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1 Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
article 2000    
Author: Krasevec, N. · Hondel, C.A.M.J.J. van den · Komel, R.
Keywords: Aspergillus niger · Heterologous protein secretion · hTNF-α · hTNF-α analogues · Glucan 1,4 alpha glucosidase · Tumor necrosis factor alpha · Amino acid sequence · Aspergillus niger · Biosynthesis · Gene expression · Gene fusion · Genetics · Human · Metabolism · Secretion · Amino Acid Sequence · Artificial Gene Fusion · Aspergillus niger · Gene Expression · Glucan 1,4-alpha-Glucosidase · Humans · Tumor Necrosis Factor-alpha
[Abstract]

2 Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein
article 1999    
Author: Santerre Henriksen, A.L. · Even, S. · Müller, C. · Punt, P.J. · Hondel, C.A.M.J.J. van den · Nielsen, J.
Keywords: Nutrition · Aspergillus niger · Chemostat · Glucoamylase promoter · Green fluorescent protein · Glucan 1,4 alpha glucosidase · Green fluorescent protein · Maltose · Xylose · Aspergillus niger · Continuous culture · Fungus culture · Growth rate · Mycelium · Nonhuman · Priority journal · Aspergillus niger · Genes, Reporter · Glucan 1,4-alpha-Glucosidase · Green Fluorescent Proteins · Luminescent Proteins · Promoter Regions (Genetics) · Aspergillus niger · Fungi
[Abstract]

3 A new method for screening and isolation of hypersecretion mutants in Aspergillus niger
article 2006    
Author: Weenink, X.O. · Punt, P.J. · Hondel, C.A.M.J.J. van den · Ram, A.F.J.
Keywords: Biology · Biotechnology · Enzymes · Fungi · Organic acids · Screening · Starch · Aspergillus niger · Extracellular proteins · Glucoamylase proteins · Mutagenesis · glucan 1,4 alpha glucosidase · laccase · detection method · fungus · microbiology · article · Aspergillus niger · catalysis · catalyst · correlation analysis · enzyme activity · fungus growth · fungus isolation · fungus mutant · isolation procedure · nonhuman · phenotype · Pleurotus ostreatus · protein synthesis · screening · Aspergillus niger · Biomass · Glucan 1,4-alpha-Glucosidase · Laccase · Mutation · Pleurotus · Protein Transport · Recombinant Proteins · Starch · Aspergillus niger · Fungi · Pleurotus ostreatus
[Abstract]

4 Expression of human α1-proteinase inhibitor in Aspergillus niger
article 2007    
Author: Karnaukhova, E. · Ophir, Y. · Trinh, L. · Dalal, N. · Punt, P.J. · Golding, B. · Shiloach, J.
Keywords: Biology · Biotechnology · alpha 1 antitrypsin · glucan 1,4 alpha glucosidase · glucan 1,4 alpha glucosidase 2 · recombinant alpha 1 antitrypsin · trypsin inhibitor · article · Aspergillus niger · biotechnological production · controlled study · enzyme linked immunosorbent assay · glycosylation · matrix assisted laser desorption ionization time of flight mass spectrometry · molecular weight · nonhuman · nucleotide sequence · polyacrylamide gel electrophoresis · protein degradation · protein determination · protein expression · protein synthesis · Western blotting · Aspergillus niger · Fungi
[Abstract]

5 The effect of organic nitrogen sources on recombinant glucoamylase production by Aspergillus niger in chemostat culture
article 2000    
Author: Swift, R.J. · Karandikar, A. · Griffen, A.M. · Punt, P.J. · Hondel, C.A.M.J.J. van den · Robson, G.D. · Trinci, A.P.J. · Wiebe, M.G.
Keywords: Biology · Aspergillus niger · Chemostat culture · Genetic instability · Glucoamylase · Nitrogen source · Recombinant protein · Alanine · Casamino acid · DNA · Fungal DNA · Fungal enzyme · Glucan 1,4 alpha glucosidase · Glucose · Methionine · Nitro derivative · Peptone · Aspergillus niger · Chemostat · Controlled study · Culture medium · DNA methylation · Enzyme metabolism · Enzyme synthesis · Fermentation · Fungal genetics · Fungus culture · Gene loss · Morphology · Nonhuman · PH · Priority journal · Strain difference · Aspergillus niger · Culture Media · Glucan 1,4-alpha-Glucosidase · Glucose · Hydrogen-Ion Concentration · Mutation · Nitrogen · Organic Chemicals · Recombinant Proteins · Aspergillus niger
[Abstract]

