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Acute Central Neuropeptide Y Administration Increases Food Intake but Does Not Affect Hepatic Very Low-Density Lipoprotein (Vldl) Production in Mice
Objective: Central neuropeptide Y (NPY) administration stimulates food intake in rodents. In addition, acute modulation of central NPY signaling increases hepatic production of very low-density lipoprotein (VLDL)-triglyceride (TG) in rats. As hypertriglyceridemia is an important risk factor for atherosclerosis, for which well-established mouse models are available, we set out to validate the effect of NPY on hepatic VLDL-TG production in mice, to ultimately investigate whether NPY, by increasing VLDL production, contributes to the development of atherosclerosis. Research Design and Methods: Male C57Bl/6J mice received an intracerebroventricular (i.c.v.) cannula into the lateral (LV) or third (3V) ventricle of the brain. One week later, after a 4 h fast, the animals received an intravenous (i.v.) injection of Tran35S (100 μCi) followed by tyloxapol (500 mg/kg body weight; BW), enabling the study of hepatic VLDL-apoB and VLDL-TG production, respectively. Immediately after the i.v. injection of tyloxapol, the animals received either an i.c.v. injection of NPY (0.2 mg/kg BW in artificial cerebrospinal fluid; aCSF), synthetic Y1 receptor antagonist GR231118 (0.5 mg/kg BW in aCSF) or vehicle (aCSF), or an i.v. injection of PYY3-36 (0.5 mg/kg BW in PBS) or vehicle (PBS). Results: Administration of NPY into both the LV and 3V increased food intake within one hour after injection (+164%, p<0.001 and +367%, p<0.001, respectively). NPY administration neither in the LV nor in the 3V affected hepatic VLDL-TG or VLDL-apoB production. Likewise, antagonizing central NPY signaling by either PYY3-36 or GR231118 administration did not affect hepatic VLDL production. Conclusion: In mice, as opposed to rats, acute central administration of NPY increases food intake without affecting hepatic VLDL production. These results are of great significance when extrapolating findings on the central regulation of hepatic VLDL production between species. © 2013 Geerling et al.
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[Abstract]
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2 |
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Hepatic Steatosis: A Mediator of the Metabolic Syndrome. Lessons from Animal Models
article |
2004
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Author: |
Boer, M. den
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Voshol, P.J.
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Kuipers, F.
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Havekes, L.M.
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Romijn, J.A.
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Keywords: |
Biology · Biomedical Research · Fatty acid metabolism · Glucose metabolism · Insulin resistance · Lipoprotein metabolism · Mouse models · Alcohol · Triacylglycerol · Animal experiment · Animal model · Disease association · Dyslipidemia · Endocrine system · Genetic transcription · Glucose metabolism · Lnsulin resistance · Lipid liver level · Metabolic syndrome X · Mouse · Non insulin dependent diabetes mellitus · Nonhuman · Partition coefficient · Pathophysiology · Rodent · Short survey · Adipose Tissue · Animals · Diabetes Mellitus, Type 2 · Dogs · Fatty Acids · Fatty Liver · Glucose · Homeostasis · Humans · Hyperlipidemias · Insulin Resistance · Liver · Metabolic Syndrome X · Mice · Models, Animal · Models, Biological · Obesity · Rats · Rats, Zucker · Transcription, Genetic · Triglycerides
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Epidemiological studies in humans, as well as experimental studies in animal models, have shown an association between visceral obesity and dyslipidemia, insulin resistance, and type 2 diabetes mellitus. Recently, attention has been focused on the excessive accumulation of triglycerides (TG) in the liver as part of this syndrome. In this review, important principles of the pathophysiological involvement of the liver in the metabolic syndrome obtained in rodent models are summarized. We focus on non-alcoholic causes of steatosis, because the animal experiments we refer to did not include alcohol as an experimental condition. In general, there is continuous cycling and redistribution of non-oxidized fatty acids between different organs. The amount of TG in an intrinsically normal liver is not fixed but can readily be increased by nutritional, metabolic, and endocrine interactions involving TG/free fatty acid (FFA) partitioning and TG/FFA metabolism. Several lines of evidence indicate that hepatic TG accumulation is also a causative factor involved in hepatic insulin resistance. Complex interactions between endocrine, metabolic, and transcriptional pathways are involved in TG-induced hepatic insulin resistance. Therefore, the liver participates passively and actively in the metabolic derangements of the metabolic syndrome. We speculate that similar mechanisms may also be involved in human pathophysiology. Chemicals / CAS: alcohol, 64-17-5; Fatty Acids; Glucose, 50-99-7; Triglycerides
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[Abstract]
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3 |
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Hepatic glucose production is more sensitive to insulin-mediated inhibition than hepatic VLDL-triglyceride production
