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Antolović, I.M. (author), Burri, S (author), Hoebe, RA (author), Maruyama, Yuki (author), Bruschini, C (author), Charbon-Iwasaki-Charbon, E. (author)The paper presents a camera comprising 512 × 128 pixels capable of single-photon detection and gating with a maximum frame rate of 156 kfps. The photon capture is performed through a gated single-photon avalanche diode that generates a digital pulse upon photon detection and through a digital one-bit counter. Gray levels are obtained through...journal article 2016
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Zhao, Q. (author)Fluorescence microscopy is a well-established platform for biology and biomedical research (Chapter 2). Based on this platform, fluorescence lifetime imaging microscopy (FLIM) has been developed to measure fluorescence lifetimes, which are independent of fluorophore concentration and excitation intensity and offer more information about the...doctoral thesis 2014
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Zhao, Q. (author), Schelen, B. (author), Schouten, R. (author)We have built an all-solid-state camera that is directly modulated at the pixel level for frequency-domain fluorescence lifetime imaging microscopy (FLIM) measurements. This novel camera eliminates the need for an image intensifier through the use of an application-specific charge coupled device design in a frequency-domain FLIM system. The...journal article 2012
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Li, D.D.U. (author), Arlt, J. (author), Tyndall, D. (author), Walker, R. (author), Richardson, J. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R.K. (author)A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 × 32 low dark count 0.13 ?m complementary metaloxide-semiconductor single-photon...journal article 2011
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Zhao, Q. (author), Young, I.T. (author), De Jong, J.G.S. (author)We have constructed a mathematical model to analyze the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy (FLIM). The power of the light source needed for illumination in a FLIM system and the signal-to-noise ratio of the detector have led us to a photon “budget.” These measures are relevant to many fluorescence...journal article 2011