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Sathitruangsak, C. (author), Righolt, C.H. (author), Klewes, L. (author), Tammur, P. (author), Ilus, T. (author), Tamm, A. (author), Punab, M. (author), Olujohungbe, A. (author), Mai, S. (author)The mammalian nucleus has a distinct substructure that cannot be visualized directly by conventional microscopy. In this study, the organization of the DNA within the nucleus of multiple myeloma (MM) cells, their precursor cells (monoclonal gammopathy of undetermined significance; MGUS) and control lymphocytes of the representative patients is...journal article 2015
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Van Ommen, J.R. (author), Kooijman, D. (author), De Niet, M. (author), Talebi, M. (author), Goulas, A. (author)In this paper, the authors demonstrate a novel spatial atomic layer deposition (ALD) process based on pneumatic transport of nanoparticle agglomerates. Nanoclusters of platinum (Pt) of ?1?nm diameter are deposited onto titania (TiO2) P25 nanoparticles resulting to a continuous production of an active photocatalyst (0.12–0.31?wt. % of Pt) at a...journal article 2015
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Vdovin, Gleb (author), Gong, H. (author), Soloviev, O.A. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author)We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude in the pupil of the imaging system, without any reference beam,...journal article 2015
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Herfst, R. (author), Dekker, B. (author), Witvoet, G. (author), Crowcombe, W.E. (author), de Lange, D. (author), Sadeghian Marnani, H. (author)One of the major limitations in the speed of the atomic force microscope (AFM) is the bandwidth of the mechanical scanning stage, especially in the vertical (z) direction. According to the design principles of “light and stiff” and “static determinacy,” the bandwidth of the mechanical scanner is limited by the first eigenfrequency of the AFM...journal article 2015
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Antonello, J. (author)Microscopy is an essential tool for life sciences. Thanks to the development of confocal and multiphoton microscopy, scientists are able to obtain high-resolution 3D views of biological specimens. Nevertheless, spatial variations in the index of refraction within specimens cause aberrations that degrade the quality of the 3D views. One can...doctoral thesis 2014
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Liv, N. (author)A combined use of fluorescence and light microscopy is a powerful approach to further increase our understanding in biological systems of structure-function relations at cellular and sub-cellular levels. The power of fluorescence microscopy (FM) is to spectrally resolve and visualize individual proteins with endogenous or immuno- fluorescent...doctoral thesis 2014
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Narváez, A.C. (author)Cathodoluminescence (CL), the excitation of light by an electron beam, has gained attention as an analysis tool for investigating the optical response of a structure, at a resolution that approaches that in electron microscopy, in the nanometer range. However, the application possibilities are limited because the use of transparent substrates,...doctoral thesis 2014
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Passeri, D. (author), Dong, C. (author), Reggente, M. (author), Angeloni, L. (author), Barteri, M. (author), Scaramuzzo, F.A. (author), De Angelis, F. (author), Marinelli, F. (author), Antonelli, F. (author), Rinaldi, F. (author), Marianecci, C. (author), Carafa, M. (author), Sorbo, A. (author), Sordi, D. (author), Arends, I.W.C.E. (author), Rossi, M. (author)Magnetic force microscopy (MFM) is an atomic force microscopy (AFM) based technique in which an AFM tip with a magnetic coating is used to probe local magnetic fields with the typical AFM spatial resolution, thus allowing one to acquire images reflecting the local magnetic properties of the samples at the nanoscale. Being a well established tool...journal article 2014
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Verduin, T. (author), Kruit, P. (author), Hagen, C.W. (author)We investigated the off-line metrology for line edge roughness (LER) determination by using the discrete power spectral density (PSD). The study specifically addresses low-dose scanning electron microscopy (SEM) images in order to reduce the acquisition time and the risk of resist shrinkage. The first attempts are based on optimized elliptic...journal article 2014
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Burri, S. (author), Maruyama, Y. (author), Michalet, X. (author), Regazzoni, F. (author), Bruschini, C. (author), Charbon, E. (author)We present the architecture and three applications of the largest resolution image sensor based on single-photon avalanche diodes (SPADs) published to date. The sensor, fabricated in a high-voltage CMOS process, has a resolution of 512 × 128 pixels and a pitch of 24 ?m. The fill-factor of 5% can be increased to 30% with the use of microlenses....journal article 2014