Insulin is an important inhibitor of both hepatic glucose output and hepatic VLDL-triglyceride (VLDL-TG) production. We investigated whether both processes are equally sensitive to insulin-mediated inhibition. To test this, we used euglycemic clamp studies with four increasing plasma concentrations of insulin in wild-type C57Bl/6 mice. By extrapolation, we estimated that half-maximal inhibition of hepatic glucose output and hepatic VLDL-TG production by insulin were obtained at plasma insulin levels of ∼3.6 and ∼6.8 ng/ml, respectively. In the same experiments, we measured that half-maximal decrease of plasma free fatty acid levels and half-maximal stimulation of peripheral glucose uptake were reached at plasma insulin levels of ∼3.0 and ∼6.0 ng/ml, respectively. We conclude that, compared with insulin sensitivity of hepatic glucose output, peripheral glucose uptake and hepatic VLDL-TG production are less sensitive to insulin. Copyright © 2006 the American Physiological Society. Chemicals / CAS: insulin, 9004-10-8; Blood Glucose; Fatty Acids, Nonesterified; Glucose, 50-99-7; Hypoglycemic Agents; Insulin, 11061-68-0; Lipoproteins, VLDL; Triglycerides
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[Abstract]
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4 |
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Effect of plasma triglyceride metabolism on lipid storage in adipose tissue: Studies using genetically engineered mouse models
The obesity epidemic is associated with an increased incidence of type 2 diabetes, cardiovascular morbidity and various types of cancer. A better insight into the molecular mechanisms that underlie adipogenesis and obesity may result in novel therapeutic handles to fight obesity and these associated diseases. Adipogenesis is determined by the balance between uptake of fatty acids (FA) from plasma into adipocytes, intracellular FA oxidation versus esterification of FA into triglycerides (TG), lipolysis of TG by intracellular lipases, and secretion of FA from adipocytes. Here, we review the mechanisms that are specifically involved in the entry of FA into adipose tissue. In plasma, these originating FA are either present as TG within apoB-containing lipoproteins (i.e. chylomicrons and VLDL) or as free FA bound to albumin. Kinetic studies, however, have revealed that TG are the major source of FA entering adipose tissue, both in the fed and fasted condition. In fact, studies with genetically engineered mice have revealed that the activity of lipoprotein lipase (LPL) is a major determinant for the development of obesity. As a general rule, high fat diet-induced adipogenesis is aggravated by stimulated LPL activity (e.g. by adipose tissue-specific overexpression of LPL or deficiency for apoCIII), and attenuated by inhibited LPL activity (e.g. by adipose-specific deficiency for LPL, overexpression of apoCI or angptl4, or by deficiency for apoE or the VLDL receptor). In addition, we describe that the trans-membrane transport of FA and cytoplasmic binding of FA in adipocytes can also dramatically affect adipogenesis. The relevance of these findings for human pathophysiology is discussed. © 2009 Elsevier B.V. All rights reserved.
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[Abstract]
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5 |
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Apolipoprotein CI enhances the biological response to LPS via the CD14/TLR4 pathway by LPS-binding elements in both its N- and C-terminal helix
Timely sensing of lipopolysaccharide (LPS) is critical for the host to fight invading Gram-negative bacteria. We recently showed that apolipoprotein CI (apoCI) (apoCI<sub>1-57</sub>) avidly binds to LPS, involving an LPS-binding motif (apoCI<sub>48-54</sub>), and thereby enhances the LPS-induced inflammatory response. Our current aim was to further elucidate the structure and function relationship of apoCI with respect to its LPS-modulating characteristics and to unravel the mechanism by which apoCI enhances the biological activity of LPS. We designed and generated N- and C-terminal apoCI-derived peptides containing varying numbers of alternating cationic/hydrophobic motifs. ApoCI <sub>1-38</sub>, apoCI<sub>1-30</sub>, and apoCI<sub>35-57</sub> were able to bind LPS, whereas apoCI<sub>1-23</sub> and apoCI<sub>46-57</sub> did not bind LPS. In line with their LPS-binding characteristics, apoCI<sub>1-38</sub>, apoCI<sub>1-30</sub>, and apoCI<sub>35-57</sub> prolonged the serum residence of <sup>125</sup>I-LPS by reducing its association with the liver. Accordingly, both apoCI<sub>1-30</sub> and apoCI<sub>35-57</sub> enhanced the LPS-induced TNFα response in vitro (RAW 264.7 macrophages) and in vivo (C57Bl/6 mice). Additional in vitro studies showed that the stimulating effect of apoCI on the LPS response resembles that of LPSbinding protein (LBP) and depends on CD14/ Toll-like receptor 4 signaling.<sup>jlr</sup> We conclude that apoCI contains structural elements in both its N-terminal and C-terminal helix to bind LPS and to enhance the proinflammatory response toward LPS via a mechanism similar to LBP. Copyright © 2010 by the American Society for Biochemistry and Molecular Biology, Inc.
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[PDF]
[Abstract]
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6 |
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Leptin deficiency per se dictates body composition and insulin action in ob/ob mice
article |
2008
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Author: |
Hoek, A.M. van den
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Teusink, B.
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Voshol, P.J.
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Havekes, L.M.
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Romijn, J.A.
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Pijl, H.
