Searched for: subject%3A%22penicillin%22
(1 - 12 of 12)
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Jánoska, A. (author), Buijs, Joran (author), van Gulik, W.M. (author)
In large scale fermentors the cultivated cells are exposed to dynamic changes in the nutrient concentrations due to imperfect mixing. Based on the characterization of these nutrient gradients in space and time, a rational scale down design can be obtained. This study focuses on the combined gradients of dissolved sugar and oxygen...
journal article 2023
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Wei, P. (author), Haringa, C. (author), Portela, L. (author), Noorman, H.J. (author)
This study focuses on the metabolic impacts of simultaneous glucose and oxygen concentration gradients on penicillin production in an industrial-scale fermentor, using the computational fluid dynamics-cellular reaction dynamics approach. Inclusion of oxygen-coupling considerably impacts the glucose uptake and resulting penicillin productivity...
journal article 2023
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Wieringa, André (author), Ewoldt, Tim (author), Gangapersad, Ravish N. (author), Gijsen, Matthias (author), Parolya, N. (author), Kats, Chantal J. A. R. (author), Spriet, Isabel (author), Endeman, Henrik (author), Haringman, Jasper J. (author)
In the intensive care unit (ICU), infection-related mortality is high. Although adequate antibiotic treatment is essential in infections, beta-lactam target non-attainment occurs in up to 45% of ICU patients, which is associated with a lower likelihood of clinical success. To optimize antibiotic treatment, we aimed to develop beta-lactam target...
journal article 2023
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Jánoska, A. (author), Verheijen, Jelle J. (author), Tang, W. (author), Lee, Queenie (author), Sikkema, Baukje (author), van Gulik, W.M. (author)
In large-scale bioreactors, there is often insufficient mixing and as a consequence, cells experience uneven substrate and oxygen levels that influence product formation. In this study, the influence of dissolved oxygen (DO) gradients on the primary and secondary metabolism of a high producing industrial strain of Penicillium chrysogenum was...
journal article 2022
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Zhang, Zheming (author), Wang, Fang (author), Pang, Y. (author), Yan, Gaowei (author)
The problem of misalignment of the original measurement model is caused by nonlinear, time-varying characteristic of the batch process. In this paper, a method based on geodesic flow kernel (GFK) for feature transfer is proposed. By mapping data into the manifold space, the feature transfer from source domain to target domain is implemented....
conference paper 2020
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Sand, M. (author)
Penicillin is a serendipitous discovery par excellence. But, what does this say about Alexander Fleming’s praiseworthiness? Clearly, Fleming would not have received the Nobel Prize, had not a mould accidently entered his laboratory. This seems paradoxical, since it was beyond his control. The present article will first discuss Fleming’s...
journal article 2019
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Deshmukh, A.T. (author)
Even 80 years after the discovery of penicillin, it still holds 16% of total antibiotics market. This makes it crucial, from an economical point of view, to improve our understanding of the production organism Penicillium chrysogenum to maximize the penicillin production, as its theoretical yields are far from reached. With the advancement in...
doctoral thesis 2013
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Douma, R.D. (author)
Penicillin has been produced on an industrial scale for several decades. The improvements in its production process, in terms of product yields and production rates, present an unprecedented success in fermentation technology. However, the obtained product yields still remain far from their theoretical maximum. More insight in the regulation of...
doctoral thesis 2010
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Harris, D.M. (author)
With an over 1000-fold improvement in specific productivity since its discovery, penicillin is one of the very successful examples of industrial biotechnology. Although classical strain improvement programmes have been a major contributor to this success, the wish for a more rational approach towards improvements has driven the work described in...
doctoral thesis 2008
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Kleijn, R.J. (author)
The 13C-labeling technique is a powerful characterization tool within the field of metabolic engineering aimed at determining intracellular steady state fluxes. The provided metabolic snapshots enable researchers to better understand and predict the phenotypic behavior of a micro-organism as a result of genetic alterations and/or different...
doctoral thesis 2007
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Nasution, U.D.M. (author)
Abstract not available
doctoral thesis 2007
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Janssen, M.H.A. (author)
The beta-lactam antibiotics are the most important class of antibiotics used today. In the last decade the production routes of these antibiotics have shifted from chemical routes to more environmentally benign routes using the enzyme penicillin G acylase. For both practical and economical reasons the enzyme is immobilised. As the kinetically...
doctoral thesis 2006
Searched for: subject%3A%22penicillin%22
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