Searched for: subject:"fluorescence%5C+microscopy"
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Deb Roy, S. (author)
The basis of this thesis has been the curiosity, however modest, to understand how DNA replication happens in vivo, particularly during the onset of DNA damage and beyond. DNA damage is a recurring phenomenon, which a (bacterial) cell faces in its lifetime from the environment or even its inherent metabolism. While we understand much about...
doctoral thesis 2020
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Wong, H.S. (author), Poole, A.B. (author), Wells, B. (author), Eden, M. (author), Barnes, R. (author), Ferrari, J. (author), Fox, R. (author), Yio, M.H.N. (author), Copuroglu, Oguzhan (author)
Water to cement (w/c) ratio is usually the most important parameter specified in concrete design and is sometimes the subject of dispute when a shortfall in concrete strength or durability is an issue. However, determination of w/c ratio in hardened concrete by testing is very difficult once the concrete has set. This paper presents the...
journal article 2020
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Noguera López, J. (author)
The creation of artificial cells with the minimal set of components to exhibit self-maintenance, self-reproducibility and evolvability (in other words, to be considered alive) is one of the most exciting areas within the field of synthetic biology. Such entities, here called minimal cells, are constructed by either the top-down or bottom-up...
doctoral thesis 2019
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Beekers, D.I. (author), Lattwein, Kirby R. (author), Kouijzer, Joop J.P. (author), Langeveld, Simone A.G. (author), Vegter, M. (author), Beurskens, Robert (author), Mastik, F. (author), Verduyn Lunel, Rogier (author), van der Steen, A.F.W. (author), de Jong, N. (author)
Controlling microbubble-mediated drug delivery requires the underlying biological and physical mechanisms to be unraveled. To image both microbubble oscillation upon ultrasound insonification and the resulting cellular response, we developed an optical imaging system that can achieve the necessary nanosecond temporal and nanometer spatial...
journal article 2019
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Ha, S. (author)
This thesis describes developments in the single-molecule instrumentation, in particular optical torque wrench and DNA nanocurtains, with the goal of employing these techniques in studies of biomolecules and biomotors. Importantly, the use of single-crystal rutile titanium dioxide nanocylinders is suggested for optical torque wrench, enabling...
doctoral thesis 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...
conference paper 2018
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Ando, Toshio (author), Bhamidimarri, Satya Prathyusha (author), Brending, Niklas (author), Colin-York, H. (author), Collinson, Lucy (author), De Jonge, Niels (author), De Pablo, P. J. (author), Debroye, Elke (author), Eggeling, Christian (author), Franck, Christian (author), Hoogenboom, J.P. (author)
Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x...
review 2018
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Gritti, N. (author)
doctoral thesis 2017
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Tiruvadi Krishnan, S. (author)
All living organisms pass on their genetic information to their offspring in the form of DNA or RNA molecules by duplicating them across generations. In the bacteria, their genes are packed in long chains of DNA molecules or chromosomes. One of the widely studied model organisms, Escherichia coli, replicates its circular chromosome in two...
doctoral thesis 2016
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De Martino, M. (author)
doctoral thesis 2016
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Moerland, R.J. (author), Hoogenboom, J.P. (author)
Available data: Complex refractive index of Indium Tin Oxide, http://dx.doi.org/10.4121/uuid:59febf27-a532-4ac9-8ec0-29d4195b2c8c Transparent conductive oxides (TCOs), such as the well-known indium-tin oxide, find widespread use in modern (nano)technological applications because of their unique combination of negligible optical absorption and...
journal article 2016
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Chakrova, N. (author), Rieger, B. (author), Stallinga, S. (author)
We present a versatile fluorescence microscope, built by complementing a conventional fluorescence microscope with a digital micro-mirror device (DMD) in the illumination path. Arbitrary patterns can be created on the DMD and projected onto the sample. This patterned illumination can be used to improve lateral and axial resolution over the...
conference paper 2015
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Nieuwenhuizen, R.P.J. (author), Nahidiazar, L. (author), Manders, E.M.M. (author), Jalink, K. (author), Stallinga, S. (author), Rieger, B. (author)
Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of...
journal article 2015
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)
Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average number of localizations per fluorophore, or...
journal article 2015
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Burri, S. (author), Maruyama, Y. (author), Michalet, X. (author), Regazzoni, F. (author), Bruschini, C. (author), Charbon, E. (author)
We present the architecture and three applications of the largest resolution image sensor based on single-photon avalanche diodes (SPADs) published to date. The sensor, fabricated in a high-voltage CMOS process, has a resolution of 512 × 128 pixels and a pitch of 24 ?m. The fill-factor of 5% can be increased to 30% with the use of microlenses....
journal article 2014
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Broeken, J. (author), Rieger, B. (author), Stallinga, S. (author)
We propose a method for simultaneously measuring the position and emission color of single fluorescent emitters based on the use of a large pitch diffraction grating in the emission light path. The grating produces satellite spots adjacent to the main spot; the relative distance between the spots is a measure for the emission wavelength. We...
journal article 2014
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Righolt, C.H. (author), Mai, S. (author), Van Vliet, L.J. (author), Stallinga, S. (author)
The quality of the reconstructed image in structured illumination microscopy (SIM) depends on various aspects of the image filtering process. To optimize the trade-off between resolution and ringing artifacts, which lead to negative intensities, we extend Lukosz-bound filtering to 3D SIM and derive the parametrization of the 3D SIM cut-off. We...
journal article 2014
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Franken, M.J.Z. (author)
Despite the fact that moving droplets are very common, a moving contact line is a longstanding fundamental challenge in the field of fluid mechanics. This fundamental challenge is the main topic of this thesis in the context of ASML immersion lithography equipment. In Chapter 2 an overview of existing theoretical and experimental work is given,...
doctoral thesis 2014
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Meddens, M.B.M. (author), Rieger, B. (author), Figdor, C.G. (author), Cambi, A. (author), Van den Dries, K. (author)
Podosomes are cellular adhesion structures involved in matrix degradation and invasion that comprise an actin core and a ring of cytoskeletal adaptor proteins. They are most often identified by staining with phalloidin, which binds F-actin and therefore visualizes the core. However, not only podosomes, but also many other cytoskeletal structures...
journal article 2013
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Valcke, S.L.A. (author), Polder, R.B. (author), Beijersbergen van Henegouwen, J.F. (author)
conference paper 2012
Searched for: subject:"fluorescence%5C+microscopy"
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