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Keywords: |
Biology · Adiposity hormone · Brain · Insulin resistance · Metabolism · Type 2 diabetes mellitus · glucose · insulin · leptin · animal experiment · animal tissue · article · body composition · body weight · caloric restriction · controlled study · diet restriction · female · gluconeogenesis · glucose clamp technique · glucose metabolism · hormone deficiency · insulin resistance · insulin sensitivity · lean body weight · leptin deficiency · male · mouse · mouse strain · nonhuman · obesity · pathophysiology · priority journal · quantitative analysis · signal transduction · wild type · Animals · Blood Glucose · Body Composition · Body Weight · Fatty Acids, Nonesterified · Female · Food Deprivation · Glucose · Glucose Clamp Technique · Growth and Development · Insulin · Leptin · Male · Mice · Mice, Inbred C57BL · Mice, Obese · Mice, Transgenic · Obesity
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Obese humans are often insulin- and leptin resistant. Since leptin can affect glucose metabolism, it is conceivable that a lack of leptin signal transduction contributes to insulin resistance. It remains unclear whether leptin affects glucose metabolism via peripheral and/or central mechanistic routes. In the present study, we aimed: (i) to determine the relative contributions of lack of leptin signal transduction and adiposity to insulin resistance and (ii) to establish the impact of central leptin action on glucose metabolism. To address the first point, ob/ob mice were subjected to severe calorie restriction, so that their body weight became similar to that of wild-type mice. Insulin sensitivity was measured in obese ob/ob, lean (food restricted) ob/ob and lean, weight-matched wild-type mice. To address the second point, leptin (or vehicle) was i.c.v. infused to the lateral cerebral ventricle of ob/ob mice and insulin sensitivity was determined. Hyperinsulinaemic euglyceamic clamps were used to quantify insulin sensitivity. Food restriction barely affected body composition, although it profoundly curtailed body weight. Insulin suppressed hepatic glucose production (HGP) to a greater extent in lean ob/ob than in obese ob/ob mice, but its impact remained considerably less than in wild-type mice (% suppression: 11.8 ± 8.9 versus 1.3 ± 1.1 versus 56.6 ± 13.0%/nmol, for lean, obese ob/ob and wild-type mice, respectively; P < 0.05). The insulin-mediated glucose disposal (GD) of lean ob/ob mice was also in between that of obese ob/ob and wild-type mice (37.5 ± 21.4 versus 25.1 ± 14.6 versus 59.6 ± 17.3 μmol/min/kg/nmol of insulin, respectively; P < 0.05 wild-type versus obese ob/ob mice). Leptin infusion acutely enhanced both hepatic insulin sensitivity (insulin-induced inhibition of HGP) and insulin-mediated GD (9.1 ± 2.4 versus 5.0± 2.7%/nmol of insulin, and 25.6 ± 5.6 versus 13.6 ± 4.8 μmol/min/kg/ nmol of insulin, respectively; P < 0.05 for both comparisons) in ob/ob mice. Both a lack of leptin signals and adiposity may contribute to insulin resistance in obese individuals. Diminution of central leptin signalling can critically affect glucose metabolism in these individuals. © 2008 The Authors. Journal Compilation © 2008 Blackwell Publishing Ltd.
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[Abstract]
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7 |
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Acute inhibition of hepatic β-oxidation in APOE*3Leiden mice does not affect hepatic VLDL secretion or insulin sensitivity
article |
2005
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Author: |
Duivenvoorden, I.
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Teusink, B.
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Rensen, P.C.N.
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Kuipers, F.
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Romijn, J.A.
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Havekes, L.M.
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Voshol, P.J.
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Keywords: |
Biology · Biomedical Research · Fatty acids · Glucose metabolism · Steatosis · Triglycerides · Very low density lipoprotein · 3 hydroxybutyrate dehydrogenase · carnitine palmitoyltransferase inhibitor · cholesterol · insulin · palmoxiric acid · triacylglycerol · very low density lipoprotein · apolipoprotein E · apolipoprotein E3 · apolipoprotein E3 (Leidein) · fatty acid · messenger RNA · analytic method · animal cell · animal experiment · article · cholesterol blood level · comparative study · controlled study · fatty acid oxidation · gluconeogenesis · insulin blood level · insulin sensitivity · ketogenesis · mouse · nonhuman · transgenic mouse · animal · blood · genetics · insulin resistance · metabolism · oxidation reduction reaction · physiology · secretion · Animals · Apolipoprotein E3 · Apolipoproteins E · Fatty Acids, Nonesterified · Insulin Resistance · Lipoproteins, VLDL · Liver · Male · Mice · Mice, Transgenic · Oxidation-Reduction · RNA, Messenger
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Hepatic VLDL and glucose production is enhanced in type 2 diabetes and associated with hepatic steatosis. Whether the derangements in hepatic metabolism are attributable to steatosis or to the increased availability of FA metabolites is not known. We used methyl palmoxirate (MP), an inhibitor of carnitine palmitoyl transferase I, to acutely inhibit hepatic FA oxidation and investigated whether the FAs were rerouted into VLDL secretion and whether this would affect hepatic glucose production. After an overnight fast, male APOE3*Leiden transgenic mice received an oral dose of 10 mg/kg MP. Administration of MP led to an 83% reduction in plasma β-hydroxybutyrate (ketone body) levels compared with vehicle-treated mice (0.47 ± 0.07 vs. 2.81 ± 0.16 mmol/l, respectively; P < 0.01), indicative of impaired ketogenesis. Plasma FFA levels were increased by 32% and cholesterol and insulin levels were decreased by 17% and 50%, respectively, in MP-treated mice compared with controls. MP treatment led to a 30% increase in liver triglyceride (TG) content. Surprisingly, no effect on hepatic VLDL-TG production was observed between the groups at 8 h after MP administration. In addition, the capacity of insulin to suppress endogenous glucose production was unaffected in MP-treated mice compared with controls. In conclusion, acute inhibition of FA oxidation increases hepatic lipid content but does not stimulate hepatic VLDL secretion or reduce insulin sensitivity. Copyright © 2005 by the American Society for Biochemistry and Molecular Biology, Inc. Chemicals / CAS: 3 hydroxybutyrate dehydrogenase, 9028-38-0; cholesterol, 57-88-5; insulin, 9004-10-8; palmoxiric acid, 68170-97-8, 81556-15-2; apolipoprotein E3 (Leidein); Apolipoprotein E3; Apolipoproteins E; Fatty Acids, Nonesterified; Lipoproteins, VLDL; RNA, Messenger
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[PDF]
[Abstract]
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8 |
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Inhibition of the central melanocortin system decreases brown adipose tissue activity
article |
2014
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Author: |
Kooijman, S.
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Boon, M.R.
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Parlevliet, E.T.
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Geerling, J.J.
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Pol, V. van de
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Romijn, J.A.
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Havekes, L.M.
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Meurs, I.
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Rensen, P.C.N.
