Searched for: subject:"proteins"
(1 - 20 of 99)

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Restrepo Perez, L. (author)
The function and phenotype of a cell is determined by a complex network of interactions between DNA, RNA, proteins and metabolites. Therefore, a comprehensive approach that integrates genomics, transcriptomics, proteomics, and metabolomics is necessary to achieve full understanding of biological processes and disease. Recent technological...
doctoral thesis 2019
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Kok, M.W.A. (author)
The microtubule cytoskeleton is an intracellular polymer network involved in cell shape, cell motility, and cell division. Microtubules are self-assembling, dynamic polymers composed of tubulin proteins that alternate between phases of growth and shrinkage, a behaviour known as “dynamic instability”. A key feature of microtubules is their...
doctoral thesis 2019
document
Subramanian, Kalyanasundaram (author), Mitusińska, Karolina (author), Raedts, John (author), Almourfi, Feras (author), Joosten, Henk Jan (author), Hendriks, Sjon (author), Sedelnikova, Svetlana E. (author), Kengen, Servé W.M. (author), Hagen, W.R. (author), More Authors (author)
The cupin-type phosphoglucose isomerase (PfPGI) from the hyperthermophilic archaeon Pyrococcus furiosus catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate. We investigated PfPGI using protein-engineering bioinformatics tools to select functionally-important residues based on correlated mutation analyses. A...
journal article 2019
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Tan Timur, Ufuk (author), Caron, Marjolein (author), van den Akker, Guus (author), van der Windt, Anna (author), Visser, Jenny A. (author), van Rhijn, Lodewijk (author), Weinans, H.H. (author), Welting, Tim (author), Emans, Pieter (author), Jahr, H. (author)
During standard expansion culture (i.e., plasma osmolarity, 280 mOsm) human articular chondrocytes dedifferentiate, making them inappropriate for autologous chondrocyte implantation to treat cartilage defects. Increasing the osmolarity of culture media to physiological osmolarity levels of cartilage (i.e., 380 mOsm), increases collagen type...
journal article 2019
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Kozijn, A. E. (author), Tartjiono, M. T. (author), Ravipati, S. (author), van der Ham, F. (author), Barrett, D. A. (author), Mastbergen, S.C. (author), Korthagen, N. M. (author), Lafeber, F. P.J.G. (author), Zuurmond, A. M. (author), Bobeldijk, I. (author), Weinans, H.H. (author), Stoop, R (author)
Objective: C-reactive protein (CRP) levels can be elevated in osteoarthritis (OA) patients. In addition to indicating systemic inflammation, it is suggested that CRP itself can play a role in OA development. Obesity and metabolic syndrome are important risk factors for OA and also induce elevated CRP levels. Here we evaluated in a human CRP ...
journal article 2019
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Zhao, Shidi (author), Restrepo Perez, L. (author), Soskine, Misha (author), Maglia, Giovanni (author), Joo, C. (author), Dekker, C. (author), Aksimentiev, A. (author)
Ion channels form the basis of information processing in living cells by facilitating the exchange of electrical signals across and along cellular membranes. Applying the same principles to man-made systems requires the development of synthetic ion channels that can alter their conductance in response to a variety of external manipulations....
journal article 2019
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Consorti Bussamra, B. (author), Castro Gomes, Joana (author), Freitas, Sindelia (author), Mussatto, Solange I. (author), da Costa, Aline Carvalho (author), van der Wielen, L.A.M. (author), Ottens, M. (author)
Aqueous two-phase systems (ATPS) can be applied to enzymatic reactions that are affected by product inhibition. In the biorefinery context, sugars inhibit the cellulolytic enzymes in charge of converting the biomass. Here, we present a strategy to select an ATPS (formed by polymer and salt) that can separate sugar and enzymes. This automated...
journal article 2019
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Vendel, K.J.A. (author), Tschirpke, S. (author), Shamsi, F. (author), Dogterom, A.M. (author), Laan, L. (author)
Cell polarity - the morphological and functional differentiation of cellular compartments in a directional manner - is required for processes such as orientation of cell division, directed cellular growth and motility. How the interplay of components within the complexity of a cell leads to cell polarity is still heavily debated. In this...