6 Analysis of the role of the gene bipA, encoding the major endoplasmic reticulum chaperone protein in the secretion of homologous and heterologous proteins in black Aspergilli
article 1998    
Author: Punt, P.J. · Gemeren, I.A. van · Drint-Kuijvenhoven, J. · Hessing, J.G.M. · Muijlwijk van - Harteveld, G.M. · Beijersbergen, A. · Verrips, C.T. · Hondel, C.A.M.J.J. van den
Keywords: Nutrition · Binding protein · Chaperone · Glucan 1,4 alpha glucosidase · Recombinant protein · Aspergillus awamori · Aspergillus niger · Endoplasmic reticulum · Fungal genetics · Gene fusion · Gene overexpression · Nonhuman · Promoter region · Protein secretion · Animals · Antibody Specificity · Artificial Gene Fusion · Aspergillus · Aspergillus niger · Blotting, Northern · Blotting, Western · Cloning, Molecular · Fungal Proteins · Gene Expression Regulation, Fungal · Genes, Fungal · Glucan 1,4-alpha-Glucosidase · HSP70 Heat-Shock Proteins · Rabbits · Recombinant Fusion Proteins · Aspergillus · Aspergillus awamori · Aspergillus niger · Fungi
[Abstract]

7 Substitution patterns in methylated potato starch as revealed from the structure and composition of fragments in enzymatic digests
article 2008    
Author: Steeneken, P.A.M. · Tas, A.C. · Woortman, A.J.J. · Sanders, P. · Mijland, P.J.H.C. · Weijs, L.G.R.de
Keywords: Nutrition · Analytical research · Enzymatic digestibility · MALDI · Methylation · Starch · Substitution pattern · Chemical reactions · Enzymes · Food additives · Monomers · Polymers · Structure (composition) · Sugars · Amyloglucosidase · Degree of polymerization · Enzymatic digestibility · Enzyme specificity · Granule structure · MALDI · Mass compositions · Methylation · Monomer compositions · Potato starch · Substitution pattern · Substitution patterns · Starch · amylase · carbohydrate · glucan 1,4 alpha glucosidase · oligosaccharide · potato starch · article · carbohydrate analysis · enzyme chemistry · enzyme specificity · fractionation · gas liquid chromatography · matrix assisted laser desorption ionization time of flight mass spectrometry · methylation · polymerization · priority journal · alpha-Amylases · Chromatography, Liquid · Glucan 1,4-alpha-Glucosidase · Methylation · Oligosaccharides · Solanum tuberosum · Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization · Starch · Substrate Specificity · Solanum tuberosum
[Abstract]

8 Endoplasmic reticulum stress leads to the selective transcriptional downregulation of the glucoamylase gene in Aspergillus niger
article 2004    
Author: Al-Sheikh, H. · Watson, A.J. · Lacey, G.A. · Punt, P.J. · MacKenzie, D.A. · Jeenes, D.J. · Pakula, T. · Penttilä, M. · Alcocer, M.J.C. · Archer, D.B.
Keywords: Biology · Biotechnology · actin · chaperone · dithiothreitol · gamma actin · glucan 1,4 alpha glucosidase · glyceraldehyde 3 phosphate dehydrogenase · protein HacA · unclassified drug · article · Aspergillus niger · bipa gene · down regulation · endoplasmic reticulum · fungal cell · fungal gene · fungal strain · gene expression · gene induction · genetic code · glaa gene · nonhuman · pdiA gene · priority journal · promoter region · protein folding · protein secretion · RNA splicing · RNA stability · stress · transcription regulation · upregulation · Aspergillus niger · Dithiothreitol · Down-Regulation · Endoplasmic Reticulum · Fungal Proteins · Gene Expression Regulation, Enzymologic · Gene Expression Regulation, Fungal · Glucan 1,4-alpha-Glucosidase · HSP70 Heat-Shock Proteins · Oligonucleotides, Antisense · Promoter Regions (Genetics) · Protein Folding · Transcription, Genetic · Aspergillus · Aspergillus niger · Fungi
[Abstract]