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Keywords: |
Biology · Energy expenditure · Liver · Triglycerides · Very low density lipoprotein · White adipose tissue · Apolipoprotein E · Fat droplet · Melanocortin · Melanocortin 3 receptor · Melanocortin 4 receptor · N acetyl alpha intermedin[4-10]cyclo[4 norleucine 5 aspartic acid 7 [3 (2 naphthyl)alanine] 10 lysinamide] · Triacylglycerol · Uncoupling protein 1 · Animal experiment · Animal tissue · Body fat · Brown adipose tissue · Central melanocortin system · Controlled study · Dyslipidemia · Endocrine system · Fat mass · Fatty liver · Female · Food intake · Hormone inhibition · Lipid analysis · Lipid oxidation · Lipid storage · Mouse · Nonhuman · Obesity · Signal transduction · Weight gain · Biomedical Innovation · Healthy Living · Life · MHR - Metabolic Health Research · ELSS - Earth, Life and Social Sciences
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The melanocortin system is an important regulator of energy balance, and melanocortin 4 receptor (MC4R) deficiency is the most common monogenic cause of obesity. We investigated whether the relationship between melanocortin system activity and energy expenditure (EE) is mediated by brown adipose tissue (BAT) activity. Therefore, female APOE∗3-Leiden.CETP transgenic mice were fed a Western-type diet for 4 weeks and infused intracerebroventricularly with the melanocortin 3/4 receptor (MC3/4R) antagonist SHU9119 or vehicle for 2 weeks. SHU9119 increased food intake (+30%) and body fat (+50%) and decreased EE by reduction in fat oxidation (-42%). In addition, SHU9119 impaired the uptake of VLDL-TG by BAT. In line with this, SHU9119 decreased uncoupling protein-1 levels in BAT (-60%) and induced large intracellular lipid droplets, indicative of severely disturbed BAT activity. Finally, SHU9119-treated mice pair-fed to the vehicletreated group still exhibited these effects, indicating that MC4R inhibition impairs BAT activity independent of food intake. These effects were not specific to the APOE∗3-Leiden.CETP background as SHU9119 also inhibited BAT activity in wild-type mice. We conclude that inhibition of central MC3/4R signaling impairs BAT function, which is accompanied by reduced EE, thereby promoting adiposity. We anticipate that activation of MC4R is a promising strategy to combat obesity by increasing BAT activity. Chemicals/CAS: melanocortin 3 receptor, 189235-81-2; melanocortin 4 receptor, 201099-18-5; n acetyl alpha intermedin[4-10]cyclo[4 norleucine 5 aspartic acid 7 [3 (2 naphthyl)alanine] 10 lysinamide], 168482-23-3
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[PDF]
[Abstract]
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9 |
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Sympathetic nervous system control of triglyceride metabolism: Novel concepts derived from recent studies
Abstract Important players in triglyceride (TG) metabolism include the liver (production), white adipose tissue (WAT) (storage), heart and skeletal muscle (combustion to generate ATP), and brown adipose tissue (BAT) (combustion toward heat), the collective action of which determine plasma TG levels. Interestingly, recent evidence points to a prominent role of the hypothalamus in TG metabolism through innervating the liver, WAT, and BAT mainly via sympathetic branches of the autonomic nervous system. Here, we review the recent findings in the area of sympathetic control of TG metabolism. Various neuronal populations, such as neuropeptide Y (NPY)-expressing neurons and melanocortin- expressing neurons, as well as peripherally produced hormones (i.e., GLP-1, leptin, and insulin), modulate sympathetic outfl ow from the hypothalamus toward target organs and thereby infl uence peripheral TG metabolism. We conclude that sympathetic stimulation in general increases lipolysis in WAT, enhances VLDL-TG production by the liver, and increases the activity of BAT with respect to lipolysis of TG, followed by combustion of fatty acids toward heat. Moreover, the increased knowledge about the involvement of the neuroendocrine system in TG metabolism presented in this review offers new therapeutic options to fight hypertriglyceridemia by specifically modulating sympathetic nervous system outfl ow toward liver, BAT, or WAT .-Geerling, J. J., M. R. Boon, S. Kooijman, E. T. Parlevliet, L. M. Havekes, J. A. Romijn, I. M. Meurs, and P. C. N. Rensen. Sympathetic nervous system control of triglyceride metabolism: novel concepts derived from recent studies. Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc..
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[PDF]
[Abstract]
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10 |
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Dynamics of insulin signalling in liver during hyperinsulinemic euglycaemic clamp conditions in vivo and the effects of high-fat feeding in male mice
article |
2007
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Author: |
Korsheninnikova, E.
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Voshol, P.J.
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Baan, B.
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Zon, G.C.M. van der
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Havekes, L.M.
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Romijn, J.A.
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Maassen, J.A.
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Ouwens, D.M.
