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Giraud, G. (author), Schulze, H. (author), Li, D.U. (author), Bachmann, T.T. (author), Crain, J. (author), Tyndall, D. (author), Richardson, J. (author), Walker, R. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author), Arlt, J. (author)
Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires...
journal article 2010
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Li, D.U. (author), Arlt, J. (author), Richardson, J. (author), Walker, R. (author), Buts, A. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author)
A compact real-time fluorescence lifetime imaging microscopy (FLIM) system based on an array of low dark count 0.13?m CMOS singlephoton avalanche diodes (SPADs) is demonstrated. Fast background-insensitive fluorescence lifetime determination is achieved by use of a recently proposed algorithm called ‘Integration for Extraction Method’ (IEM) [J....
journal article 2010