9 Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates
article 2005    
Author: Biesebeke, R. te · Record, E. · Biezen, N. van · Heerikhuisen, M. · Franken, A. · Punt, P.J. · Hondel, C.A.M.J.J. van den
Keywords: Biology · Biotechnology · Enzyme kinetics · Enzymes · Fermentation · Growth kinetics · Hydrolysis · Microorganisms · Substrates · Yeast · Amylase · Branching mutants · Hydrolytic enzymes · Solid substrates · Mutagenesis · amylase · carrier protein · glucan 1,4 alpha glucosidase · phosphatidylglycerol · phosphatidylinositol · proteinase · fungus · gene expression · protein · allele · article · Aspergillus oryzae · enzyme activity · enzyme synthesis · fermentation · fungal biomass · fungal genetics · fungal strain · fungus growth · fungus hyphae · fungus mutant · genetic code · hydrolysis · microscopy · nonhuman · protein secretion · seed kernel · solid · sporogenesis · wheat · wild type · Amylases · Aspergillus oryzae · Biomass · Fungal Proteins · Gene Deletion · Glucan 1,4-alpha-Glucosidase · Hyphae · Morphogenesis · Mutagenesis, Insertional · Mutation · Peptide Hydrolases · Phospholipid Transfer Proteins · Spores, Fungal · Triticum · Aspergillus oryzae · Fungi · Triticum aestivum
[Abstract]

10 Different control mechanisms regulate glucoamylase and protease gene transcription in Aspergillus oryzae in solid-state and submerged fermentation
article 2005    
Author: Biesebeke, R. te · Biezen, N. van · Vos, W.M. de · Hondel, C.A.M.J.J. van den · Punt, P.J.
Keywords: Biology · Biotechnology · Carbon · Control theory · Crops · Enzymes · Fermentation · Genes · Nitrogen · Gene expressions · Gene transcription · Glucomylase (glaB) · Solid-state fermentation (SSF) · Enzyme kinetics · carbon · glucan 1,4 alpha glucosidase · nitrogen · proteinase · fermentation · gene expression · protein · article · Aspergillus oryzae · controlled study · culture medium · fungus hyphae · gene control · gene expression · liquid culture · molecular mechanics · nonhuman · pH measurement · seed kernel · solid state fermentation · transcription regulation · wheat · Aspergillus oryzae · Biomass · Carbon · Fermentation · Gene Expression Profiling · Gene Expression Regulation, Fungal · Glucan 1,4-alpha-Glucosidase · Glucose · Hydrogen-Ion Concentration · Nitrogen · Peptide Hydrolases · RNA, Fungal · RNA, Messenger · Transcription, Genetic · Triticum · Carbon · Enzymatic Activity · Enzymes · Fermentation · Genes · Nitrogen · Wheat · Aspergillus oryzae · Triticum aestivum
[Abstract]