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Keywords: |
Biology · Biomedical Research · Hepatic glucose production · High-fat diet · Insulin resistance · Insulin signalling · Liver · glucose · insulin · insulin receptor · phosphatidylinositol 3 kinase · protein kinase B · animal · article · blood · C57BL mouse · drug effect · fat intake · gene expression regulation · glucose clamp technique · hyperinsulinism · liver · male · metabolism · mouse · signal transduction · 1-Phosphatidylinositol 3-Kinase · Animals · Dietary Fats · Gene Expression Regulation, Enzymologic · Glucose · Glucose Clamp Technique · Hyperinsulinism · Insulin · Liver · Male · Mice · Mice, Inbred C57BL · Proto-Oncogene Proteins c-akt · Receptor, Insulin · Signal Transduction · Mus
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Insulin is an important regulator of hepatic carbohydrate, lipid, and protein metabolism, and the regulation of these processes by insulin is disturbed under conditions of insulin resistance and type 2 diabetes. Despite these alterations, the impact of insulin resistance on insulin signalling in the liver is not well defined. Variations in time and dose of insulin stimulation as well as plasma glucose levels may underlie this. The present study aimed at determining the dynamics of activation of hepatic insulin signalling in vivo at insulin concentrations resembling those achieved after a meal, and addressing the effects of high-fat feeding. An unexpected finding of this study was the biphasic activation pattern of the IRS-PI3K-PKB/Akt pathway. Our findings indicate that the first burst of activation contributes to regulation of glucose metabolism. The physiological function of the second peak is still unknown, but may involve regulation of protein synthesis. Finally, high-fat feeding caused hepatic insulin resistance, as illustrated by a reduced suppression of hepatic glucose production. A sustained increased phosphorylation of the serine/threonine kinases p70S6kinase and Jun N-terminal kinase in the absence of insulin may underlie the abrogated phosphorylation of the IRS proteins and their downstream targets.
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[Abstract]
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11 |
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Dietary sphingolipids lower plasma cholesterol and triacylglycerol and prevent liver steatosis
The prevalence of dyslipidemia and obesity resulting from excess energy intake and physical inactivity is increasing. The liver plays a pivotal role in the systemic lipid homeostasis. Effective, natural dietary interventions that lower plasma lipids and promote liver health are needed. APOE*3Leiden mice were fed a Western-type diet, supplemented with different sphingolipids, to determine their effect on plasma lipids and liver steatosis. Hepatic lipid levels and lipid-related gene expression were also determined. Dietary sphingolipids dose-dependently lowered both plasma cholesterol (C) and triglycerides (TG) in APOE*3Leiden mice. 1 % Phytosphingosine (PS) reduced C and TG by 57 and 58%, respectively. PS (a) decreased the absorption of dietary C and free fatty acid but did not affect the intestinal TG lipolysis, (b) increased hepatic VLDL-TG production whereas plasma lipolysis was not affected; and (c) increased the hepatic uptake of VLDL remnants. Hepatic mRNA levels indicated enhanced hepatic lipid synthesis and VLDL and LDL uptake. Livers of PS (1%) fed mice were lighter (-22%), less pale, and contained less cholesteryl ester (-61%) and TG (-56%). Furthermore, markers for liver inflammation (SAA) and liver damage (ALAT) were decreased by 74% and 79%, respectively in PS-fed mice. Sphingolipids lower plasma C and TG and protect the liver from fat- and cholesterol-induced steatosis. In a preliminary small double-blind cross-over study with six middle-aged slightly overweight male volunteers the daily supplementation of one gram of PS to the diet resulted in a -9.8% (p = 0.0074) and - 13.2% (p = 0.0002) reduction of total C and LDL-C, respectively. The C/HDL-C ratio was not significantly affected (p = 0.0571). Due to the relatively low pre-study levels of TG in the human volunteers, and the individual variability of TG levels, the TG lowering in humans was not significant in this first small study, but per individual there was a clear trend in TG lowering. © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.
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[Abstract]
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12 |
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Effects of high fat diet on the basal activity of the hypothalamus- pituitary-adrenal axis in mice: A systematic review
Hypothalamus-pituitary-adrenal-axis activity is suggested to be involved in the pathophysiology of the metabolic syndrome. In diet-induced obesity mouse models, features of the metabolic syndrome are induced by feeding high fat diet. However, the models reveal conflicting results with respect to the hypothalamus-pituitary-adrenal-axis activation. The aim of this review was to assess the effects of high fat feeding on the activity of the hypothalamus-pituitary-adrenal-axis in mice. PubMed, EMBASE, Web of Science, the Cochrane database, and Science Direct were electronically searched and reviewed by 2 individual researchers. We included only original mouse studies reporting parameters of the hypothalamus-pituitary-adrenal-axis after high fat feeding, and at least 1 basal corticosterone level with a proper control group. Studies with adrenalectomized mice, transgenic animals only, high fat diet for less than 2 weeks, or other interventions besides high fat diet, were excluded. 20 studies were included. The hypothalamus-pituitary-adrenal-axis evaluation was the primary research question in only 5 studies. Plasma corticosterone levels were unchanged in 40%, elevated in 30%, and decreased in 20% of the studies. The effects in the peripheral tissues and the central nervous system were also inconsistent. However, major differences were found between mouse strains, experimental conditions, and the content and duration of the diets. This systematic review demonstrates that the effects of high fat feeding on the basal activity of the hypothalamus-pituitary-adrenal-axis in mice are limited and inconclusive. Differences in experimental conditions hamper comparisons and accentuate the need for standardized evaluations to discern the effects of diet-induced obesity on the hypothalamus-pituitary-adrenal-axis. © Georg Thieme Verlag KG Stuttgart.
