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Isolation and characterization of promoter regions from Streptococcus gordonii CH1

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Author: Vriesema, A.J.M. · Dankert, J. · Zaat, S.A.J.
Type:article
Date:1999
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Current Microbiology, 6, 39, 321-326
Identifier: 235218
doi: doi:10.1007/s002849900466
Keywords: Nutrition · Bacterial DNA · Iron · Membrane protein · Bacterial gene · Bacterial virulence · Bacterium isolation · DNA sequence · Genetic transcription · Molecular cloning · Nonhuman · Nucleotide sequence · Open reading frame · Opportunistic infection · Priority journal · Promoter region · Streptococcus gordonii · Amino Acid Sequence · Base Sequence · DNA, Bacterial · Gene Expression Regulation, Bacterial · Iron · Molecular Sequence Data · Promoter Regions (Genetics) · Sequence Analysis, DNA · Streptococcus · Transcription, Genetic · Bacteria (microorganisms) · Escherichia coli · Posibacteria · Prokaryota · Streptococcus gordonii

Abstract

We aimed to identify transcription signal sequences from Streptococcus gordonii strain CH1 by random chromosomal cloning. Five genomic fragments from a Sau3A digest, which constitutively activated transcription of a promoterless spectinomycin resistance gene in this strain, were isolated and characterized. Additionally, one promoter fragment was isolated that was specifically activated under iron-limiting conditions. A sequence motif with similarity to the consensus for Fur-binding regulatory DNA sequences (Fur box) in Escherichia coli was detected within the putative promoter region. The open reading frame downstream of this region possibly encodes a transmembrane protein involved in iron uptake.