11 Optimization and stability of glucoamylase production by recombinant strains of Aspergillus niger in chemostat culture
article 1998    
Author: Withers, J.M. · Swift, R.J. · Wiebe, M.G. · Robson, G.D. · Punt, P.J. · Hondel, C.A.M.J.J. van den
Keywords: Nutrition · Aspergillus niger · Chemostat culture · Glucoamylase (GAM) · Protein secretion · Recombinant protein · Strain stability · Ammonium compounds · Batch cell culture · Chemostats · Genetic engineering · Glucose · Magnesium · Morphology · Phosphates · Physiology · Potassium · Sulfur compounds · Chemostat culture · Glucoamylase · Shake flask culture · Enzymes · glucan 1,4 alpha glucosidase · article · aspergillus niger · chemostat · enzyme stability · enzyme synthesis · fungus culture · fungus growth · genetic recombination · nonhuman · protein secretion · Aspergillus niger · Blotting, Western · Cell Culture Techniques · Cell Division · Culture Media · Fermentation · Glucan 1,4-alpha-Glucosidase · Glucose · Hydrogen-Ion Concentration · Polysaccharides · Recombinant Proteins · Temperature · Time Factors
[Abstract]

12 Expression of human lymphotoxin alpha in Aspergillus niger
article 2000    
Author: Krasevec, N. · Hondel, C.A.M.J.J. van de · Komel, R.
Keywords: Medicine · Geneeskunde · Pathology · Aspergillus niger · Codon usage · Heterologous protein secretion · LTα · TNFα · Complementary DNA · Lymphotoxin · Tumor necrosis factor alpha · Aspergillus niger · Codon usage · Conference paper · Controlled study · DNA sequence · Gene control · Gene fusion · Genetic transcription · Human · Nonhuman · Priority journal · Protein degradation · Protein secretion · Artificial Gene Fusion · Aspergillus niger · Blotting, Northern · Glucan 1,4-alpha-Glucosidase · Humans · Lymphotoxin-alpha · RNA, Messenger
[Abstract]

13 An expression system based on the promoter region of the Aspergillus awamori 1,4-bèta-endoxylanase A gene
article 1996    
Author: Gouka, R.J. · Hessing, J.G.M. · Punt, P.J. · Stam, H. · Musters, W. · Hondel, C.A.M.J.J. van den
Keywords: Nutrition · Enzyme · Glycosidase · Microbial enzyme · Controlled study · Gene expression regulation · Molecular cloning · Nonhuman · Promoter region · Aspergillus · Endo-1,4-beta Xylanases · Enzyme Induction · Gene Expression Regulation, Fungal · Genes, Reporter · Genetic Vectors · Glucan 1,4-alpha-Glucosidase · Glucuronidase · Promoter Regions (Genetics) · RNA, Fungal · RNA, Messenger · Transcription, Genetic · Xylose · Xylosidases · Aspergillus awamori · Aspergillus niger · Trixis
[Abstract]

14 Studies on the production of fungal peroxidases in Aspergillus niger
article 2000    
Author: Conesa, A. · Hondel, C.A.M.J.J. van den · Punt, P.J.
Keywords: Nutrition · Botany · Fungi · Fungal enzyme · Glucan 1,4 alpha glucosidase · Hemoglobin · Lignin peroxidase · Manganese peroxidase · Messenger RNA · Peroxidase · Recombinant protein · Aspergillus niger · Enzyme activity · Enzyme purification · Enzyme release · Enzyme synthesis · Gene overexpression · Nonhuman · Nucleotide sequence · Protein expression · Protein processing · Aspergillus niger · Blotting, Northern · Blotting, Western · Ferrous Compounds · Heme · Peroxidases · Phanerochaete · Recombinant Proteins · Transformation, Genetic · Aspergillus niger · Chrysosporium · Fungi · Phanerochaete chrysosporium
[Abstract]

15 An improved colony-PCR method for filamentous fungi for amplification of pcr-fragments of several kilobases
article 1998    
Author: Zeijl, C.M.J. van · Kamp, E.H.M. van de · Punt, P.J. · Selten, G.C.M. · Hauer, B. · Gorcom, R.F.M. van · Hondel, C.A.M.J.J. van den
Keywords: Nutrition · Colony-PCR method · Filamentous fungi · Rapid screening · Fungi · Colony-PCR method · Filamentous fungi · Biotechnology · Aspergillus niger · Bacterium transformation · Fungus · Gene disruption · Genetic transformation · Mycelium · Nonhuman · Plasmid · Polymerase chain reaction · Priority journal · Aspergillus niger · Cytochrome P-450 Enzyme System · DNA Mutational Analysis · DNA, Fungal · Enzymes · Glucan 1,4-alpha-Glucosidase · Plasmids · Transformation, Genetic · Aspergillus niger · Bacteria (microorganisms) · Bassiana · Beauveria · Cordyceps bassiana · Fungi
[Abstract]

