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1 Identification of multiple target sites for a glutathione conjugate on glutathione-S-transferase by matrix-assisted laser desorption/ionization mass spectrometry
article 1996    
Author: Jespersen, S. · Ploemen, J.H.T.M. · Bladeren, P.J. · Niessen, W.M.A. · Tjaden, U.R. · Greef, J. van der
Keywords: Nutrition · GSTCBQ target residues · Mapping site-specific mutagenesis · Binding Sites · Chloranil · Enzyme Inhibitors · Glutathione · Glutathione Transferase · Molecular Weight · Mutagenesis, Site-Directed · Peptide Mapping · Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
[Abstract]

2 A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori
article 1995    
Author: Gouka, R.J. · Hessing, J.G.M. · Stam, H. · Musters, W. · Hondel, C.A.M.J.J. van den
Keywords: Biology · Amino Acid Sequence · Aspergillus · Base Sequence · DNA, Fungal · Gene Transfer Techniques · Genes, Fungal · Genetic Vectors · Molecular Sequence Data · Mutagenesis, Site-Directed · Restriction Mapping · Transformation, Genetic · Aspergillus awamori · Aspergillus niger · Fungi
[Abstract]

3 Involvement of aspartic and glutamic residues in kringle-2 of tissue-type plasminogen activator in lysine binding, fibrin binding and stimulation of activity as revealed by chemical modification and oligonucleotide-directed mutagenesis
article 1990    
Author: Weening-Verhoeff, E.J.D. · Quax, P.H.A. · Leeuwen, R.T.J. van · Rehberg, E.F. · Marotti, K.R. · Verheijen, J.H.
Keywords: Health · Chemical modification · Ligand binding · Site directed mutagenesis · Amino Acid Sequence · Aspartic Acid · Base Sequence · Ethyldimethylaminopropyl Carbodiimide · Fibrin · Glutamates · Kinetics · Lysine · Molecular Sequence Data · Mutagenesis, Site-Directed · Mutation · Peptide Fragments · Plasminogen · Protein Engineering · Tissue Plasminogen Activator

4 Agrobacterium-mediated transformation as a tool for functional genomics in fungi
article 2005    
Author: Michielse, C.B. · Hooykaas, P.J.J. · Hondel, C.A.M.J.J. van den · Ram, A.F.J.
Keywords: Biology · Analytical research · Agrobacterium tumefaciens · Fungal transformation · Insertional mutagenesis · Targeted mutagenesis · bacterial DNA · DNA integration · DNA synthesis · DNA transfer · functional genomics · fungal genetics · fungal strain · gene expression regulation · gene targeting · genetic transformation · molecular genetics · nonhuman · priority journal · review · Rhizobium · Rhizobium radiobacter · Bacterial Proteins · Base Sequence · DNA, Fungal · Genome, Fungal · Genomics · Mutagenesis, Insertional · Mutagenesis, Site-Directed · Plasmids · Rhizobium · Saccharomyces cerevisiae · Agrobacterium · Agrobacterium tumefaciens · Fungi
[Abstract]

5 Structural and functional analysis of the S-layer protein crystallisation domain of Lactobacillus acidophilus ATCC 4356 : evidence for protein : protein interaction of two subdomains
article 2002    
Author: Smit, E. · Jager, D. · Martinez, B. · Tielen, F.J. · Pouwels, P.H.
Keywords: Biology · Bacteria (microorganisms) · Escherichia coli · Lactobacillus · Lactobacillus acidophilus · Alleles · Amino Acid Sequence · Bacterial Proteins · Binding Sites · Chromosomes, Bacterial · Crystallization · Epitopes · Escherichia coli · Genes, myc · Genetic Vectors · Lactobacillus acidophilus · Lactobacillus casei · Membrane Glycoproteins · Membrane Proteins · Molecular Sequence Data · Molecular Weight · Mutagenesis, Site-Directed · Peptide Fragments · Protein Binding · Protein Structure, Secondary · Protein Structure, Tertiary · Proto-Oncogene Proteins c-myc · Recombinant Proteins · Recombination, Genetic · Transformation, Bacterial · Vaccines
[Abstract]

