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The wire grid biosensor for nucleic acid testing

Author: Schmidt, M.R.
Type:report
Date:2009-09-30
Publisher: Philips Research
Institution: Philips Research
Identifier: tn2009-00302
Keywords: dna · fluorescence imaging · microarrays · molecular diagnostics · optical biosensing
Rights: (c) Koninklijke Philips Electronics N.V.

Abstract

The rapidly growing field of targeted diagnostics is serving an increasing spectrum of mature clinical applications, supported by the continuous development of new facilitating equipment. The wire grid microarray is a novel concept for targeted nucleic acid and protein analysis, developed at Philips Research. This thesis demonstrates theversatility of the wire grid microarray regarding its compatibilitywith a variety of optical imaging systems, in particular: A compactwide-field fluorescence imaging setup, composed of off-the-shelf components, a sophisticated wide-field imaging setup with a highly sensitive CCD camera, and a confocal laser scanning fluorescence imagingsetup. Using these systems, it is shown that the wire grid microarray allows the detection of genomic nucleic acids, and a real-time monitoring of the hybridization of synthetic nucleic acids, on a surface. This provides valuable kinetic information about the hybridization process. The main enabling feature of the wire grid microarray isthe strong suppression of the background fluorescence. The suppression of the background is shown to be at least 5 times higher than for conventional confocal scanning.

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