16 Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles
article 2008    
Author: Yuan, X.-L. · Kaaij, R.M. van der · Hondel, C.A.M.J.J. van den · Punt, P.J. · Maarel, M.J.E.C. van der · Dijkhuizen, L. · Ram, A.F.J.
Keywords: Biology · Alpha-amylase · Alpha-glucan · Alpha-glucosidase · AmyR · Aspergillus niger · Cell wall · Glucoamylase · Maltose · Starch · Starch-binding domain · 1,4 alpha glucan branching enzyme · alpha glucosidase · amylase · cell enzyme · cell membrane protein · fungal protein · glucan 1,4 alpha glucosidase · glycosidase · glycosylphosphatidylinositol · maltose · protein amyr · starch · article · Aspergillus niger · carbohydrate metabolism · carbon source · controlled study · degradation · energy resource · enzymatic degradation · enzyme synthesis · gene expression profiling · gene identification · gene sequence · genetic transcription · genome analysis · microarray analysis · nonhuman · nucleotide sequence · priority journal · transcription regulation · alpha-Amylase · alpha-Glucosidases · Amino Acid Sequence · Aspergillus niger · Base Sequence · Conserved Sequence · Fungal Proteins · Gene Expression Profiling · Gene Expression Regulation, Fungal · Genome, Fungal · Genomics · Glucan 1,4-alpha-Glucosidase · Glycoside Hydrolases · Maltose · Molecular Sequence Data · Phylogeny · Trans-Activators · Transcription, Genetic · Aspergillus niger · Fungi
[Abstract]

17 The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins: Evidence for alternative processing of recombinant (fusion-) proteins
article 2003    
Author: Punt, P.J. · Drint-Kuijvenhoven, A. · Lokman, B.C. · Spencer, J.A. · Jeenes, D. · Archer, D.A. · Hondel, C.A.M.J.J. van den
Keywords: Biology · Biotechnology · Filamentous fungi · Fusion-proteins · Protease · Protein processing · Protein secretion · Secretion carrier · Biotechnology · Genes · Growth kinetics · Mutagenesis · Proteins · Cleavage sites · Genetic engineering · arginine · aspergillopepsin · fungal protein · furin · glucan 1,4 alpha glucosidase · hybrid protein · lysine · protein precursor · proteinase · recombinant protein · unclassified drug · allele · amino acid composition · article · Aspergillus niger · controlled study · fungal gene · fungal strain · fungus mutant · gene deletion · gene disruption · gene function · genetic code · genetic recombination · molecular cloning · nonhuman · nucleotide sequence · priority journal · protein degradation · protein processing · wild type · Aspergillus niger · Endopeptidases · Fungal Proteins · Furin · Gene Expression Regulation, Enzymologic · Gene Expression Regulation, Fungal · Protein Processing, Post-Translational · Recombinant Fusion Proteins · Trans-Activation (Genetics) · Aspergillus · Aspergillus niger · Fungi
[Abstract]