6 Binding of human urokinase-type plasminogen activator to its receptor : Residues involved in species specificity and binding
article 1998    
Author: Quax, P.H.A. · Grimbergen, J.M. · Lansink, M. · Bakker, A.H.F. · Blatter, M.C. · Belin, D. · Hinsbergh, V.W.M. van · Verheijen, J.H.
Keywords: Health · Site-directed mutagenesis · Urokinase-type plasminogen activator · Urokinase-type plasminogen activator receptor · Amino Acid Sequence · Animals · Asparagine · Binding, Competitive · Cells, Cultured · Cricetinae · Endothelium, Vascular · Humans · Ligands · Mice · Molecular Sequence Data · Mutagenesis, Site-Directed · Receptors, Cell Surface · Sequence Homology, Amino Acid · Species Specificity · Swine · Tyrosine · Urinary Plasminogen Activator
[Abstract]

7 Expression and secretion of defined cutinase variants by Aspergillus awamori
article 1998    
Author: Gemeren, I.A. van · Beijersbergen, A. · Hondel, C.A.M.J.J. van den · Verrips, C.T.
Keywords: Nutrition · Cutinase · Aspergillus awamori · Endoplasmic reticulum · Enzyme synthesis · Gene expression · Mutagenesis · Nonhuman · Saccharomyces cerevisiae · Secretion · Aspergillus · Blotting, Western · Carboxylic Ester Hydrolases · Electrophoresis, Polyacrylamide Gel · Fungal Proteins · Gene Expression Regulation, Fungal · HSP70 Heat-Shock Proteins · Mutagenesis, Site-Directed · Plasmids · Precipitin Tests · Transformation, Genetic · Variation (Genetics) · Aspergillus awamori · Fungi · Fusarium · Fusarium solani · Saccharomyces cerevisiae
[Abstract]

8 Negative Regulation of Human Fibrinogen Gene Expression by Peroxisome Proliferator-activated Receptor α Agonists via Inhibition of CCAAT Box/Enhancer-binding Protein β
article 2001    
Author: Gervois, P. · Vu-Dac, N. · Kleemann, R. · Kockx, M. · Dubois, G. · Laine, B. · Kosykh, V. · Fruchart, J.C. · Kooistra, T. · Staels, B.
Keywords: Western blotting · Blotting, Northern · Blotting, Western · CCAAT-Enhancer-Binding Protein-alpha · Cell Line · Dose-Response Relationship, Drug · Down-Regulation · Fibrinogen · Hepatocytes · Humans · Inflammation · Interleukin-6 · Liver · Mutagenesis, Site-Directed · Nuclear Receptor Coactivator 2 · Peroxisome Proliferators · Plasmids · Precipitin Tests · Promoter Regions (Genetics) · Pyrimidines · Receptors, Cytoplasmic and Nuclear · Transcription Factors · Transcription, Genetic · Transfection · Tumor Cells, Cultured
[PDF] [Abstract]

9 Biochemical and molecular characterization of Lactobacillus reuteri 121 reuteransucrase
article 2004    
Author: Kralj, S. · Geel-Schutten, G.H. van · Maarel, M.J.E.C. van der · Dijkhuizen, L.
Keywords: Nutrition · Food technology · Amino acid · Asparagine · Aspartic acid · Glucan · Glucoside · Glutamic acid · Glutamine · Glycosyltransferase · Oligosaccharide · Reuteransucrase · Sucrase · Sucrose · Unclassified drug · Amino terminal sequence · Bioassay · Carbohydrate synthesis · Carboxy terminal sequence · Catalysis · Controlled study · Deletion mutant · Enzyme activity · Enzyme analysis · Enzyme inactivation · Enzyme kinetics · Lactobacillus reuteri · Leuconostoc · Molecular size · Nonhuman · Priority journal · Protein binding · Sequence alignment · Streptococcus · Amino Acid Sequence · Binding Sites · Gene Deletion · Glucans · Glycosyltransferases · Kinetics · Lactobacillus · Maltose · Molecular Sequence Data · Mutagenesis, Site-Directed · Sucrose · Lactobacillus · Lactobacillus reuteri · Leuconostoc · Streptococcus
[Abstract]

10 Site-directed mutagenesis study of the three catalytic residues of the fructosyltransferases of Lactobacillus reuteri 121
article 2004    
Author: Ozimek, L.K. · Hijum, S.A.F.T. van · Koningsveld, G.A. van · Maarel, M.J.E.C. van der · Geel-Schutten, G.H. van · Dijkhuizen, L.
Keywords: Food technology · Catalytic residue · Fructosyltransferase · Inulosucrase · Levansucrase · Mutagenesis · Acid · Base · Glycosidase · Levansucrase · Mutant protein · Stabilizing agent · Sucrase · Transferase · Bacterium mutant · Enzyme activity · Nonhuman · Site directed mutagenesis · Amino Acid Motifs · Amino Acid Sequence · Amino Acid Substitution · Catalysis · Circular Dichroism · Cloning, Molecular · Conserved Sequence · Escherichia coli · Gene Expression · Genes, Bacterial · Hexosyltransferases · Kinetics · Lactobacillus · Molecular Sequence Data · Mutagenesis, Site-Directed · Sequence Homology, Amino Acid · Substrate Specificity · Bacillus subtilis · Bacteria (microorganisms) · Lactobacillus reuteri
[PDF] [Abstract]

