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Endothelium specific matrilysin (MMP-7) expression in human cancers

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Author: Sier, C.F.M. · Hawinkels, L.J.A.C. · Zijlmans, H.J.M.A.A. · Zuidwijk, K. · Jonge de · Muller, E.S.M. · Ferreira, V. · Hanemaaijer, R. · Mulder-Stapel, A.A. · Kenter, G.G. · Verspaget, H.W. · Gorter, A.
Type:article
Date:2008
Institution: TNO Kwaliteit van Leven
Source:Matrix Biology, 3, 27, 267-271
Identifier: 240728
doi: doi:10.1016/j.matbio.2007.10.006
Keywords: Health · Physiological Sciences · Angiogenesis · Breast · Carcinoma · Cervix · Colon · Matrix metalloproteinase · Prostate · Stomach · CD34 antigen · endoglin · matrilysin · vasculotropin · adult · aged · angiogenesis · antigen expression · article · cancer cell · cancer growth · cancer patient · cancer survival · endothelium cell · female · human · human cell · human tissue · immunohistochemistry · in vitro study · major clinical study · malignant neoplastic disease · priority journal · protein expression · reverse transcription polymerase chain reaction · tumor vascularization · uterine cervix cancer · Carcinoma · Cell Line, Tumor · Endothelium · Female · Gene Expression Regulation, Enzymologic · Gene Expression Regulation, Neoplastic · Humans · Matrix Metalloproteinase 7 · Neoplasm Invasiveness · Neovascularization, Pathologic · Spheroids, Cellular · Time Factors · Tissue Distribution · Umbilical Veins · Uterine Cervical Neoplasms

Abstract

Over-expression of matrilysin (MMP-7) is predominantly associated with epithelial (pre)malignant cells. In the present study MMP-7 expression is also found in endothelial cells in various human cancer types. Endothelial MMP-7 was associated with CD34 and/or CD105 expression. These immunohistochemical data were confirmed by RT-PCR on VEGF-stimulated endothelial cells. In addition, MMP-7 was also identified in sprouting endothelial cells in vitro. The potential clinical relevance of endothelial MMP-7 was assessed for cervical cancer patients by evaluating the association with overall survival. In contrast to MMP-7 in malignant epithelial cells, MMP-7 expression in endothelial cells showed a significant association with poor survival (LR 5.12, P = 0.02, n = 30). Our data suggest that MMP-7 is involved in tumor angiogenesis, thereby contributing to malignant growth and hence associated with decreased survival. © 2007 International Society of Matrix Biology.