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1 Efficient transformation system for Propionibacterium freudenreichii based on a novel vector
article 2001    
Author: Jore, J.P.M. · Luijk, N. van · Luiten, R.G.M. · Werf, M.J. van der · Pouwels, P.H.
Keywords: Environment · DNA Restriction-Modification Enzymes · Electroporation · Escherichia coli · Genetic Vectors · Molecular Sequence Data · Plasmids · Propionibacterium · Sequence Analysis, DNA · Transformation, Bacterial
[PDF] [Abstract]

2 Expression of Bacillus subtilis levanase gene in Lactobacillus plantarum and Lactobacillus casei
article 1995    
Author: Wanker, E. · Leer, R.J. · Pouwels, P.H. · Schwab, H.
Keywords: Nutrition · Bacillus subtilis · Comparative Study · Genes, Bacterial · Glycoside Hydrolases · Inulin · Lactobacillus · Lactobacillus casei · Plasmids · Selection (Genetics) · Support, Non-U.S. Gov't · Transformation, Genetic
[Abstract]

3 The construction of new vehicles for the cloning of transcription termination signals
article 1981    
Author: Enger-Valk, B.E. · Rotterdam, J. van · Pouwels, P.H.
Keywords: Biology · Animal experiment · Genetic code · Genetic transcription · Heredity · Molecular cloning · Plasmid · Base Sequence · Cloning, Molecular · DNA Restriction Enzymes · DNA, Recombinant · Escherichia coli · Genes · Genotype · Mutation · Operon · Plasmids · Support, Non-U.S. Gov't · Transcription, Genetic · Tryptophan
[Abstract]

4 Identification of resistance and virulence factors in an epidemic Enterobacter hormaechei outbreak strain
article 2009    
Author: Paauw, A. · Caspers, M.P.M. · Leverstein-van Hall, M.A. · Schuren, F.H.J. · Montijn, R.C. · Verhoef, J. · Fluit, A.C.
Keywords: Biology · Biotechnology · Bacterial DNA · Outer membrane protein · Virulence factor · Bacterial strain · Bacterial virulence · Bacterium isolate · DNA fragmentation · Enterobacter cloacae · Enterobacter hormaechei · Epidemic · Genetic marker · Genetic resistance · Nonhuman · Pathogenicity island · Plasticity · Salmonella enterica · Enterobacteriaceae infection · Genetics · Isolation and purification · Metabolism · Microbiology · Bacteria (microorganisms) · Salmonella enterica · Bacterial Proteins · Disease Outbreaks · Enterobacter · Enterobacteriaceae Infections · Netherlands · Plasmids · Virulence Factors
[Abstract]

5 Genetic analysis of acidocin B : a novel bacteriocin produced by Lactobacillus acidophilus
article 1995    
Author: Leer, R.J. · Vossen, J.M.B.M. van der · Giezen, M. van · Noort, J.M. van · Pouwels, P.H.
Keywords: Biology · Amino Acid Sequence · Bacteriocins · Base Sequence · Chromatography, High Pressure Liquid · Cloning, Molecular · Clostridium · Conserved Sequence · DNA, Bacterial · Genes, Bacterial · Lactobacillus · Lactobacillus acidophilus · Molecular Sequence Data · Multigene Family · Open Reading Frames · Plasmids · Protein Sorting Signals · Restriction Mapping · Support, Non-U.S. Gov't · Transformation, Bacterial
[Abstract]

6 Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon
article 1999    
Author: Martínez, B. · Fernández, M. · Suárez, J.E. · Rodríguez, A.
Keywords: Nutrition · Bacteriocin · Lactococcin 972 · Lactococcus lactis · Plasmid-encoded genes · Lactococcin · Bacterial gene · Gene expression · Lactococcus lactis · Nonhuman · Nucleotide sequence · Operon · Plasmid · Priority journal · Protein synthesis · Structural gene · Amino Acid Sequence · Bacteriocins · Base Sequence · Blotting, Northern · Electrophoresis, Polyacrylamide Gel · Genes, Bacterial · Lactococcus lactis · Molecular Sequence Data · Operon · Plasmids · RNA, Bacterial · Time Factors
[Abstract]

