Truck platooning refers to the automated operation of multiple trucks. High expectations rest on truck platooning as to alleviate negative impacts caused by road freight transport like congestion, accidents and pollution. Furthermore, driver shortages may be partially solved by the concept of truck platooning. This study identified enablers and barriers of truck platooning and assessed their effect on the potential implementation of the concept. The implementation and possible adoption of truck platooning as a transport mode is a complex and uncertain process. The magnitude of the consequences of platooning are in general unknown and many different stakeholders should be involved in the implementation process. Based on this research it can be concluded that several barriers still have to be mitigated and that cooperation between different competing companies and industries is essential for the successful implementation of truck platooning.