. (author), Bazelmans, Maria K. (author), Jansen, F.W. (author), Blikkendaal, Mathijs D. (author), Vahrmeijer, Alexander L. (author), Kuppen, P.J.K. (author)Intra-operative fluorescent imaging of endometriosis could help to optimize surgical treatment. Potential biomarkers to use as target for endometriosis-binding fluorescent probes were identified using a new five-phase transcriptomics-based approach to broaden the search for biomarkers. Using publicly available datasets, a differentially...review 2023