7 Transport of D-xylose in Lactobacillus pentosus, Lactobacillus casei, and Lactobacillus plantarum: Evidence for a mechanism of facilitated diffusion via the phosphoenolpyruvate:mannose phosphotransferase system
article 1999    
Author: Chaillou, S. · Pouwels, P.H. · Postma, P.W.
Keywords: Nutrition · Mannose · Phosphoenolpyruvate sugar phosphotransferase · Xylose · Bacterial growth · Carbohydrate transport · Diffusion · Enzyme mechanism · Fermentation · Lactobacillus · Lactobacillus casei · Lactobacillus pentosus · Lactobacillus plantarum · Nonhuman · Priority journal · Adenosine Triphosphate · Biological Transport, Active · Carbon Radioisotopes · Diffusion · Genetic Complementation Test · Kinetics · Lactobacillus casei · Mannose · Mutation · Phosphoenolpyruvate · Phosphoenolpyruvate Sugar Phosphotransferase System · Plasmids · Species Specificity · Transformation, Genetic · Xylose
[Abstract]

8 Expression and secretion of defined cutinase variants by Aspergillus awamori
article 1998    
Author: Gemeren, I.A. van · Beijersbergen, A. · Hondel, C.A.M.J.J. van den · Verrips, C.T.
Keywords: Nutrition · Cutinase · Aspergillus awamori · Endoplasmic reticulum · Enzyme synthesis · Gene expression · Mutagenesis · Nonhuman · Saccharomyces cerevisiae · Secretion · Aspergillus · Blotting, Western · Carboxylic Ester Hydrolases · Electrophoresis, Polyacrylamide Gel · Fungal Proteins · Gene Expression Regulation, Fungal · HSP70 Heat-Shock Proteins · Mutagenesis, Site-Directed · Plasmids · Precipitin Tests · Transformation, Genetic · Variation (Genetics) · Aspergillus awamori · Fungi · Fusarium · Fusarium solani · Saccharomyces cerevisiae
[Abstract]

9 Functional expression in Lactobacillus plantarum of xylP encoding the isoprimeverose transporter of Lactobacillus pentosus
article 1998    
Author: Chaillou, S. · Postma, P.W. · Pouwels, P.H.
Keywords: Nutrition · Carbohydrate · Carrier protein · Isoprimeverose · Unclassified drug · Xylose · Carbohydrate transport · Controlled study · Fermentation · Gene expression · Lactobacillus · Lactobacillus pentosus · Lactobacillus plantarum · Nonhuman · Priority journal · Proton motive force · Bacterial Proteins · Biological Transport · Carrier Proteins · Cloning, Molecular · Disaccharides · Kinetics · Lactobacillus · Malates · Operon · Plasmids · Recombinant Proteins · Restriction Mapping · Symporters · Xylose
[Abstract]

10 A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium
article 1996    
Author: Argnani, A. · Leer, R.J. · Luijk, N. van · Pouwels, P.H.
Keywords: Nutrition · Electrotransformation · Plasmid vector · Plasmid dna · Bacterium competence · Vontrolled study · Expression vector · Genetic transformation · Lactobacillus bifidus · Methodology · Nonhuman · Priority journal · Amino Acid Sequence · Bifidobacterium · Corynebacterium · DNA Replication · Electroporation · Genes, Bacterial · Genetic Vectors · Lactobacillus · Lactococcus · Molecular Sequence Data · Plasmids · Reproducibility of Results · Sequence Homology, Amino Acid · Species Specificity · Transformation, Genetic
[Abstract]

11 Bifidobacteria : genetic modification and the study of their role in the colon
article 2001    
Author: Werf, M. van der · Venema, K.
Keywords: Nutrition · In vitro gastrointestinal model · Prebiotics · Transformation · Probiotic agent · Bacterium transformation · Biological model · Colon flora · DNA modification · DNA vector · Gastrointestinal tract · Genetic transfection · Health promotion · Human · Nonhuman · Plasmid · Small intestine · Stomach · Bifidobacterium · Colon · Genetic Engineering · Genetic Vectors · Health Promotion · Humans · Models, Biological · Plasmids · Probiotics · Transfection · Bacteria (microorganisms) · Bifidobacterium
[Abstract]

12 Functional analysis of three plasmids from Lactobacillus plantarum
article 2005    
Author: Kranenburg, R. van · Golic, N. · Bongers, R. · Leer, R.J. · Vos, W.M. de · Siezen, R.J. · Kleerebezem, M.
Keywords: Biology · Biotechnology · Biomarkers · Genes · Plants (botany) · Functional analysis · Bacteria · Carnobacterium maltaromaticum · Arsenates · Arsenites · Base Sequence · Chromosome Mapping · DNA Replication · DNA, Bacterial · Drug Resistance, Bacterial · Gene Dosage · Genes, Bacterial · Lactobacillus plantarum · Molecular Sequence Data · Plasmids · Replicon · Sequence Homology, Nucleic Acid · Transfection · Bacillus subtilis · Carnobacterium maltaromaticum · Lactobacillus plantarum · Lactococcus lactis · Lactococcus plantarum
[PDF] [Abstract]

