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Noom, J. (author), Soloviev, O.A. (author), Smith, C.S. (author), Nguyen, Hieu Thao (author), Verhaegen, M.H.G. (author)We demonstrate a novel closed-loop input design technique on the detection of particles in an imaging system such as a fluorescence microscope. The probability of misdiagnosis is minimized while constraining the input energy such that for instance phototoxicity is reduced. The key novelty of the closed-loop design is that each next input is...conference paper 2022
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Kamat, Ameya (author), Lubelli, B. (author), Schlangen, E. (author)Sodium chloride (NaCl) is one of the ubiquitous soluble salts in the environment and is responsible for weathering of building materials. The salt weathering is attributed to the stress developed from crystallisation of these salts in pores of the building materials, with supersaturation as the driving force. In the last years, researchers have...conference paper 2021
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van Kessel, L.C.P.M. (author), Huisman, T. (author), Hagen, C.W. (author)Line-edge roughness (LER) is often measured from top-down critical dimension scanning electron microscope (CD-SEM) images. The true three-dimensional roughness profile of the sidewall is typically ignored in such analyses. We study the response of a CD-SEM to sidewall roughness (SWR) by simulation. We generate random rough lines and spaces,...conference paper 2020
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Karuna, A. (author), Masia, Francesco (author), Wiltshire, Marie (author), Errington, Rachel (author), Borri, Paola (author), Langbein, Wolfgang (author)Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free,...conference paper 2019
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Vigueras Guillén, J.P. (author), Lemij, Hans G. (author), Van Rooij, Jeroen (author), Vermeer, K.A. (author), van Vliet, L.J. (author)In images of the corneal endothelium (CE) acquired by specular microscopy, endothelial cells are commonly only visible in a part of the image due to varying contrast, mainly caused by challenging imaging conditions as a result of a strongly curved endothelium. In order to estimate the morphometric parameters of the corneal endothelium, the...conference paper 2019
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van Kessel, L.C.P.M. (author), Hagen, C.W. (author), Kruit, P. (author)We have investigated the contributions of surface effects to Monte Carlo simulations of top-down scanning electron microscopy (SEM) images. The elastic and inelastic scattering mechanisms in typical simulations assume that the electron is deep in the bulk of the material. In this work, we correct the inelastic model for surface effects. We...conference paper 2019
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Quintavalla, M. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author), Bijlsma, H.K. (author), Verstraete, H.R.G.W. (author), Bonora, S. (author)Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL)...conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...conference paper 2018
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...conference paper 2018
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van der Graaff, L. (author), Boyaval, Fanny (author), van Vliet, L.J. (author), Stallinga, S. (author)In the field of pathology there is an ongoing transition to the use of Whole Slide Imaging (WSI) systems which scan tissue slides at intermediate resolution (0∼.25 μm) and high throughput (15mm<sup>2</sup>=min) to digital image files. Most scanners currently on the market are line-sensor based push-broom scanners for three-color (RGB)...conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...conference paper 2018
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Vigueras Guillén, J.P. (author), Engel, Angela (author), Lemij, Hans G. (author), van Rooij, Jeroen (author), Vermeer, K.A. (author), van Vliet, L.J. (author)Clinical parameters related to the corneal endothelium can only be estimated by segmenting endothelial cell images. Specular microscopy is the current standard technique to image the endothelium, but its low SNR make the segmentation a complicated task. Recently, we proposed a method to segment such images by starting with an oversegmented...conference paper 2018
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de Rooij, Mario R. (author), França de Mendonça Filho, F. (author)In this article a trip is taken to characterize concrete through its hardened cement paste based on its microstructure. Optical microscopy is coupled to advanced image analysis, as well as statistical data analysis to characterize heterogeneous concrete material. The article takes a closer look at the determination of the w/c ratio through the...conference paper 2017
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Bolten, Jens (author), Arat, K.T. (author), Ünal, Nezih (author), Porschatis, Caroline (author), Wahlbrink, Thorsten (author), Lemme, Max C. (author)In this paper key challenges posed on metrology by feature dimensions of 20nm and below are discussed. In detail, the need for software-based tools for SEM image acquisition and image analysis in environments where CD-SEMs are not available and/or not flexible enough to cover all inspection tasks is outlined. These environments include...conference paper 2017
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van der Walle, P (author), Kramer, E. (author), van der Donck, J.C.J. (author), Mulckhuyse, W (author), Nijsten, L. (author), Bernal Arango, F.A. (author), de Jong, A. (author), van Zeijl, E. (author), Spruit, H. E.T. (author), van den Berg, J.H. (author), Nanda, G. (author), van Langen-Suurling, A.K. (author), Alkemade, P.F.A. (author), Pereira, S.F. (author), Maas, D.J. (author)Particle defects are important contributors to yield loss in semi-conductor manufacturing. Particles need to be detected and characterized in order to determine and eliminate their root cause. We have conceived a process flow for advanced defect classification (ADC) that distinguishes three consecutive steps; detection, review and...conference paper 2017
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Nahar, S.N. (author), Schmets, A.J.M. (author), Kasbergen, C. (author), Schitter, G (author), Scarpas, Athanasios (author)Self-healing of bitumen is a property that positively contributes to the sustainability, maintenance requirements and cost effectiveness of asphalt pavements. Ideally one would like to design an asphalt mix with a well-defined healing potential. Although substantial research efforts have been dedicated to the healing mechanism in bitumen,...conference paper 2016
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Yoo, H.W. (author), van Royen, M.E. (author), van Cappellen, WA (author), Houtsmuller, AB (author), Verhaegen, M.H.G. (author), Schitter, G (author)The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with...conference paper 2016
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...conference paper 2016
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in...conference paper 2016
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Bijster, R.J.F. (author), Sadeghian Marnani, H. (author), van Keulen, A. (author)Optical near-field technologies such as solid immersion lenses and hyperlenses are candidate solutions for high resolution and high throughput wafer inspection and metrology for the next technology nodes. Besides sub-diffraction limited optical performance, these concepts share the necessity of extreme proximity to the sample at distances that...conference paper 2016