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Groot, C. (author), Larbi, J. (author)Water loss from the fresh mortar is believed to be related to mortar-brick bond strength development in masonry. Recent research on mortar-brick bond has shown that, particularly, effects of water flow on the composition and the hydration conditions of the mortar-brick interface have to be taken into account to explain bond strength development....journal article 1999
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Young, I.T. (author), Garini, Y. (author), Dietrich, H.R.C. (author), Van Oel, W. (author), Liqui Lung, G. (author)Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to...conference paper 2004
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Dietrich, H.R.C. (author), Van den Doel, R.L. (author), Hoyer, W. (author), Van Oel, W. (author), Liqui Lung, G. (author), Garini, Y. (author), Jovin, T. (author), Young, I.T. (author)In previous publications we have shown that we can perform enzymatic reactions in nanoarrays by means of a microarray-reader based on a conventional microscope. In this publication we report on a modification of this system in order to monitor the aggregation kinetics of the natively unfolded protein ?-synuclein. We describe the motivation for...conference paper 2005
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Vermolen, B.J. (author), Young, I.T. (author), Chuang, A. (author), Wark, L. (author), Chuang, T. (author), Mai, S. (author), Garini, Y. (author)Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we...conference paper 2005
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Young, I.T. (author), Garini, Y. (author), Vermolen, B. (author), Liqui Lund, G. (author), Brouwer, G. (author), Hendrichs, S. (author), El Morabit, M. (author), Spoelstra, J. (author), Wilhelm, E. (author), Zaal, M. (author)While fluorescence microscope systems remains an essential tool in modern biology and medical work, no compact instrumentation has been developed for the rapid calibration of such systems. Almost invariably results are presented in terms of the [AU], "arbitrary units". To remedy this situation we have developed a small, portable instrument - the...conference paper 2006
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Hagen, G.M. (author), Caarls, W. (author), Thomas, M. (author), Hill, A. (author), Lidke, K.A. (author), Rieger, B. (author), Fritsch, C. (author), Van Geest, B. (author), Jovin, T.M. (author), Arndt-Jovin, D.J. (author)We report on a new generation, commercial prototype of a programmable array optical sectioning fluorescence microscope (PAM) for rapid, light efficient 3D imaging of living specimens. The stand-alone module, including light source(s) and detector(s), features an innovative optical design and a ferroelectric liquid-crystal-on-silicon (LCoS)...conference paper 2007
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Young, I.T. (author), El Morabit, M. (author), Liqui Lung, G. (author), Vermolen, B.J. (author)Based upon a collection of compact LEDs (light-emitting diodes) and a compact photodiode, we have developed a calibration tool for fluorescence microscopes that are used as digital imaging devices. The entire device (excluding a USB connector) measures 25 mm x 80 mm x 12 mm. Virtually all commonly-used fluorophores can be simulated with one of...conference paper 2008
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Li, D.U. (author), Arlt, J. (author), Richardson, J. (author), Walker, R. (author), Buts, A. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author)A compact real-time fluorescence lifetime imaging microscopy (FLIM) system based on an array of low dark count 0.13?m CMOS singlephoton avalanche diodes (SPADs) is demonstrated. Fast background-insensitive fluorescence lifetime determination is achieved by use of a recently proposed algorithm called ‘Integration for Extraction Method’ (IEM) [J....journal article 2010
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Giraud, G. (author), Schulze, H. (author), Li, D.U. (author), Bachmann, T.T. (author), Crain, J. (author), Tyndall, D. (author), Richardson, J. (author), Walker, R. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author), Arlt, J. (author)Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires...journal article 2010
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Stallinga, S. (author), Rieger, B. (author)The Gaussian function is simple and easy to implement as Point Spread Function (PSF) model for fitting the position of fluorescent emitters in localization microscopy. Despite its attractiveness the appropriateness of the Gaussian is questionable as it is not based on the laws of optics. Here we study the effect of emission dipole orientation in...journal article 2010
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Zhao, Q. (author), Young, I.T. (author), De Jong, J.G.S. (author)We have constructed a mathematical model to analyze the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy (FLIM). The power of the light source needed for illumination in a FLIM system and the signal-to-noise ratio of the detector have led us to a photon “budget.” These measures are relevant to many fluorescence...journal article 2011
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Stallinga, S. (author), Rieger, B. (author)We introduce a method for determining the position and orientation of fixed dipole emitters based on a combination of polarimetry and spot shape detection. A key element is an effective Point Spread Function model based on Hermite functions. The model offers a good description of the shape variations with dipole orientation and polarization...journal article 2012
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Huang, Z. (author)Heterogeneity is a general feature in biological system. In order to avoid possible misleading effects of ensemble averaging, and to ensure a correct understanding of the biological system, it is very important to look into individuals, such as a single bio-molecule or a single cell, for details. The size of a single bio-molecule/cell typically...doctoral thesis 2012
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- Valcke, S.L.A. (author), Polder, R.B. (author), Beijersbergen van Henegouwen, J.F. (author) conference paper 2012
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Meddens, M.B.M. (author), Rieger, B. (author), Figdor, C.G. (author), Cambi, A. (author), Van den Dries, K. (author)Podosomes are cellular adhesion structures involved in matrix degradation and invasion that comprise an actin core and a ring of cytoskeletal adaptor proteins. They are most often identified by staining with phalloidin, which binds F-actin and therefore visualizes the core. However, not only podosomes, but also many other cytoskeletal structures...journal article 2013
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Franken, M.J.Z. (author)Despite the fact that moving droplets are very common, a moving contact line is a longstanding fundamental challenge in the field of fluid mechanics. This fundamental challenge is the main topic of this thesis in the context of ASML immersion lithography equipment. In Chapter 2 an overview of existing theoretical and experimental work is given,...doctoral thesis 2014
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Righolt, C.H. (author), Mai, S. (author), Van Vliet, L.J. (author), Stallinga, S. (author)The quality of the reconstructed image in structured illumination microscopy (SIM) depends on various aspects of the image filtering process. To optimize the trade-off between resolution and ringing artifacts, which lead to negative intensities, we extend Lukosz-bound filtering to 3D SIM and derive the parametrization of the 3D SIM cut-off. We...journal article 2014
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Broeken, J. (author), Rieger, B. (author), Stallinga, S. (author)We propose a method for simultaneously measuring the position and emission color of single fluorescent emitters based on the use of a large pitch diffraction grating in the emission light path. The grating produces satellite spots adjacent to the main spot; the relative distance between the spots is a measure for the emission wavelength. We...journal article 2014
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Burri, S. (author), Maruyama, Y. (author), Michalet, X. (author), Regazzoni, F. (author), Bruschini, C. (author), Charbon, E. (author)We present the architecture and three applications of the largest resolution image sensor based on single-photon avalanche diodes (SPADs) published to date. The sensor, fabricated in a high-voltage CMOS process, has a resolution of 512 × 128 pixels and a pitch of 24 ?m. The fill-factor of 5% can be increased to 30% with the use of microlenses....journal article 2014
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)Quantification in localization microscopy with reversibly switchable fluorophores is severely hampered by the unknown number of switching cycles a fluorophore undergoes and the unknown stoichiometry of fluorophores on a marker such as an antibody. We overcome this problem by measuring the average number of localizations per fluorophore, or...journal article 2015