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[Abstract]
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13 |
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Stimulatory effect of insulin on glucose uptake by muscle involves the central nervous system in insulin-sensitive mice
OBJECTIVE - Insulin inhibits endogenous glucose production (EGP) and stimulates glucose uptake in peripheral tissues. Hypothalamic insulin signaling is required for the inhibitory effects of insulin on EGP. We examined the contribution of central insulin signaling on circulating insulin-stimulated tissue-specific glucose uptake. RESEARCH DESIGN AND METHODS - Tolbutamide, an inhibitor of ATP-sensitive K+ channels (KATP channels), or vehicle was infused into the lateral ventricle in the basal state and during hyperinsulinemic-euglycemic conditions in postabsorptive, chow-fed C57Bl/6J mice and in postabsorptive C57Bl/6J mice with dietinduced obesity. Whole-body glucose uptake was measured by D-[14C]glucose kinetics and tissue-specific glucose uptake by 2-deoxy-D-[3H]glucose uptake. RESULTS - During clamp conditions, intracerebroventricular administration of tolbutamide impaired the ability of insulin to inhibit EGP by ~20%. In addition, intracerebroventricular tolbutamide diminished insulin-stimulated glucose uptake in muscle (by ~59%) but not in heart or adipose tissue. In contrast, in insulin-resistant mice with diet-induced obesity, intracerebroventricular tolbutamide did not alter the effects of insulin during clamp conditions on EGP or glucose uptake by muscle. CONCLUSIONS - Insulin stimulates glucose uptake in muscle in part through effects via KATP channels in the central nervous system, in analogy with the inhibitory effects of insulin on EGP. Highfat diet-induced obesity abolished the central effects of insulin on liver and muscle. These observations stress the role of central insulin resistance in the pathophysiology of diet-induced insulin resistance. © 2011 by the American Diabetes Association.
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[Abstract]
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14 |
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The dopamine receptor D2 agonist bromocriptine inhibits glucose-stimulated insulin secretion by direct activation of the α2-adrenergic receptors in beta cells
article |
2010
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Author: |
Leeuw van Weenen, J.E. de
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Parlevliet, E.T.
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Maechler, P.
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Havekes, L.M.
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Romijn, J.A.
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Ouwens, D.M.
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Pijl, H.
·
Guigas, B.
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Keywords: |
Biology · Biomedical Research · α2-Adrenergic receptor · Bromocriptine · Dopamine receptor · INS-1E cells · Insulin secretion · Mice · alpha 2 adrenergic receptor · bromocriptine · cyclic AMP · domperidone · dopamine 2 receptor stimulating agent · G protein coupled receptor · glucose · insulin · placebo · yohimbine · analytic method · animal cell · animal experiment · article · cell line · cell membrane depolarization · cell membrane potential · controlled study · drug effect · glucose clamp technique · glucose tolerance test · hyperglycemia · in vitro study · in vivo study · inhibition kinetics · insulin release · male · mouse · nonhuman · pancreas islet beta cell · priority journal · Western blotting · Animals · Bromocriptine · Cell Line · Dopamine Agonists · Dose-Response Relationship, Drug · Glucose · Insulin · Insulin-Secreting Cells · Insulinoma · Male · Mice · Mice, Inbred C57BL · Rats · Receptors, Adrenergic, alpha · Receptors, Dopamine D2
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Treatment with the dopamine receptor D2 (DRD2) agonist bromocriptine improves metabolic features in obese patients with type 2 diabetes by a still unknown mechanism. In the present study, we investigated the acute effect of bromocriptine and its underlying mechanism(s) on insulin secretion both in vivo and in vitro. For this purpose, C57Bl6/J mice were subjected to an intraperitoneal glucose tolerance test (ipGTT) and a hyperglycemic (HG) clamp 60. min after a single injection of bromocriptine or placebo. The effects of bromocriptine on glucose-stimulated insulin secretion (GSIS), cell membrane potential and intracellular cAMP levels were also determined in INS-1E beta cells. We report here that bromocriptine increased glucose levels during ipGTT in vivo, an effect associated with a dose-dependent decrease in GSIS. During the HG clamp, bromocriptine reduced both first-phase and second-phase insulin response. This inhibitory effect was also observed in INS-1E beta cells, in which therapeutic concentrations of bromocriptine (0.5-50. nM) decreased GSIS. Mechanistically, neither cellular energy state nor cell membrane depolarization was affected by bromocriptine whereas intracellular cAMP levels were significantly reduced, suggesting involvement of G-protein-coupled receptors. Surprisingly, the DRD2 antagonist domperidone did not counteract the effect of bromocriptine on GSIS, whereas yohimbine, an antagonist of the α2-adrenergic receptors, completely abolished bromocriptine-induced inhibition of GSIS. In conclusion, acute administration of bromocriptine inhibits GSIS by a DRD2-independent mechanism involving direct activation of the pancreatic α2-adrenergic receptors. We suggest that treatment with bromocriptine promotes beta cells rest, thereby preventing long-lasting hypersecretion of insulin and subsequent beta cell failure. © 2010 Elsevier Inc.
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[Abstract]
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15 |
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Dietary sphingolipids lower plasma cholesterol and triacylglycerol and prevent liver steatosis in APOE*3Leiden mice
article |
2006
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Author: |
Duivenvoorden, I.
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Voshol, P.J.
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Rensen, P.C.N.
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Duyvenvoorde, W. van
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Romijn, J.A.
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Emeis, J.J.
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Havekes, L.M.
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Nieuwenhuizen, W.F.