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Tirumalasetty, G.K. (author), Fang, C.M. (author), Jansen, J. (author), Yokosawa, T. (author), Boeije, M.J.F. (author), Sietsma, J. (author), Van Huis, M.A. (author), Zandbergen, H.W. (author)Chromium (Cr), Manganese (Mn), and Carbon (C) are well known alloying elements used in technologically important alloy steels and advanced high strength steels. It is known that binary CrCx and MnCx carbides can be formed in steels, but in this study we reveal for the first time that Cr and Mn were found combined in novel ternary cementite type ...journal article 2014
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Sadeghian Marnani, H. (author), Herfst, R.W. (author), Van den Dool, T.C. (author), Crowcombe, W.E. (author), Winters, J. (author), Kramer, G.F.I.J. (author)Scanning probe microscopy (SPM) is a promising candidate for accurate assessment of metrology and defects on wafers and masks, however it has traditionally been too slow for high-throughput applications, although recent developments have significantly pushed the speed of SPM [1,2]. In this paper we present new results obtained with our...conference paper 2014
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Righolt, C.H. (author), Guffei, A. (author), Knecht, H. (author), Young, I.T. (author), Stallinga, S. (author), Van Vliet, L.J. (author), Mai, S. (author)Advances in light microscopy have enabled the visualization of DNA in the interphase nucleus with more detail than is visible with conventional light microscopy. The nuclear architecture is assumed to be different in cancer cells compared to normal cells. In this paper we have studied, for the first time, the organization of nuclear DNA and that...journal article 2014
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Antonello, J. (author), Van Werkhoven, T. (author), Verhaegen, M. (author), Truong, H.H. (author), Keller, C.U. (author), Gerritsen, H.C. (author)Optical aberrations have detrimental effects in multiphoton microscopy. These effects can be curtailed by implementing model-based wavefront sensorless adaptive optics, which only requires the addition of a wavefront shaping device, such as a deformable mirror (DM) to an existing microscope. The aberration correction is achieved by maximizing a...journal article 2014
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Broeken, J. (author), Rieger, B. (author), Stallinga, S. (author)We propose a method for simultaneously measuring the position and emission color of single fluorescent emitters based on the use of a large pitch diffraction grating in the emission light path. The grating produces satellite spots adjacent to the main spot; the relative distance between the spots is a measure for the emission wavelength. We...journal article 2014
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Roy, S. (author), Assafrao, A.C. (author), Pereira, S.F. (author), Urbach, H.P. (author)Inspection tools for nano-particle contamination on a planar substrate surface is a critical problem in micro-electronics. The present solutions are either expensive and slow or inexpensive and fast but have low sensitivity because of limitations due to diffraction. Most of them are also substrate specific. In this article we report how Coherent...journal article 2014
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Yalcin, A.O. (author), Fan, Z. (author), Goris, B. (author), Li, W.F. (author), Koster, R.S. (author), Fang, C.M. (author), Van Blaaderen, A. (author), Casavola, M. (author), Tichelaar, F.D. (author), Bals, S. (author), Van Tendeloo, G. (author), Vlugt, T.J.H. (author), Vanmaekelbergh, D. (author), Zandbergen, H.W. (author), Van Huis, M.A. (author)Here, we show a novel solid?solid?vapor (SSV) growth mechanism whereby epitaxial growth of heterogeneous semiconductor nanowires takes place by evaporation-induced cation exchange. During heating of PbSe-CdSe nanodumbbells inside a transmission electron microscope (TEM), we observed that PbSe nanocrystals grew epitaxially at the expense of CdSe...journal article 2014
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Damsgaard, C.D. (author), Zandbergen, H.W. (author), Hansen, T.W. (author), Chorkendorff, I. (author), Wagner, J.B. (author)Specimen transfer under controlled environment conditions, such as temperature, pressure, and gas composition, is necessary to conduct successive complementary in situ characterization of materials sensitive to ambient conditions. The in situ transfer concept is introduced by linking an environmental transmission electron microscope to an in...journal article 2014
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Righolt, C.H. (author), Mai, S. (author), Van Vliet, L.J. (author), Stallinga, S. (author)The quality of the reconstructed image in structured illumination microscopy (SIM) depends on various aspects of the image filtering process. To optimize the trade-off between resolution and ringing artifacts, which lead to negative intensities, we extend Lukosz-bound filtering to 3D SIM and derive the parametrization of the 3D SIM cut-off. We...journal article 2014
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Van Werkhoven, T.I.M. (author), Antonello, J. (author), Truong, H.H. (author), Verhaegen, M. (author), Gerritsen, H.C. (author), Keller, C.U. (author)Deep imaging in turbid media such as biological tissue is challenging due to scattering and optical aberrations. Adaptive optics has the potential to compensate the tissue aberrations. We present a wavefront sensing scheme for multi-photon scanning microscopes using the pulsed, near-infrared light reflected back from the sample utilising...journal article 2014