review 2019
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Idema, T. (author), Kraft, Daniela J. (author)
Protein inclusions in the membranes of living cells interact via the deformations they impose on that membrane. Such membrane-mediated interactions lead to sorting and self-assembly of the inclusions, as well as to membrane remodelling, crucial for many biological processes. For the past decades, theory, numerical calculations and experiments...
review 2019
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Dias Ribeiro de Carvalho, V.I. (author)
Proteins are one of the most important constituents of cells, since they are responsible for various processes such as replication, translation, etc. Interestingly, proteins are also responsible for the degradation of proteins that do not assemble well, and/or which are not necessary anymore inside the cell. This process of proteins destruction...
doctoral thesis 2018
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Scholtissek, Anika (author), Gädke, Eric (author), Paul, C.E. (author), Westphal, Adrie H. (author), van Berkel, Willem J.H. (author), Tischler, Dirk (author)
Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from Rhodococcus opacus 1CP (WT) as well as its engineered variants C25A, C25S and C25G. OYERo2a in its redox resting state ...
journal article 2018
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Verschueren, D.V. (author)
Grabbing a single molecule and inspecting its contents is far from easy. Apart from the small size of the objects, biomolecules shake, shimmer, and bounce around a tremendous amount. How can one gently control something that small (without squashing or destroying it) and still be able to tell what it is? The work in this thesis is exactly aimed...
doctoral thesis 2018
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Marinus, tycho (author)
While the sequencing era has greatly increased the amount of protein sequences, in the eld of protein structure this increase has not occurred, mostly due to the high cost and workload of protein structure determination. This has resulted in a large gap between the amount of protein sequences and protein structures. In the case of TB drug...
master thesis 2018
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Pronk, J.W. (author)
doctoral thesis 2018
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Poltorak, L. (author), Oonk, S. (author), Sudholter, Ernst J. R. (author), de Puit, M. (author)
The behaviour of acid phosphatase at an electrified liquid–liquid interface was studied in this work. It was found that only the protonated form of the protein can undergo interfacial adsorption which is affected by the pH of the aqueous phase. With ion transfer voltammetry we could detect acid phosphatase in concentrations as low as 0.1 μM....
journal article 2018
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Osipov, Eugeny M. (author), Lilina, Anastasia V. (author), Tsallagov, Stanislav I. (author), Safonova, Tatyana N. (author), Sorokin, Dimitry Y. (author), Tikhonova, Tamara V. (author), Popova, Vladimir O. (author)
complex was determined by X-ray diffraction...
journal article 2018
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Ananth, A.N. (author)
Nuclear pore complexes acts as a gatekeeper for molecular transport between the nucleus and the cytoplasm in eukaryotic cells. The central NPC channel is filled with intrinsically disordered FG domains (phenylalanine (F), glycine (G)) that are responsible for the fascinating selectivity of NPCs, for which the underlying mechanism is still under...
doctoral thesis 2018
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Giordano, G. (author)
This paper considers two models of ceramide-transfer protein (CERT)-mediated ceramide transfer at the trans-Golgi network proposed in the literature, short distance shuttle and neck swinging, and seeks structural (parameter-free) features of the two models, which rely exclusively on the peculiar interaction network and not on specific parameter...
journal article 2018
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Diepeveen, E.T. (author), Gehrmann, T. (author), Abeel, T.E.P.M.F. (author), Pourquié, Valérie (author), Laan, L. (author)
The combined actions of proteins in networks underlie all fundamental cellular functions. Deeper insights into the dynamics of network composition across species and their functional consequences are crucial to fully understand protein network evolution. Large-scale comparative studieswith high phylogenetic resolution arenowfeasible through...
journal article 2018
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Rho, Hoon Suk (author), Hanke, A.T. (author), Ottens, M. (author), Gardeniers, Han J.G.E. (author)
A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro-column, pH gradient generation, and fraction collection in a single device. Using a sieve micro-valve, anion exchange particles were packed into a microchannel in order to realize a solid-phase absorption column. To fractionate proteins...
journal article 2018
Searched for: subject:"proteins"
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