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Keywords: |
Health Biology · Food technology · APOE*3Leiden mice · Cholesterol · Free fatty acids · Sphingolipids · Steatosis · Triacylglycerol · alanine aminotransferase · amyloid · apolipoprotein E3 · cholesterol · cholesterol ester · fatty acid · messenger RNA · phytosphingosine · RNA · sphingolipid · triacylglycerol · apolipoprotein E · apolipoprotein E3 (Leidein) · very low density lipoprotein · animal experiment · animal model · animal tissue · article · cholesterol blood level · cholesterol metabolism · controlled study · diet supplementation · dose response · drug effect · drug structure · fatty liver · female · gene expression · hepatitis · lipogenesis · lipolysis · mouse · nonhuman · nutritional assessment · plasma clearance · prophylaxis · animal · blood · chemistry · cholesterol intake · enzymology · feces · genetics · intestine absorption · lipid metabolism · liver · metabolism · physiology · randomization · transgenic mouse · Animals · Apolipoprotein E3 · Apolipoproteins E · Cholesterol · Cholesterol, Dietary · Dose-Response Relationship, Drug · Fatty Acids, Nonesterified · Fatty Liver · Feces · Female · Gene Expression · Intestinal Absorption · Lipid Metabolism · Lipolysis · Lipoproteins, VLDL · Liver · Mice · Mice, Transgenic · Random Allocation · RNA · Sphingolipids · Triglycerides
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Background: The prevalence of dyslipidemia and obesity resulting from excess energy intake and physical inactivity is increasing. The liver plays a pivotal role in systemic lipid homeostasis. Effective, natural dietary interventions that lower plasma lipids and promote liver health are needed. Objective: Our goal was to determine the effect of dietary sphingolipids on plasma lipids and liver steatosis. Design: APOE*3Leiden mice were fed a Western-type diet supplemented with different sphingolipids. Body cholesterol and triacylglycerol metabolism as well as hepatic lipid concentrations and lipid-related gene expression were determined. Results: Dietary sphingolipids dose-dependently lowered both plasma cholesterol and triacylglycerol in APOE*3Leiden mice; 1% phytosphingosine (PS) reduced plasma cholesterol and triacylglycerol by 57% and 58%, respectively. PS decreased the absorption of dietary cholesterol and free fatty acids by 50% and 40%, respectively, whereas intestinal triacylglycerol lipolysis was not affected. PS increased hepatic VLDL-triacylglycerol production by 20%, whereas plasma lipolysis was not affected. PS increased the hepatic uptake of VLDL remnants by 60%. Hepatic messenger RNA concentrations indicated enhanced hepatic lipid synthesis and VLDL and LDL uptake. The net result of these changes was a strong decrease in plasma cholesterol and triacylglycerol. The livers of 1% PS-fed mice were less pale, 22% lighter, and contained 61% less cholesteryl ester and 56% less triacylglycerol than livers of control mice. Furthermore, markers of liver inflammation (serum amyloid A) and liver damage (alanine aminotransferase) decreased by 74% and 79%, respectively, in PS-fed mice. Conclusion: Sphingolipids lower plasma cholesterol and triacylglycerol and protect the liver from fat- and cholesterol-induced steatosis. © 2006 American Society for Nutrition.Chemicals / CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; amyloid, 11061-24-8; cholesterol, 57-88-5; phytosphingosine, 13552-11-9, 554-62-1; RNA, 63231-63-0; apolipoprotein E3 (Leidein); Apolipoprotein E3; Apolipoproteins E; Cholesterol, 57-88-5; Cholesterol, Dietary; Fatty Acids, Nonesterified; Lipoproteins, VLDL; RNA, 63231-63-0; Sphingolipids; Triglycerides
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[Abstract]
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The effects of high fat diet on the basal activity of the hypothalamus-pituitary-adrenal axis in mice
Alterations in hypothalamus-pituitary-adrenal (HPA) axis activity have been linked to the development of the metabolic syndrome (MetS). Common features of the MetS, like insulin resistance and obesity, are reproducibly induced by high fat diet (HFD) in animal models of diet-induced obesity. These models, hampered by methodological differences, reveal conflicting results with respect to HPA axis activation. This study was aimed to evaluate in detail nonstressed diurnal HPA axis activity in mice during obesity development. Male C57Bl/6J mice were fed high or low fat diet for 12 weeks. HPA axis activity was evaluated by plasma corticosterone concentrations (at 0700, 1200, and 1800 h), corticotropinreleasing hormone (CRH), and glucocorticoid receptor (GR) mRNA expression in the hippocampus, amygdala, and hypothalamus, and 11β-hydroxysteroid dehydrogenase type-1 and -2 (11β-HSD-1 and -2) expression in adipose tissue and liver. Within 1 week, the HFD induced obesity and decreased corticosterone levels at 1200 and 1800 h, which persisted throughout the experiment. Twelve weeks of HFD decreased CRH mRNA in the paraventricular nucleus (PVN) and amygdala and GR mRNA in the PVN at 0900 h. At 1800 h, CRH mRNA expression increased in the PVN and amygdala, and GR mRNA increased in the CA1 region. 11β-HSD-1 expressions decreased in gonadal, visceral, and subcutaneous adipose tissues at 0900 and 1800 h, whereas hepatic 11β-HSD-1 expression increased at 1800 h, whereas 11β-HSD-2 expression was unaffected. The HFD induces complex changes in the diurnal regulation of the different components of the HPA axis. These changes are not unequivocally characterized by increased, but rather by decreased HPA axis activity. © 2012 Society for Endocrinology.
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[Abstract]
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CETP expression reverses the reconstituted HDL-induced increase in VLDL
Human data suggest that reconstituted HDL (rHDL) infusion can induce atherosclerosis regression. Studies in mice indicated that rHDL infusion adversely affects VLDL levels, but this effect is less apparent in humans. This discrepancy may be explained by the fact that humans, in contrast to mice, express cholesteryl ester transfer protein (CETP). The aim of this study was to investigate the role of CETP in the effects of rHDL on VLDL metabolism by using APOE*3-Leiden (E3L) mice, a well-established model for human-like lipoprotein metabolism. At 1 h after injection, rHDL increased plasma VLDL-C and TG in E3L mice, but not in E3L mice cross-bred onto a human CETP background (E3L.CETP mice). This initial raise in VLDL, caused by competition between rHDL and VLDL for LPLmediated TG hydrolysis, was thus prevented by CETP. At 24 h after injection, rHDL caused a second increase in VLDL-C and TG in E3L mice, whereas rHDL had even decreased VLDL in E3L.CETP mice. This secondary raise in VLDL was due to increased hepatic VLDL-TG production. Collectively, we conclude that CETP protects against the rHDL-induced increase in VLDL. We anticipate that studies evaluating the anti-atherosclerotic efficacy of rHDL in mice that are naturally deficient for CETP should be interpreted with caution, and that treatment of atherogenic dyslipidemia by rHDL should not be combined with agents that aggressively reduce CETP activity. Copyright © 2011 by the American Society for Biochemistry and Molecular Biology, Inc.
