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Accumulation of advanced glycation end products decreases collagen turnover by bovine chondrocytes

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Author: Groot, J. de · Verzijl, N. · Budde, M. · Bijlsma, J.W.J. · Lafeber, F.P.J.G. · TeKoppele, J.M.
Institution: Gaubius Instituut
Source:Experimental Cell Research, 2, 266, 303-310
Identifier: 236132
doi: doi:10.1006/excr.2001.5224
Keywords: Biology · Biomedical Research · Advanced glycation end product · Alginate culture · Cartilage · Chondrocyte · Collagen · Matrix metalloproteinase · Turnover · advanced glycation end product · alginic acid · collagen · matrix metalloproteinase · ribose · animal cell · article · articular cartilage · cartilage cell · cattle · cell culture · collagen defect · collagen degradation · collagen synthesis · controlled study · culture medium · enzyme activity · extracellular matrix · nonhuman · osteoarthritis · priority journal · reaction time · Animals · Cattle · Cells, Cultured · Chondrocytes · Collagen · Enzyme Precursors · Extracellular Matrix · Glycosylation End Products, Advanced · Matrix Metalloproteinases · Osteoarthritis · Ribose · Bovinae


The integrity of the collagen network is essential for articular cartilage to fulfill its function in load support and distribution. Damage to the collagen network is one of the first characteristics of osteoarthritis. Since extensive collagen damage is considered irreversible, it is crucial that chondrocytes maintain a functional collagen network. We investigated the effects of advanced glycation end products (AGEs) on the turnover of collagen by articular cartilage chondrocytes. Increased AGE levels (by culturing in the presence of ribose) resulted in decreased collagen synthesis (P < 0.05) and decreased MMP-mediated collagen degradation (P < 0.02). The latter could be attributed to increased resistance of the collagen network to MMPs (P < 0.05) as well as the decreased production of MMPs by chondrocytes (P < 0.02). Turnover of a protein is determined by its synthesis and degradation rates and therefore these data indicate that collagen turnover is decreased at enhanced AGE levels. Since AGE levels in human cartilage increase ∼50 fold between age 20 and 80, cartilage collagen turnover likely decreases with increasing age. Impaired collagen turnover adversely affects the capacity of chondrocytes to remodel and/or repair its extracellular matrix. Consequently, age-related accumulation of AGE (via decreased collagen turnover) may contribute to the development of cartilage damage in osteoarthritis. © 2001 Academic Press.