Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy

The goal of this thesis, called Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy, concerns itself with the development of new techniques to control adaptive fluorescence microscopes, so that they can adapt and image with increased resolution, contrast and speed inside complex three-dimensional biological...

Hybrid adaptive and computational light-sheet fluorescence microscopy

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...

Three-dimensional structured illumination microscopy using Lukosz bound apodization reduces pixel negativity at no resolution cost

The quality of the reconstructed image in structured illumination microscopy (SIM) depends on various aspects of the image filtering process. To optimize the trade-off between resolution and ringing artifacts, which lead to negative intensities, we extend Lukosz-bound filtering to 3D SIM and derive the parametrization of the 3D SIM cut-off. We...

Nuclear Architecture: Image Processing and Analyses

Cancer is one of the most well-known groups of diseases that finds its cause in cells having chromosomal aberrations. How and why these aberrations can occur is one of the most important questions asked in modern molecular biology. In the last decades it has become clear that gene regulation in the nucleus, where the chromosomes reside, is...