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[Abstract]
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The effect of dietary phytosphingosine on cholesterol levels and insulin sensitivity in subjects with the metabolic syndrome
Background: Sphingolipids, like phytosphingosine (PS) are part of cellular membranes of yeasts, vegetables and fruits. Addition of PS to the diet decreases serum cholesterol and free fatty acid (FFA) levels in rodents and improves insulin sensitivity.Objective:To study the effect of dietary supplementation with PS on cholesterol and glucose metabolism in humans. Methods: Twelve men with the metabolic syndrome (MetS) (according to the International Diabetes Federation (IDF) criteria; age 512 years (means.e.m.); body mass index (BMI) 321 kg/m 2) were randomly assigned to 4 weeks of PS (500 mg twice daily) and 4 weeks of placebo (P) in a double-blind cross-over study, with a 4-week wash-out period between both interventions. At the end of each intervention anthropometric measures and serum lipids were measured and an intravenous glucose tolerance test (IVGTT) was performed. Results: Phytosphingosine did not affect body weight and fat mass compared with P. PS decreased serum total cholesterol (5.10.3 (PS) vs 5.40.3 (P) mmol/l; P0.05) and low-density lipoprotein (LDL)-cholesterol levels (3.10.3 (PS) vs 3.40.3 (P) mmol/l; P0.05), whereas it did not alter serum triglyceride and high-density lipoprotein (HDL)-cholesterol levels. In addition, PS lowered fasting plasma glucose levels (6.20.3 (PS) vs 6.50.3 (P) mmol/l; P0.05). PS increased the glucose disappearance rate (K-value) by 9.9% during the IVGTT (0.910.06 (PS) vs 0.820.05 (P) %/min; P0.05) at similar insulin levels, compared with P, thus implying enhanced insulin sensitivity. PS induced only minor gastrointestinal side effects. Conclusion: Dietary supplementation of PS decreases plasma cholesterol levels and enhances insulin sensitivity in men with the MetS. © 2010 Macmillan Publishers Limited All rights reserved.
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[Abstract]
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Oxyntomodulin ameliorates glucose intolerance in mice fed a high-fat diet
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Intracerebroventricular administration of neuropeptide Y induces hepatic insulin resistance via sympathetic innervation
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2008
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Author: |
Hoek, A.M. van den
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Heijningen, C. van
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Schröder - Elst, J.P. van der
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Ouwens, D.M.
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Havekes, L.M.
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Romijn, J.A.
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Kalsbeek, A.
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Pijl, H.
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Keywords: |
corticosterone · glucose · insulin · liothyronine · neuropeptide Y · testosterone · thyroxine · antidiabetic agent · animal experiment · animal model · animal tissue · article · controlled study · drug mechanism · glucose metabolism · hormone blood level · insulin resistance · liver · male · nonhuman · parasympathetic innervation · priority journal · rat · sympathetic innervation · adrenergic system · animal · drug effect · glucose blood level · glucose clamp technique · hyperinsulinism · innervation · intracerebroventricular drug administration · metabolism · pathophysiology · physiology · sympathectomy · vagotomy · Wistar rat · Animals · Blood Glucose · Glucose Clamp Technique · Hyperinsulinism · Hypoglycemic Agents · Injections, Intraventricular · Insulin · Insulin Resistance · Liver · Male · Neuropeptide Y · Parasympathectomy · Rats · Rats, Wistar · Sympathectomy · Sympathetic Nervous System
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OBJECTIVE-We recently showed that intracerebroventricular infusion of neuropeptide Y (NPY) hampers inhibition of endogenous glucose production (EGP) by insulin in mice. The down stream mechanisms responsible for these effects of NPY remain to be elucidated. Therefore, the aim of this study was to establish whether intracerebroventricular NPY administration modulates the suppressive action of insulin on EGP via hepatic sympathetic or parasympathetic innervation. RESEARCH DESIGN AND METHODS-The effects of a continuous intracerebroventricular infusion of NPY on glucose turnover were determined in rats during a hyperinsulinemic- euglycemic clamp. Either rats were sham operated, or the liver was sympathetically (hepatic sympathectomy) or parasympa- thetically (hepatic parasympathectomy) denervated. RESULTS Sympathectomy or parasympathectomy did not affect the capacity of insulin to suppress EGP in intracerebroven- tricular vehicle-infused animals (50±8 vs. 49±6 vs. 55± 6%, in hepatic sympathectomy vs. hepatic parasympathectomy vs. sham, respectively). Intracerebroventricular infusion of NPY significantly hampered the suppression of EGP by insulin in sham-denervated animals (29±9 vs. 55±6% for NPY/sham vs. vehicle/sham, respectively, P = 0.038). Selective sympathetic denervation of the liver completely blocked the effect of intrace- rebroventricular NPY administration on insulin action to suppress EGP (NPY/hepatic sympathectomy, 57 ±7%), whereas selective parasympathetic denervation had no effect (NPY/hepatic parasympathectomy, 29±7%). CONCLUSIONS-Intracerebroventricular administration of NPY acutely induces insulin resistance of EGP via activation of sympathetic output to the liver. © 2008 by the American Diabetes Association.
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[Abstract]
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