13 Agrobacterium-mediated transformation as a tool for functional genomics in fungi
article 2005    
Author: Michielse, C.B. · Hooykaas, P.J.J. · Hondel, C.A.M.J.J. van den · Ram, A.F.J.
Keywords: Biology · Analytical research · Agrobacterium tumefaciens · Fungal transformation · Insertional mutagenesis · Targeted mutagenesis · bacterial DNA · DNA integration · DNA synthesis · DNA transfer · functional genomics · fungal genetics · fungal strain · gene expression regulation · gene targeting · genetic transformation · molecular genetics · nonhuman · priority journal · review · Rhizobium · Rhizobium radiobacter · Bacterial Proteins · Base Sequence · DNA, Fungal · Genome, Fungal · Genomics · Mutagenesis, Insertional · Mutagenesis, Site-Directed · Plasmids · Rhizobium · Saccharomyces cerevisiae · Agrobacterium · Agrobacterium tumefaciens · Fungi
[Abstract]

14 A rapid and safe plasmid isolation method for efficient engineering of recombinant lactobacilli expressing immunogenic or tolerogenic epitopes for oral administration
article 1999    
Author: Maassen, C.B.M.
Keywords: Biology · EAE · Electroporation · Lactic acid bacteria · Mucosal tolerance · Oral vaccination · Plasmid extraction · Administration, Oral · Bacterial Proteins · Deoxyribonuclease BamHI · Deoxyribonucleases, Type II Site-Specific · DNA, Bacterial · Genetic Engineering · Immune Tolerance · Immunodominant Epitopes · Lactobacillus · Lactobacillus casei · Plasmids · Recombination, Genetic · Transformation, Genetic · Bacteria (microorganisms) · lactobacilli casei · Lactobacillus casei · Posibacteria
[Abstract]

15 An improved colony-PCR method for filamentous fungi for amplification of pcr-fragments of several kilobases
article 1998    
Author: Zeijl, C.M.J. van · Kamp, E.H.M. van de · Punt, P.J. · Selten, G.C.M. · Hauer, B. · Gorcom, R.F.M. van · Hondel, C.A.M.J.J. van den
Keywords: Nutrition · Colony-PCR method · Filamentous fungi · Rapid screening · Fungi · Colony-PCR method · Filamentous fungi · Biotechnology · Aspergillus niger · Bacterium transformation · Fungus · Gene disruption · Genetic transformation · Mycelium · Nonhuman · Plasmid · Polymerase chain reaction · Priority journal · Aspergillus niger · Cytochrome P-450 Enzyme System · DNA Mutational Analysis · DNA, Fungal · Enzymes · Glucan 1,4-alpha-Glucosidase · Plasmids · Transformation, Genetic · Aspergillus niger · Bacteria (microorganisms) · Bassiana · Beauveria · Cordyceps bassiana · Fungi
[Abstract]

16 Negative Regulation of Human Fibrinogen Gene Expression by Peroxisome Proliferator-activated Receptor α Agonists via Inhibition of CCAAT Box/Enhancer-binding Protein β
article 2001    
Author: Gervois, P. · Vu-Dac, N. · Kleemann, R. · Kockx, M. · Dubois, G. · Laine, B. · Kosykh, V. · Fruchart, J.C. · Kooistra, T. · Staels, B.
Keywords: Western blotting · Blotting, Northern · Blotting, Western · CCAAT-Enhancer-Binding Protein-alpha · Cell Line · Dose-Response Relationship, Drug · Down-Regulation · Fibrinogen · Hepatocytes · Humans · Inflammation · Interleukin-6 · Liver · Mutagenesis, Site-Directed · Nuclear Receptor Coactivator 2 · Peroxisome Proliferators · Plasmids · Precipitin Tests · Promoter Regions (Genetics) · Pyrimidines · Receptors, Cytoplasmic and Nuclear · Transcription Factors · Transcription, Genetic · Transfection · Tumor Cells, Cultured
[PDF] [Abstract]