11 Exploring the active center of human acetylcholinesterase with stereomers of an organophosphorus inhibitor with two chiral centers
article 1999    
Author: Ordentlich, A. · Barak, D. · Kronman, C. · Benschop, H.P. · Jong, L.P.A. de · Ariel, N. · Barak, R. · Segall, Y. · Velan, B. · Shafferman, A.
Keywords: Cholinesterase reactivator · Soman · Binding site · Chirality · Dealkylation · Enzyme activity · Enzyme analysis · Enzyme binding · Enzyme specificity · Enzyme structure · Enzyme substrate · Human cell · Protein expression · Stereochemistry · Stereoisomerism · Alkylation · Amino acid substitution · Binding sites · Cell line · Cholinesterase inhibitors · Humans · Hydrogen Bonding · Kidney · Macromolecular substances · Models, Molecular · Mutagenesis, Site-Directed · Stereoisomerism · Acetylcholinesterase, EC 3.1.1.7 · Alanine, 56-41-7 · Phenylalanine, 63-91-2 · Phosphorus, 7723-14-0 · Soman, 96-64-0
[Abstract]

12 Retinoids increase human apo C-III expression at the transcriptional level via the retinoid X receptor : Contribution to the hypertriglyceridemic action of retinoids
article 1998    
Author: Vu-Dac, N. · Gervois, P. · Torra, I.P. · Fruchart, J.C. · Kosykh, V. · Kooistra, T. · Princen, H.M.G. · Dallongeville, J. · Staels, B.
Keywords: Health · Gene regulation · Hyperlipidemia · Nuclear receptors · Retinoids · Triglycerides · Adult · Apolipoprotein C-III · Apolipoproteins C · Benzoates · Carcinoma, Hepatocellular · Cells, Cultured · Dimerization · Double-Blind Method · Gene Expression Regulation · Hela Cells · Humans · Hypertriglyceridemia · Isotretinoin · Liver · Liver Neoplasms · Male · Mutagenesis, Site-Directed · Promoter Regions (Genetics) · Receptors, Cytoplasmic and Nuclear · Receptors, Retinoic Acid · Recombinant Fusion Proteins · Regulatory Sequences, Nucleic Acid · Retinoid X Receptors · Retinoids · Tetrahydronaphthalenes · Transcription Factors · Transcription, Genetic · Transfection · Tumor Cells, Cultured
[PDF] [Abstract]

13 Mutational analysis of pea lectin: Substitution of Asn125 for Asp in the monosaccharide-binding site eliminates mannose/glucose-binding activity
article 1992    
Author: Eijsden, R.R. van · Hoedemaeker, F.J. · Díaz, C.L. · Lugtenberg, B.J.J. · Sylvia Pater, B. de · Kijne, J.W.
Keywords: mutagenesis · pea lectin · sugar binding · mannose · oligodeoxyribonucleotide · pea lectin · phytohemagglutinin · amino acid sequence · binding site · chemistry · comparative study · Escherichia coli · genetics · legume · medicinal plant · metabolism · molecular genetics · nucleotide sequence · site directed mutagenesis · structure activity relation · Amino Acid Sequence · Asparagine · Aspartic Acid · Base Sequence · Binding Sites · Calcium · Comparative Study · DNA Mutational Analysis · Escherichia coli · Fabaceae · Glucose · Lectins · Manganese · Mannose · Molecular Sequence Data · Mutagenesis, Site-Directed · Oligodeoxyribonucleotides · Phytohemagglutinins · Plants, Medicinal · Sequence Alignment · Structure-Activity Relationship
[Abstract]

