Searched for: subject:"microscopy"
(1 - 20 of 260)

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Hagen, G.M. (author), Caarls, W. (author), Thomas, M. (author), Hill, A. (author), Lidke, K.A. (author), Rieger, B. (author), Fritsch, C. (author), Van Geest, B. (author), Jovin, T.M. (author), Arndt-Jovin, D.J. (author)
We report on a new generation, commercial prototype of a programmable array optical sectioning fluorescence microscope (PAM) for rapid, light efficient 3D imaging of living specimens. The stand-alone module, including light source(s) and detector(s), features an innovative optical design and a ferroelectric liquid-crystal-on-silicon (LCoS)...
conference paper 2007
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Maas, D. (author), Van Veldhoven, E. (author), Chen, P. (author), Sidorkin, V. (author), Salemink, H. (author), Van der Drift, E. (author), Alkemade, P. (author)
The recently introduced helium ion microscope (HIM) is capable of imaging and fabrication of nanostructures thanks to its sub-nanometer sized ion probe [1,2]. The unique interaction of the helium ions with the sample material provides very localized secondary electron emission, thus providing a valuable signal for high-resolution imaging as well...
conference paper 2009
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Müller, M. (author), Schins, J.M. (author), Wurpel, G.W.H. (author)
In multiplex CARS microscopy the generated anti-Stokes signal is generated and detected simultaneously over a significant part of the vibrational spectrum. The signal-to-noise ratio of the thus detected spectra is limited only by shot-noise. This principle is demonstrated using a dilution series of 2-propanol in water. It is derived...
conference paper 2004
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Stallinga, S. (author)
Localization microscopy for imaging at the nano-scale relies on the quality of fitting the emitter positions from the measured light spots. The type and magnitude of the noise in the detection process, the background light level and the Point Spread Function model that is used in the fit are of paramount importance for the precision and accuracy...
conference paper 2015
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Young, I.T. (author), Garini, Y. (author), Vermolen, B. (author), Liqui Lund, G. (author), Brouwer, G. (author), Hendrichs, S. (author), El Morabit, M. (author), Spoelstra, J. (author), Wilhelm, E. (author), Zaal, M. (author)
While fluorescence microscope systems remains an essential tool in modern biology and medical work, no compact instrumentation has been developed for the rapid calibration of such systems. Almost invariably results are presented in terms of the [AU], "arbitrary units". To remedy this situation we have developed a small, portable instrument - the...
conference paper 2006
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Bolten, Jens (author), Arat, K.T. (author), Ünal, Nezih (author), Porschatis, Caroline (author), Wahlbrink, Thorsten (author), Lemme, Max C. (author)
In this paper key challenges posed on metrology by feature dimensions of 20nm and below are discussed. In detail, the need for software-based tools for SEM image acquisition and image analysis in environments where CD-SEMs are not available and/or not flexible enough to cover all inspection tasks is outlined. These environments include...
conference paper 2017
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Quintavalla, M. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author), Verstraete, H.R.G.W. (author), Bonora, S. (author)
Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL)...
conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, Gleb (author), Verhaegen, M.H.G. (author)
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...
conference paper 2016
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Van der Velde, O. (author), Copuroglu, O. (author), Veer, F.A. (author)
This paper shows the benefit of using transmitted light microscopy together with a Z-scanning software in fractographical analyses of glass. The strength of glass is largely dependent on processes that happen at the microscale. In this research, 52 plates were fractured in a biaxial tensile test. These were divided into five categories according...
conference paper 2015
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Young, I.T. (author), Garini, Y. (author), Dietrich, H.R.C. (author), Van Oel, W. (author), Liqui Lung, G. (author)
Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to...
conference paper 2004
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovine, Gleb (author), Verhaegen, M.H.G. (author)
This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in...
conference paper 2016
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Brancart, J. (author), Scheltjes, G. (author), Diaz, M.M. (author), Van Mele, B. (author), Van Assche, G. (author)
Research in the field of smart materials that exhibit self-repair mechanisms has greatly expanded over the last few years. This is especially true for polymers and polymer composite materials. One class of self-healing polymer materials is the reversible polymer network systems that use dynamic covalent bonds as a means to repair sustained...
conference paper 2013
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Maas, D. (author), Van veldhoven, E. (author), Chen, P. (author), Sidorkin, V. (author), Salemink, H. (author), Van der Drift, E. (author), Alkemade, P. (author)
The recently introduced helium ion microscope (HIM) is capable of imaging and fabrication of nanostructures thanks to its sub-nanometer sized ion probe [1,2]. The unique interaction of the helium ions with the sample material provides very localized secondary electron emission, thus providing a valuable signal for high-resolution imaging as well...
conference paper 2010
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Markovich, I. (author), Van Mier, J.G.M. (author), Walraven, J.C. (author)
Hybrid fiber reinforcement can be very efficient for improving the tensile response of the composite. In such materials, fibers of different geometries can act as bridging mechanisms over cracks of different widths. The fiber bridging efficiency depends on the interface properties, which makes interface characterization very important. Therefore...
conference paper 2001
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Sadeghian Marnani, H. (author), Herfst, R.W. (author), Van den Dool, T.C. (author), Crowcombe, W.E. (author), Winters, J. (author), Kramer, G.F.I.J. (author)
Scanning probe microscopy (SPM) is a promising candidate for accurate assessment of metrology and defects on wafers and masks, however it has traditionally been too slow for high-throughput applications, although recent developments have significantly pushed the speed of SPM [1,2]. In this paper we present new results obtained with our...
conference paper 2014
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Dietrich, H.R.C. (author), Van den Doel, R.L. (author), Hoyer, W. (author), Van Oel, W. (author), Liqui Lung, G. (author), Garini, Y. (author), Jovin, T. (author), Young, I.T. (author)
In previous publications we have shown that we can perform enzymatic reactions in nanoarrays by means of a microarray-reader based on a conventional microscope. In this publication we report on a modification of this system in order to monitor the aggregation kinetics of the natively unfolded protein ?-synuclein. We describe the motivation for...
conference paper 2005
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Chakrova, N. (author), Rieger, B. (author), Stallinga, S. (author)
We present a versatile fluorescence microscope, built by complementing a conventional fluorescence microscope with a digital micro-mirror device (DMD) in the illumination path. Arbitrary patterns can be created on the DMD and projected onto the sample. This patterned illumination can be used to improve lateral and axial resolution over the...
conference paper 2015
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, Gleb (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...
conference paper 2018
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Valcke, S.L.A. (author), Polder, R.B. (author), Beijersbergen van Henegouwen, J.F. (author)
conference paper 2012
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Gersbach, M. (author), Trimananda, R. (author), Maruyama, Y. (author), Fishburn, M. (author), Cahrbon, E. et al (author)
Imaging techniques based on time-correlated single photon counting (TCSPC), such as fluorescence lifetime imaging microscopy (FLIM), rely on fast single-photon detectors as well as timing electronics in the form of time-to-digital or time-to-analog converters. Conventional systems rely on stand-alone or small arrays (up to 32) of detectors and...
conference paper 2010
Searched for: subject:"microscopy"
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