In this paper, a new moisture conditioning protocol which attempts to distinguish the contributions of long- and short-term moisture damage, i.e. moisture diffusion and cyclic pore pressure generation, in asphalt mixtures is presented. The capability of the proposed protocol to rank various asphalt mixtures of known field performance for their short- and long-term sensitivity to moisture is evaluated on the basis of the Tensile Strength Ratio. Asphalt specimens with different types of aggregates and asphalt binders were conditioned by various combinations of water bath immersion and cyclic pore pressures by means of the Moisture Induced Sensitivity Tester. The results show that the proposed conditioning protocol can be used to evaluate the moisture susceptibility of asphalt mixtures and distinguish among mixtures with different moisture damage characteristics. In addition, it is shown that the use of cyclic pore pressures has a significant effect and can be used as an accelerated moisture conditioning procedure.