Searched for: subject%3A%22microscopy%22
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Nutter, John (author), Qi, Jiahui (author), Farahani, H. (author), Rainforth, W. Mark (author), van der Zwaag, S. (author)
The current study reports in situ TEM observations of the growth of bainitic ferrite in an Fe-0.3C-3Mn-1.5Si-0.15Mo steel held isothermally at 300 °C with a higher spatio-temporal resolution than in previous studies. Significant variations were found in the lengthening rate, with the highest being in excess of 30,000 nm.s<sup>−1</sup> while...
journal article 2023
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Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)
Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...
journal article 2023
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Wehrens, Martijn (author), Krah, Laurens H.J. (author), Towbin, Benjamin D. (author), Hermsen, Rutger (author), Tans, S.J. (author)
The inherent stochasticity of metabolism raises a critical question for understanding homeostasis: are cellular processes regulated in response to internal fluctuations? Here, we show that, in E. coli cells under constant external conditions, catabolic enzyme expression continuously responds to metabolic fluctuations. The underlying...
journal article 2023
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Bauer, T.A. (author), Davis, Timothy J. (author), Frank, Bettina (author), Dreher, Pascal (author), Janoschka, David (author), Meiler, Tim C. (author), Meyer zu Heringdorf, Frank J. (author), Kuipers, L. (author), Giessen, Harald (author)
The creation and manipulation of optical vortices, both in free space and in two-dimensional systems such as surface plasmon polaritons (SPPs), has attracted widespread attention in nano-optics due to their robust topological structure. Coupled with strong spatial confinement in the case of SPPs, these plasmonic vortices and their underlying...
journal article 2023
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de Rooij, Jimmy (author), Vintges, M.Q. (author), Zuidwijk, T. (author), Heerkens, C.T.H. (author), Schulp, Anne S. (author)
Identification of ontogenetic age classes plays an important role in the fields of zoology, palaeontology and archaeology, where accurate age classifications of (sub)fossil remains are a crucial component for the reconstruction of past life. Textural ageing—the identification of age-related bone surface textures—provides a size-independent...
journal article 2023
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Fan, D. (author), Cnossen, J.P. (author), Hung, S. (author), Kromm, D. (author), Dekker, N.H. (author), Verbiest, G.J. (author), Smith, C.S. (author)
To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial resolution of microscopes however, is a limiting factor in fluorescence imaging, correspondingly in fluorescence based single molecule localization microscopy (SMLM). Here, we present...
journal article 2023
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Ganapathy, S. (author), Meng, X. (author), Mossel, D.D.M. (author), Jagt, Mels (author), Brinks, D. (author)
Genetically encoded voltage indicators, particularly those based on microbial rhodopsins, are gaining traction in neuroscience as fluorescent sensors for imaging voltage dynamics with high-spatiotemporal precision. Here we establish a novel genetically encoded voltage indicator candidate based on the recently discovered subfamily of the...
journal article 2023
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Meng, X. (author), Ganapathy, S. (author), van Roemburg, Lars (author), Post, M.G. (author), Brinks, D. (author)
Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a...
review 2023
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Martinez Torres, C.E. (author), Grimbergen, Jos (author), Koopman, Jaap (author), Koenderink, G.H. (author)
Background: Fibrinogen is a plasma protein forming the fibrin scaffold of blood clots. Its mechanical properties therefore affect the risk of bleeding as well as thrombosis. There has been much recent interest in the biophysical mechanisms controlling fibrin mechanics; however, the role of molecular heterogeneity of the circulating fibrinogen...
journal article 2023
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Rafigh Doost, J. (author), Kolenov, D. (author), Pereira, S.F. (author)
It has been a widely growing interest in using silicon carbide (SiC) in high-power electronic devices. Yet, SiC wafers may contain killer defects that could reduce fabrication yield and make the device fall into unexpected failures. To prevent these failures from happening, it is very important to develop inspection tools that can detect,...
journal article 2023
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van der Werf, Verena M. (author), Zhao, J. (author), Koning, Jim S. (author), Nespoli, J. (author), Thieme, J. (author), Bus, M. (author), Savenije, T.J. (author)
Metal-halide perovskites deposited by wet-chemical deposition have demonstrated great potential for various electronic applications, including solar cells. A remaining question is how light-induced excess charges become distributed over such polycrystalline material. Here, we examine the local conductive properties of MAPbI<sub>3</sub> and...
journal article 2023
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Hebbar, Akshatha (author), Debraj, Devangshi (author), Acharya, Sriprasad (author), Puttapati, Sampath Kumar (author), Vatti, Anoop Kishore (author), Dey, P. (author)
Deep eutectic solvents (DESs) are industrially promising solvents and posses numerous applications in wide areas such as metal processing, synthesis media, synthesis of nanoparticles, gas sequestration and many more. In this work, we synthesized and investigated the performance of three deep eutectic solvents (DESs) i.e., reline, glyceline...
journal article 2023
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Maddalena, L. (author)
Optogenetics is a powerful addition to the spectrum of techniques available in neuroscience to investigate neurophysiology and unravel how neural circuit structure is related to circuit function. This technique relies on introducing lightsensitive proteins or molecules as actuators to transduce an optical signal into a physiological perturbation...
doctoral thesis 2022
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Lane, R. (author)
Multi-modal imaging techniques have become essential for better understanding fundamental questions in cell biology such as disease progression. While individual microscopy methods have rapidly advanced in recent years, the information content of any one imaging technique is limited to the type of contrast that particular technique is sensitive...
doctoral thesis 2022
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Chandrashekar, A. (author)
Most physical phenomena be it mechanical, chemical or biological are inherently nonlinear in nature. In fact, it is the linear phenomenon that is the exception rather than the rule. By harnessing these nonlinearities one can obtain far greater information about the underlying physics and develop more sensitive and efficient devices. This is...
doctoral thesis 2022
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Rejali, R. (author)
This thesis focuses on possible platforms for a bottom-up approach towards realizing and characterizing atomically assembled magnetic and electronic artificial lattices. For this, we make use of the scanning tunneling microscope (STM), which provides a local probe of the magnetic and electronic properties of the sample and allows for the atom-by...
doctoral thesis 2022
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Fu, Y. (author)
When high-temperature steels are loaded under under industrially relevant conditions not only creep (i.e. a time dependent strain increase even under nominally constant loading conditions) occurs, but also local damage is formed. At relatively short exposure times quasi-spherical micron-sized cavities form preferentially at the grain boundaries...
doctoral thesis 2022
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Marques Pereira, V. (author)
An approach to improve the performance of steels for fusion and fission reactors is to reinforce them with oxide nanoparticles. These can hinder dislocation and grain boundary movement and trap radiation-induced defects, thus increasing creep and radiation damage resistance. Steels containing these oxide particles are called ODS steels (Oxide...
doctoral thesis 2022
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Hung, S. (author), Llobet Rosell, Arnau (author), Jurriens, Daphne (author), Soloviev, O.A. (author), Kapitein, Lukas C. (author), Grußmayer, K.S. (author), Neukomm, Lukas J. (author), Verhaegen, M.H.G. (author), Smith, C.S. (author)
Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high...
journal article 2022
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Engelhardt, M.L.K. (author), Grußmayer, K.S. (author)
Optical microscopes allow us to study highly dynamic events from the molecular scale up to the whole animal level. However, conventional three-dimensional microscopy architectures face an inherent tradeoff between spatial resolution, imaging volume, light exposure and time required to record a single frame. Many biological processes, such as...
review 2022
Searched for: subject%3A%22microscopy%22
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