L
L Wu
14 records found
1
Authored
MIRACLE
Mass Isotopomer Ratio Analysis of U-13C-Labeled Extracts. A New Method for Accurate Quantification of Changes in Concentrations of Intracellular Metabolites
First, we report the application of stable isotope dilution theory in metabolome characterization of aerobic glucose limited chemostat culture of S. cerevisiae CEN.PK 113-7D using liquid chromatography - electrospray ionization MS/MS (LC-ESI-MS/MS). A glucose-limited chemostat ...
In vivo kinetics of Saccharomyces cerevisiae are studied, in a time window of 150 s, by analyzing the response of O2, and CO2, in the fermentor off-gas after perturbation of chemostat cultures by metabolite pulses. Here, a new mathematical method is presented for the estimation o
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