17 Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori
article 2003    
Author: Lokman, B.C. · Joosten, V. · Hovenkamp, J. · Gouka, R.J. · Verrips, C.T. · Hondel, C.A.M.J.J. van den
Keywords: Biotechnology · Aspergillus awamori · Heme limitation · Heterologous protein production · Peroxidase · Cell culture · Enzyme kinetics · Fungi · Growth kinetics · Molecular weight · Western blot analysis · Biotechnology · fungal enzyme · peroxidase · xylan endo 1,3 beta xylosidase · arthromyces ramosus · article · Aspergillus awamori · culture medium · enzyme analysis · enzyme synthesis · fungus · molecular weight · nonhuman · priority journal · Western blotting · Aspergillus · Biotechnology · Blotting, Northern · Blotting, Southern · Fermentation · Heme · Mitosporic Fungi · Peroxidase · Plasmids · Promoter Regions (Genetics) · 'Arthromyces ramosus' · Aspergillus · Aspergillus awamori · Fungi
[Abstract]

18 Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi
article 1995    
Author: Gemeren, I.A. van · Musters, W. · Hondel, C.A.M.J.J. van den · Verrips, C.T.
Keywords: Nutrition · A. awamori · F. solani pisi cutinase · Filamentous fungi · Heterologous expression · S. cerevisiae · Synthetic gene construction · Complementary dna · Cutinase · Aspergillus · Biosynthesis · Controlled study · Fusarium solani · Gene expression · Nonhuman · Priority journal · Saccharomyces cerevisiae · Amino Acid Sequence · Aspergillus · Base Sequence · Biotechnology · Carboxylic Ester Hydrolases · DNA, Complementary · DNA, Fungal · Fusarium · Genes, Fungal · Genes, Synthetic · Genetic Vectors · Molecular Sequence Data · Plasmids · Recombinant Proteins · Saccharomyces cerevisiae · Support, Non-U.S. Gov't · Aspergillus · Aspergillus awamori · Fungi · Fusarium · Fusarium solani · Saccharomyces cerevisiae
[Abstract]

19 Organization and characterization of three genes involved in D-xylose catabolism in Lactobacillus pentosus
article 1991    
Author: Lokman, B.C. · Santen, P. van · Verdoes, J.C. · Kruse, J. · Leer, R.J. · Posno, M. · Pouwels, P.H.
Keywords: Biology · Amino acid comparison · D-xylose isomerase · D-xylulose kinase · NMR analysis · Regulatory protein · Xylose · Xylose isomerase · Amino acid sequence · Gene structure · Lactobacillus · Nonhuman · Nucleotide sequence · Priority journal · Sequence homology · Amino Acid Sequence · Bacillus subtilis · Base Sequence · Carbohydrate Epimerases · Cloning, Molecular · DNA, Bacterial · Genes, Bacterial · Lactobacillus · Magnetic Resonance Spectroscopy · Molecular Sequence Data · Multigene Family · Open Reading Frames · Phosphotransferases · Plasmids · Repressor Proteins · Restriction Mapping · Sequence Alignment · Sequence Homology, Nucleic Acid · Streptomyces · Xylose
[Abstract]

20 Examining the role of glutamic acid 183 in chloroperoxidase catalysis
article 2003    
Author: Yi, X. · Conesa, A. · Punt, P.J. · Hager, L.P.
Keywords: Biology · Biotechnology · Catalysis · Genetic engineering · Organic acids · X ray crystallography · Active sites · Enzymes · Chloride peroxidase · Clutamic acid · Histidine · Hydrogen peroxide · Natural product · Epoxidase · Oxidoreductase · Amino acid substitution · Animal cell · Asymmetric catalysis · Chlorination · Enzyme active site · Enzyme activity · Epoxidation · Genetic analysis · Hydrophobicity · Nonhuman · Plasmid · Site directed mutagenesis · X ray crystallography · Chemistry · Circular dichroism · Enzymology · Immunoblotting · Ion exchange chromatography · Isoelectric focusing · Metabolism · PH · polyacrylamide gel electrophoresis · Animalia · Aspergillus · Aspergillus niger · Catalase · Catalysis · Chloride Peroxidase · Chlorine · Chromatography, Ion Exchange · Circular Dichroism · Crystallography, X-Ray · Electrophoresis, Polyacrylamide Gel · Fungi · Glutamic Acid · Histidine · Hydrogen-Ion Concentration · Immunoblotting · Isoelectric Focusing · Mutation · Oxidoreductases · Plasmids
[PDF] [Abstract]

Search results also available in MS Excel format.

Showing 1 to 20 of 26 found. Next | Sort by date