Searched for: subject%3A%22metabolomics%22
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Cueto Rojas, H.F. (author), Milne, Nicholas (author), van Helmond, W. (author), Pieterse, M.M. (author), Maris, Antonius J A (author), Daran, J.G. (author), Wahl, S.A. (author)
Background: Microbial production of nitrogen containing compounds requires a high uptake flux and assimilation of the N-source (commonly ammonium), which is generally coupled with ATP consumption and negatively influences the product yield. In the industrial workhorse Saccharomyces cerevisiae, ammonium (NH<sub>4</sub> <sup>+</sup>) uptake is...
journal article 2017
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Cueto Rojas, H.F. (author), Maleki Seifar, R. (author), ten Pierick, A. (author), Heijnen, J.J. (author), Wahl, S.A. (author)
Ammonium (NH4+) is the most common N-source for yeast fermentations, and N-limitation is frequently applied to reduce growth and increase product yields. While there is significant molecular knowledge on NH4 + transport and assimilation, there have been few attempts to measure the in vivo concentration of this metabolite. In this article, we...
journal article 2016
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Lu, Hongzhong (author), Liu, X. (author), Huang, Mingzhi (author), Xia, Jianye (author), Chu, Ju (author), Zhuang, Yingping (author), Zhang, Siliang (author), Noorman, H.J. (author)
Background: Aspergillus niger is widely used for enzyme production and achievement of high enzyme production depends on the comprehensive understanding of cell's metabolic regulation mechanisms. Results: In this paper, we investigate the metabolic differences and regulation mechanisms between a high glucoamylase-producing strain A. niger...
journal article 2015
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Lameiras, F. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)
In view of the high citric acid production capacity of Aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. Quantitative metabolomics is an important omics tool in a synthetic biology approach to develop A. niger for the production of these acids. Such...
journal article 2015
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Jorda, J. (author), Cueto Rojas, H.F. (author), Carnicer, M. (author), Wahl, S.A. (author), Ferrer, P. (author), Albiol, J. (author)
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. pastoris central carbon metabolism....
journal article 2014
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Cruz, L.A.B. (author), Hebly, M. (author), Duong, G.H. (author), Wahl, S.A. (author), Pronk, J.T. (author), Heijnen, J.J. (author), Daran-Lapujade, P. (author), Van Gulik, W.M. (author)
Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes....
journal article 2012
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De Jonge, L.P. (author), Douma, R.D. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)
A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite leakage during sample treatment. The optimization study included amino acids and intermediates of the glycolysis and the TCA-cycle. Metabolite leakage was found to be minimal...
journal article 2011
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Taymaz Nikerel, H. (author)
Kinetic models, which predict the behaviour of metabolic reaction networks under different conditions, are indispensible to fully and quantitatively understand the relation between a product pathway and connected central metabolism. In this thesis the focus was to develop tools for future in vivo kinetic modeling in Escherichia coli. The...
doctoral thesis 2010
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Canelas, A.B. (author), Ras, C. (author), Ten Pierick, A. (author), Van Dam, J.C. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)
Accurate determination of intracellular metabolite levels requires reliable, reproducible techniques for sampling and sample treatment. Quenching in 60% (v/v) methanol at ?40°C is currently the standard method for sub-second arrest of metabolic activity in microbial metabolomics but there have been contradictory reports in the literature on...
journal article 2008
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Nasution, U.D.M. (author)
Abstract not available
doctoral thesis 2007
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Mashego, M.R. (author), Rumbold, K. (author), De Mey, M. (author), Vandamme, E. (author), Soetaert, W. (author), Heijnen, J.J. (author)
journal article 2007
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Mashego, M.R. (author)
In this thesis, robust experimental methods and experimental designs for quantitative analysis of the metabolome (concentrations of relevant metabolites in the cell) of Saccharomyces cerevisiae (Bakers yeast) have been presented. In this research, it is essential to obtain a snapshot of intracellular as well as extracellular metabolite...
doctoral thesis 2005
Searched for: subject%3A%22metabolomics%22
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