18 Quantitative iTRAQ secretome analysis of aspergillus niger reveals novel hydrolytic enzymes
article 2010    
Author: Adav, S.S. · Li, A.A. · Manavalan, A. · Punt, P. · Sze, S.K.
Keywords: Biology · Food and Chemical Risk Analysis · A. niger; lignocellulose · bioenergy and biorefinery · iTRAQ · secretome · alpha arabinofuranosidase · alpha glucosidase · alpha glucosidase c · aspartic proteinase · aspergillopepsin · beta mannosidase · biofuel · cellulase · cytochrome c oxidase · enzyme · glucan 1,4 alpha glucosidase · glucan synthase · glucose oxidase · glycosidase · hemicellulase · hydrolase · lignocellulose · membrane protein · peptidase · peroxidase · protein · protein translocating transporter protein · proteinase · secretome · tripeptidyl peptidase · unclassified drug · animal cell · animal experiment · article · Aspergillus niger · biofuel production · biomass · controlled study · culture medium · enzyme activity · enzyme release · enzyme specificity · enzyme synthesis · fermentation · hydrolysis · iTRAQ proteomics · liquid chromatography tandem mass spectrometry · mass spectrometry · mutant · nonhuman · pH · priority journal · protein analysis · proteomics · quantitative analysis · tandem mass spectrometry
[Abstract]

19 Characterization of a foldase, protein disulfide isomerase A, in the protein secretory pathway of Aspergillus niger
article 2000    
Author: Ngiam, C. · Jeenes, D.J. · Punt, P.J. · Hondel, C.A.M.J.J. van den · Archer, D.B.
Keywords: Nutrition · Egg white · Glucan 1,4 alpha glucosidase · Lysozyme · Messenger RNA · Microsome enzyme · Protein disulfide isomerase · Ribonuclease · Aspergillus niger · Endoplasmic reticulum · Enzyme activity · Enzyme analysis · Gene overexpression · Immunoblotting · Nonhuman · Protein folding · Protein induction · Protein secretion · Renaturation · Saccharomyces cerevisiae · Actins · Aspergillus niger · Calcimycin · Dithiothreitol · Down-Regulation · Endoplasmic Reticulum · Fungal Proteins · Gene Expression Regulation, Fungal · Heat-Shock Proteins · HSP70 Heat-Shock Proteins · Microsomes · Protein Denaturation · Protein Disulfide-Isomerase · Protein Folding · Recombinant Proteins · Ribonuclease, Pancreatic · RNA, Messenger · Saccharomyces cerevisiae · Transcription, Genetic · Transformation, Genetic · Aspergillus niger · Eukaryota · Saccharomyces cerevisiae
[PDF] [Abstract]

20 A fungal biofilm reactor based on metal structured packing improves the quality of a Gla::GFP fusion protein produced by Aspergillus oryzae
article 2015    
Author: Zune, Q. · Delepierre, A. · Gofflot, S. · Bauwens, J. · Twizere, J.C. · Punt, P.J. · Francis, F. · Toye, D. · Bawin, T. · Delvigne, F.
Keywords: Nutrition · Fungal biofilm · Bioreactor · Scale-up · Recombinant protein · Antigen-antibody reactions · Aspergillus · Biofilms · Biomass · Bioreactors · Electrophoresis · Enzyme activity · Fermentation · Fungi · Metabolites · Physiology · Recombinant proteins · Shear stress · Comparative analysis · Extracellular medium · Green fluorescent protein · Physiological mechanisms · Secondary metabolites · Solid-state fermentation · Submerged fermentation · Proteins · Amylase · Glucan 1,4 alpha glucosidase · Glucoamylase green fluorescent protein fusion protein · Green fluorescent protein · Hybrid protein · Unclassified drug · Fermentation · Fungus · Metal · Physiological response · Protein · Qualitative analysis · Recirculating system · Recombination · Secondary metabolite · Alkalinization · Amino acid sequence · Aspergillus niger · Aspergillus oryzae · Autolysis · Biofilm reactor · Biotransformation · Carbon source · Controlled study · Enzyme activity · Extracellular space · Fungal biomass · Fungal colonization · Fungus culture · Fungus growth · Glucose intake · Hydrolysis · Immersion · Immunodetection · Nonhuman · PH measurement · Protein analysis · Shear stress · Solid state fermentation · Submerged fermentation · Western blotting · Food and Nutrition · Healthy Living · Life · MSB - Microbiology and Systems Biology · ELSS - Earth, Life and Social Sciences
[Abstract]

Search results also available in MS Excel format.

Showing 1 to 20 of 21 found. Next | Sort by date