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Tišma, M. (author), Kaljević, Jovana (author), Gruber, Stephan (author), Le, Tung B.K. (author), Dekker, C. (author)Bacterial cells require DNA segregation machinery to properly distribute a genome to both daughter cells upon division. The most common system involved in chromosome and plasmid segregation in bacteria is the ParABS system. A core protein of this system - partition protein B (ParB) - regulates chromosome organization and chromosome...review 2024
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Mc Cluskey, K.A. (author), Dekker, N.H. (author)TIRF (Total Internal Reflection Fluorescence) microscopy is a powerful tool for measuring the intra- and intermolecular dynamics of fluorescently-labeled single molecules. As TIRF measurements move to more complex biological systems with more fluorescent probes, the multi-band-pass dichroic that separates excitation from emission becomes...journal article 2023
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Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...journal article 2023
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Fan, D. (author), Cnossen, J.P. (author), Hung, S. (author), Kromm, D. (author), Dekker, N.H. (author), Verbiest, G.J. (author), Smith, C.S. (author)To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial resolution of microscopes however, is a limiting factor in fluorescence imaging, correspondingly in fluorescence based single molecule localization microscopy (SMLM). Here, we present...journal article 2023
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Chardon, Florian (author), Japaridze, A. (author), Witt, Hannes (author), Velikovsky, Leonid (author), Chakraborty, Camellia (author), Wilhelm, Therese (author), Dumont, Marie (author), Yang, W.W.W. (author), Dekker, C. (author)Chromosome inheritance depends on centromeres, epigenetically specified regions of chromosomes. While conventional human centromeres are known to be built of long tandem DNA repeats, much of their architecture remains unknown. Using single-molecule techniques such as AFM, nanopores, and optical tweezers, we find that human centromeric DNA...journal article 2022
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Kabiri, Y. (author), Angelin, Alessandro (author), Ahmed, Ishtiaq (author), Mutlu, Hatice (author), Bauer, Jens (author), Niemeyer, Christof M. (author), Zandbergen, H.W. (author), Dekker, C. (author)Staining compounds containing heavy elements (electron dyes) can facilitate the visualization of DNA and related biomolecules by using TEM. However, research into the synthesis and utilization of alternative electron dyes has been limited. Here, we report the synthesis of a novel DNA intercalator molecule, bis-acridine uranyl (BAU). NMR...journal article 2019
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Perez, Amilcar J. (author), Cesbron, Yann (author), Shaw, Sidney L. (author), Villicana, Jesus Bazan (author), Tsui, Ho Ching T. (author), Boersma, Michael J. (author), Ye, Ziyun A. (author), Tovpeko, Yanina (author), Dekker, C. (author)Bacterial cell division and peptidoglycan (PG) synthesis are orchestrated by the coordinated dynamic movement of essential protein complexes. Recent studies show that bidirectional treadmilling of FtsZ filaments/bundles is tightly coupled to and limiting for both septal PG synthesis and septum closure in some bacteria, but not in others. Here...journal article 2019
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Kabiri, Y. (author), Ananth, A.N. (author), van der Torre, J. (author), Katan, A.J. (author), Hong, Jin Yong (author), Malladi, S.R.K. (author), Kong, Jing (author), Zandbergen, H.W. (author), Dekker, C. (author)While graphene may appear to be the ultimate support membrane for transmission electron microscopy (TEM) imaging of DNA nanostructures, very little is known if it poses an advantage over conventional carbon supports in terms of resolution and contrast. Microscopic investigations are carried out on DNA origami nanoplates that are supported...journal article 2017
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Herfst, R. (author), Dekker, B. (author), Witvoet, G. (author), Crowcombe, W.E. (author), de Lange, D. (author), Sadeghian Marnani, H. (author)One of the major limitations in the speed of the atomic force microscope (AFM) is the bandwidth of the mechanical scanning stage, especially in the vertical (z) direction. According to the design principles of “light and stiff” and “static determinacy,” the bandwidth of the mechanical scanner is limited by the first eigenfrequency of the AFM...journal article 2015
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Storm, A.J. (author), Chen, J.H. (author), Ling, X.S. (author), Zandbergen, H.W. (author), Dekker, C. (author)The imaging beam of a transmission electron microscope can be used to fine tune critical dimensions in silicon oxide nanostructures. This technique is particularly useful for the fabrication of nanopores with single-nanometer precision, down to 2 nm. We report a detailed study on the effect of electron-beam irradiation on apertures with various...journal article 2005
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LeRoy, B.J. (author), Lemay, S.G. (author), Kong, J. (author), Dekker, C. (author)We have performed low-temperature scanning tunneling microscopy measurements on single-wall carbon nanotubes that are freely suspended over a trench. The nanotubes were grown by chemical vapor deposition on a Pt substrate with predefined trenches etched into it. Atomic resolution was obtained on the freestanding portions of the nanotubes....journal article 2004
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- Venema, L.C. (author), Wildoer, J.W.G. (author), Tuinstra, H.L.J.T. (author), Dekker, C. (author), Rinzler, A.G. (author), Smalley, R.E. (author) journal article 1997
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- Tans, S.J. (author), Geerligs, L.J. (author), Dekker, C. (author), Wu, J. (author), Wegner, G. (author) journal article 1997
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- Van der Zant, H.S.J. (author), Mantel, O.C. (author), Heij, C.P. (author), Dekker, C. (author) journal article 1997
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- Mantel, O.C. (author), Van der Zant, H.S.J. (author), Steinfort, A.J. (author), Traeholt, C. (author), Dekker, C. (author) journal article 1997
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- Dekker, C. (author), Tans, S.J. (author), Oberndorff, B. (author), Meyer, R. (author), Venema, L.C. (author) journal article 1997
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- Dekker, C. (author), Tans, S.J. (author), Geerligs, L.J. (author), Bezryadin, A. (author), Wu, J. (author), Wegner, G. (author) journal article 1997
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- Tans, S.J. (author), Miedema, R. (author), Geerligs, L.J. (author), Dekker, C. (author), Wu, J. (author), Wegner, G. (author) journal article 1997
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- Dekker, J.P. (author), Van der Put, P.J. (author), Veringa, H.J. (author), Schoonman, J. (author) journal article 1994