14 Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site
article 2003    
Author: Arand, M. · Hallberg, B.M. · Zou, J. · Bergfors, T. · Oesch, F. · Werf, M.J. van der · Bont, J.A.M. de · Jones, T.A. · Mowbray, S.L.
Keywords: Biology · Biotechnology · Crystal structure · Enantioselectivity · Epoxide hydrolase · Mechanism · Monoterpene degradation · bacterial enzyme · limonene 1,2 epoxide hydrolase · selenomethionine · unclassified drug · valpromide · alpha helix · article · beta sheet · catalysis · chemical reaction · crystal structure · detoxification · drug protein binding · enantioselectivity · enzyme active site · enzyme metabolism · enzyme structure · enzyme substrate complex · nonhuman · priority journal · reaction analysis · Rhodococcus erythropolis · site directed mutagenesis · Amino Acid Sequence · Bacterial Proteins · Catalytic Domain · Crystallography, X-Ray · Dimerization · Epoxide Hydrolases · Models, Molecular · Molecular Sequence Data · Mutagenesis, Site-Directed · Protein Subunits · Recombinant Proteins · Rhodococcus · Sequence Homology, Amino Acid · Actinobacteria (class) · Bacteria (microorganisms) · Rhodococcus · Rhodococcus erythropolis · uncultured actinomycete
[Abstract]

15 Generation of a recombinant apolipoprotein E variant with improved biological functions: Hydrophobic residues (LEU-261, TRP-264, PHE-265, LEU-268, VAL-269) of apoE can account for the apoE-induced hypertriglyceridemia
article 2005    
Author: Kypreos, K.E. · Dijk, K.W. van · Havekes, L.M. · Zannis, V.I.
Keywords: Biology · Biomedical Research · Amino acids · Biochemistry · Cholesterol · Genes · Molecular biology · Mutagenesis · Alanines · Apolipoprotein E (apoE) · High density lipoprotein (HDL) · Ultracentrifugation analysis · Proteins · High density lipoprotein · Leucine · Lipoprotein lipase · Phenylalanine · Recombinant apolipoprotein E · Recombinant protein · Triacylglycerol · Tryptophan · Unclassified drug · Valine · Very low density lipoprotein · Adenovirus · Amino acid substitution · Animal experiment · Carboxy terminal sequence · Controlled study · Density gradient centrifugation · Electron microscopy · Gene transfer · Human cell · Hydrophobicity · Hyperlipidemia · Hypertriglyceridemia · Mouse · Mutation · Nonhuman · Protein expression · Protein function · Animals · Apolipoproteins E · Cholesterol · Female · Humans · Hypertriglyceridemia · Lipoproteins, HDL · Lipoproteins, VLDL · Liver · Mice · Mice, Knockout · Mutagenesis, Site-Directed · Mutation, Missense · Peptide Fragments · Recombinant Proteins · Transgenes · Triglycerides
[PDF] [Abstract]

16 Testosterone 15β-hydroxylation by solvent tolerant Pseudomonas putida S12
article 2007    
Author: Ruijssenaars, H.J. · Sperling, E.M.G.M. · Wiegerinck, P.H.G. · Brands, F.T.L. · Wery, J. · Bont, J.A.M.de
Keywords: Biology · Biotechnology · CYP106A2 · Fer · Pseudomonas putida · Solvent tolerance · Steroid synthesis · Whole cell biocatalyst · Biocatalysts · Enzyme kinetics · Hydroxylation · Optimization · Solvents · Pseudomonas putida · Solvent tolerance · Steroid synthesis · Whole cell biocatalysts · Drug products · 15 beta hydroxylase · bacterial protein · cytochrome P450 · organic solvent · steroid monooxygenase · testosterone · threonine · valine · 15beta hydroxylase CYP106A2, Bacillus megaterium · 15beta-hydroxylase CYP106A2, Bacillus megaterium · solvent · article · Bacillus subtilis · biocatalyst · biotransformation · chemical structure · controlled study · high performance liquid chromatography · nonhuman · nuclear magnetic resonance · priority journal · protein expression · Pseudomonas putida · steroid hydroxylation · steroidogenesis · biological model · enzyme specificity · evaluation · gene expression regulation · genetics · hydroxylation · metabolism · site directed mutagenesis · transgenic organism · Bacillus megaterium · Bacillus subtilis · Pseudomonas putida · Bacterial Proteins · Cytochrome P-450 Enzyme System · Gene Expression Regulation, Bacterial · Hydroxylation · Models, Biological · Mutagenesis, Site-Directed · Organisms, Genetically Modified · Pseudomonas putida · Solvents · Substrate Specificity · Testosterone
[Abstract]

Search results also available in MS Excel format.

Showing 1 to 16 of 16 found. | Sort by date